Periodontal disease may associate with breast cancer

Söder, Birgitta; Yakob, Maha; Meurman, Jukka H.; Andersson, Leif C.; Klinge, Björn; Söder, Per‐Östen

doi:10.1007/s10549-010-1221-4

Cited by 61 publications

(71 citation statements)

References 36 publications

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“…Recently, it has been proposed that periodontal disease may be associated with breast cancer (30). Oral microbiologic profile determination could be a suitable approach for management of the oral diseases.…”

Section: Discussionmentioning

confidence: 99%

Development and Comparison of Conventional PCR and SYBR Green Real Time PCR for Detection of Aggregatibacter actinomycetemcomitans and Tannerella for Sythensis

Soleimani

Zolfaghari

Morovvati

2013

Jundishapur J Microbiol

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Background: Aggregatibacter actinomycetemcomitans and Tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. The detection of these bacteria is needed for diagnosis and management of the mentioned diseases. Objectives: We aimed to develop and compare improved multiplex conventional and SYBR green real time PCR assays for a specific diagnosis of the organisms based on specific marker genes. Materials and Methods: Both PCR approaches were performed with primers targeting diagnostic genes of the organisms, hbpA gene of A. actinomycetemcomitans and 16S rRNA gene of T. forsythensis. For preparation of a stable positive control, the PCR products were cloned into pTZ57R/T plasmid. The test specificity was evaluated using the same PCR reactions but in the presence of genomes of various negative control bacteria. Results: As expected, agarose gel electrophoresis of PCR products of the hbpA and 16S rRNA genes showed 160 bp and 250bp bands respectively. Temperature melting analyses of the SYBR green real time PCR assays showed the Tm at 78.02 °C and 84.62 °C for hbpA and 16S rRNA genes respectively. The amplification results using negative control genomes as template was negative showing the specificity of both designed assays. The detection limits of the conventional PCR assay for hbpA and 16S rRNA genes were 5200 and 1200 copies of each gene, respectively. The designed SYBR green PCR assays tenfold increased the test sensitivity. Conclusions: The designed assays provide simple, reliable, and rapid procedures that identify two main periodontal pathogens. Theses assays are new diagnostic opportunities in our laboratory and also are working as important supplements of the current time consuming phenotypic assays.

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Section: Discussionmentioning

confidence: 99%

Development and Comparison of Conventional PCR and SYBR Green Real Time PCR for Detection of Aggregatibacter actinomycetemcomitans and Tannerella for Sythensis

Soleimani

Zolfaghari

Morovvati

2013

Jundishapur J Microbiol

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“…Thus, this interesting study of Söder et al [1] supports the need for further research to examine the role of vitamin D deficiency, as an avoidable risk factor, in those with periodontal disease and breast cancer.…”

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confidence: 52%

“…To the Editor, The recent article by Söder et al [1] found a statistically significant correlation between periodontal disease, related tooth loss and incidence of breast cancer. Periodontal disease with any missing molar teeth was associated with nearly a 12-fold increased risk of breast cancer than no missing molars.…”

mentioning

confidence: 98%

Low vitamin D status likely contributes to the link between periodontal disease and breast cancer

Grant

Boucher

2011

Breast Cancer Res Treat

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“…The authors suggested that the lack of increase in the risk of cancer after loss of more than 15 teeth may be due to the absence of a periodontal pathogen or due to presence of little or no remaining teeth. Studies have also shown that periodontal disease is also associated with other cancers such as pancreatic, colorectal, prostate, uterine and breast cancers (Michaud et al, 2007;Arora et al, 2010;Soder et al, 2011).…”

Section: Periodontitis and Cancermentioning

confidence: 99%