1992
DOI: 10.1007/bf00197045
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Periodic deposition of arabinogalactan epitopes in the cell wall of pollen tubes of Nicotiana tabacum L.

Abstract: The monoclonal antibodies MAC 207 and JIM 8, recognizing arabinogalactan epitopes, were used to localize the corresponding antigenic sites in pollen tubes of Nicotiana tabacum L. grown in vitro or semi in vivo. The analysis of the immunofluorescence labelling was performed by means of confocal laser scanning microscopy. Most pollen tubes were labelled along their length, with the exception of the tip region, in a ring-like pattern with remarkable periodicity. The diameter of the rings was approx. 12 μm and the… Show more

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Cited by 82 publications
(69 citation statements)
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“…In the presence of 1 HIM EGTA, the apparent binding of AGP to pectin is reduced, which suggests that the interaction between AGP and pectin may be Ca 2+ dependent and raises the intriguing possibility of calcium-dependent cell-matrix attachment. Two examples that also support the idea of their interaction are the dramatic co-localization of pectin and AGP in Arabidopsis pollen grains and tobacco pollen tube walls (Li et al, 1992;Van Aelst and Van Went, 1992). Van Aeslt and Van Went (1992) demonstrated that MAC 207 and JIM 5 (an anti-pectin monoclonal antibody) both specifically label the intine region of Arabidopsis thaliana pollen grains adjacent to the plasma membrane.…”
Section: Agps May Be Pectin-binding Proteinsmentioning
confidence: 87%
See 1 more Smart Citation
“…In the presence of 1 HIM EGTA, the apparent binding of AGP to pectin is reduced, which suggests that the interaction between AGP and pectin may be Ca 2+ dependent and raises the intriguing possibility of calcium-dependent cell-matrix attachment. Two examples that also support the idea of their interaction are the dramatic co-localization of pectin and AGP in Arabidopsis pollen grains and tobacco pollen tube walls (Li et al, 1992;Van Aelst and Van Went, 1992). Van Aeslt and Van Went (1992) demonstrated that MAC 207 and JIM 5 (an anti-pectin monoclonal antibody) both specifically label the intine region of Arabidopsis thaliana pollen grains adjacent to the plasma membrane.…”
Section: Agps May Be Pectin-binding Proteinsmentioning
confidence: 87%
“…ing tobacco pollen tubes (Li et al, 1992); furthermore, they also recently demonstrated that these bands cross-react strongly with an anti-pectin monoclonal antibody (personal communication, Sixth Cell Wall Meeting, Nijmegen, The Netherlands, 1992). The functional significance of this possible AGP-pectin interaction awaits future study.…”
Section: Agps May Be Pectin-binding Proteinsmentioning
confidence: 96%
“…2B-C) as observed in other species such as lily (Mollet et al, 2002) or tobacco (Li et al, 1992). Moreover, pectic domains such as the the arabinan side chains of RG-I ( Fig.…”
Section: Arabidopsis Pollen Tubesmentioning
confidence: 54%
“…On the other hand, pulsating growth, typical of petunia and tobacco, is characterized by slower phases of growth followed by growth spurts (pulses) (Picton and Steer, 1982). It seems that the periods of slow growth coincide with the increased thickness by deposition of pectins and arabinogalactan proteins (AGPs) (Li et al, 1992(Li et al, , 1994, and the periods of pulse correspond to thinner cell wall formation (Li et al, , 1997Pierson et al, 1995), although lily pollen tubes show a uniform deposition of these wall components in the tube, even though they show a marked oscillation in growth rate. The mechanisms underlying the oscillating, or pulsating, growth are not completely understood yet, but it is clear that many important factors contribute to this process.…”
Section: Male Gametophytementioning
confidence: 99%