The effect of the antibody used and method of quantification on oral mucosal vascularity

Elgazzar, R.F.; Macluskey, Michaelina; Ogden, Graham S.

doi:10.1016/j.ijom.2005.03.004

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…We did not find any difference (data not shown) in density, area, and perimeter when analyzed site wise (buccal mucosa, tongue and others). This is similar to a previous study, in which anatomical site showed no difference in MVD using vWF [19]. Hence, it is less likely that our results are affected by inclusion of carcinomas of different oral sites.…”

Section: Angiogenesis and Metastasissupporting

confidence: 92%

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Role of angiogenesis in oral squamous cell carcinoma development and metastasis: an immunohistochemical study

et al. 2011

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Although a few studies have shown that vascularity is increased from normal mucosa to dysplasia to carcinoma suggesting that disease progression in the oral mucosa is accompanied by angiogenesis. The role in lymph node metastasis in oral squamous cell carcinoma (OSCC) is equivocal. Role of angiogenesis in OSCC development and metastasis is evaluated in this study. This retrospective study of 50 samples consisted of 9 normal buccal mucosa, 22 leukoplakias, and 19 OSCC. Polyclonal antibodies to von-Willebrand factor were used to highlight the microvessels. Images were captured and morphometric image analysis was done for microvessel density (MVD), area, and perimeter. Highest, as well as mean values of these three parameters were compared. MVD and perimeter, but not area, are significantly different between normal mucosa and OSCC, and leukoplakia and OSCC. There were no differences between normal mucosa and leukoplakia. MVD, area, and perimeter were not significantly different between the OSCC with and without lymph node metastasis. The highest and mean values of MVD are significantly correlated. In the development of OSCC, angiogenic phenotypic change occurs in carcinomas rather than in the pre-cancerous stage, and quantification of angiogenesis in OSCC does not predict the risk of lymph node metastasis.

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Section: Angiogenesis and Metastasissupporting

confidence: 92%

“…Others [19] recommend use of vWF. Within paraffin embedded tissues vWF is more potent than CD31 [19][20] or a close correlation exists between vWF and CD34/ CD31 staining [21][22]. Irrespective of methodology, MVD in seven out of 10 studies with vWF showed no relation to LN status (Table 5).…”

Section: Angiogenesis and Metastasismentioning

confidence: 99%

Role of angiogenesis in oral squamous cell carcinoma development and metastasis: an immunohistochemical study

et al. 2011

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“…Different methods are available for the evaluation of angiogenesis in biologic material, including MVC and the determination of microvessel density and volume. [36][37][38] Among these methods, MVC is an easy technique that has prognostic relevance in different tumors. 38,39 One widely used angiogenic marker is endoglin, or CD105, which was originally identified as a human endothelial marker although subsequent studies demonstrated that this cell surface antigen is also expressed by macrophages, erythrocyte precursors, and stromal cells.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of nuclear factor κB, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts

Santos¹,

Aquino²,

Barreto³

et al. 2011

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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“…20 In this manner, it is possible to indirectly establish the angiogenic activity of a tissue. 20,21 To our knowledge, there are no studies analyzing the expression of VEGF in PGs, RCs, and RRCs. Therefore, the aim of the present study was to assess and compare the immunoexpression of VEGF in these lesions, relating it to the angiogenic index and the intensity of the inflammatory infiltrate.…”

mentioning

confidence: 99%

Immunoexpression of vascular endothelial growth factor in periapical granulomas, radicular cysts, and residual radicular cysts

Nonaka¹,

Maia²,

Nascimento³

et al. 2008

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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The effect of the antibody used and method of quantification on oral mucosal vascularity

Cited by 4 publications

References 16 publications

Role of angiogenesis in oral squamous cell carcinoma development and metastasis: an immunohistochemical study

Role of angiogenesis in oral squamous cell carcinoma development and metastasis: an immunohistochemical study

Immunohistochemical expression of nuclear factor κB, matrix metalloproteinase 9, and endoglin (CD105) in odontogenic keratocysts, dentigerous cysts, and radicular cysts

Immunoexpression of vascular endothelial growth factor in periapical granulomas, radicular cysts, and residual radicular cysts

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