Perilla oil rich in α-linolenic acid inhibits neuronal apoptosis and the expression of inflammation-mediator protein in apoE KO mice

Seong, Jong-Hwan; Song, Yeong Ok

doi:10.1016/j.bcab.2012.01.001

Cited by 16 publications

(10 citation statements)

References 27 publications

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“…Perilla oil contains fatty acids including linolenic acid (C18:3), linoleic acid (C18:2), palmitic acid (C16:1), oleic acid (C18:1) and stearic acid (C18:0) [11,12], and perilla seeds contain the phenolics rosmarinic acid, rosmarinic acid-3-O-glucoside, caffeic acid, ferulic acid, and caffeic acid-3-O-glucoside and the flavonoids apigenin, luteolin, and catechin [13]. In addition, perilla oil has been reported to have meaningful pharmacological effects in cancer, diabetes, asthma, microbial infectious diseases, inflammation, oxidative stress-related diseases, and cardiovascular disease [12,14], and perilla oil supplementation in diet was found to improve loperamide-induced constipation symptoms in albino rats and to enhanced cognitive function in elderly patients with mild cognitive impairment [15,16].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

et al. 2020

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Positive physiological benefits of several plant oils on the UV-induced photoaging have been reported in some cell lines and model mice, but perilla oil collected from the seeds of Perilla frutescens L. has not been investigated in this context. To study the therapeutic effects of cold-pressed perilla oil (CPO) on UV-induced photoaging in vitro and in vivo, UV-induced cellular damage and cutaneous photoaging were assessed in normal human dermal fibroblasts (NHDFs) and HR-1 hairless mice. CPO contained five major fatty acids including linolenic acid (64.11%), oleic acid (16.34%), linoleic acid (11.87%), palmitic acid (5.06%), and stearic acid (2.48%). UV-induced reductions in NHDF cell viability, ROS production, SOD activity, and G2/M cell cycle arrest were remarkably improved in UV + CPO treated NHDF cells as compared with UV + Vehicle treated controls. Also, UV-induced increases in MMP-1 protein and galactosidase levels were remarkably suppressed by CPO. In UV-radiated hairless mice, topical application of CPO inhibited an increase in wrinkle formation, transepidermal water loss (TEWL), erythema value, hydration and melanin index on dorsal skin of UVB-irradiated hairless mice. CPO was observed to similarly suppress UV-induced increases in epidermal thickness, mast cell numbers, and galactosidase and MMP-3 mRNA levels. These results suggest CPO has therapeutic potential in terms of protecting against skin photoaging by regulating skin morphology, histopathology and oxidative status.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

et al. 2020

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“…Therefore, we speculated that the cognitive improvement against Aβ could be mediated by the anti-oxidative activity of PO and ALA. Previous studies demonstrated that PO and ALA ameliorate the oxidative stress by the inhibiting lipid peroxidation or regulating nuclear factor kappa B [ 13 14 15 ]. In the rat brain, H 2 O 2 , which is generated from the superoxide anion (O 2 − ), induces mitochondrial dysfunction resulting in cell death [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of perilla oil and alpha linolenic acid on SH-SY5Y neuronal cell death induced by hydrogen peroxide

et al. 2018

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BACKGROUND/OBJECTIVEOxidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. The aim of present study was to investigate the neuroprotective effects of perilla oil (PO) and its active component, alpha-linolenic acid (ALA), against hydrogen peroxide (H2O2)-induced oxidative stress in SH-SY5Y neuronal cells.MATERIALS/METHODSThe SH-SY5Y human neuroblastoma cells exposed to 250 µM H2O2 for 24 h were treated with different concentrations of PO (25, 125, 250 and 500 µg/mL) and its major fatty acid, ALA (1, 2.5, 5 and 25 µ/mL). We examined the effects of PO and ALA on H2O2-induced cell viability, lactate dehydrogenase (LDH) release, and nuclear condensation. Moreover, we determined whether PO and ALA regulated the apoptosis-related protein expressions, such as cleaved-poly ADP ribose polymerase (PARP), cleaved caspase-9 and -3, BCL-2 and BAX.RESULTSTreatment of H2O2 resulted in decreased cell viability, increased LDH release, and increase in the nuclei condensation as indicated by Hoechst 33342 staining. However, PO and ALA treatment significantly attenuated the neuronal cell death, indicating that PO and ALA potently blocked the H2O2-induced neuronal apoptosis. Furthermore, cleaved-PARP, cleaved caspase-9 and -3 activations were significantly decreased in the presence of PO and ALA, and the H2O2-mediated up-regulated BAX/BCL-2 ratio was blocked after treatment with PO and ALA.CONCLUSIONSPO and its main fatty acid, ALA, exerted the protective activity from neuronal oxidative stress induced by H2O2. They regulated apoptotic pathway in neuronal cell death by alleviation of BAX/BCL-2 ratio, and down-regulation of cleaved-PARP and cleaved caspase-9 and -3. Although further studies are required to verify the protective mechanisms of PO and ALA from neuronal damage, PO and ALA are the promising agent against oxidative stress-induced apoptotic neuronal cell death.

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“…Perilla seeds contain high levels of ␣-linolenic acid (ALA, C18:3) (Shin and Kim, 1994;Seong and Song, 2012), ranging from 52.58 to 61.98% (Zhou et al, 2014;Ciftci et al, 2012). Studies have reported that increased dietary intake levels of ALA, a precursor of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have beneficial effects in human health (Lee and Song, 2012).…”

Section: Introductionmentioning

confidence: 99%

Optimization of ultrasound-assisted hexane extraction of perilla oil using response surface methodology

Zhang

Hou

et al. 2015

Industrial Crops and Products

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Perilla oil rich in α-linolenic acid inhibits neuronal apoptosis and the expression of inflammation-mediator protein in apoE KO mice

Cited by 16 publications

References 27 publications

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

Protective effects of perilla oil and alpha linolenic acid on SH-SY5Y neuronal cell death induced by hydrogen peroxide

Optimization of ultrasound-assisted hexane extraction of perilla oil using response surface methodology

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