Periodontal tissues are targets for Sars-Cov-2: a post-mortem study

Matuck, Bruno Fernandes; Dolhnikoff, Marisa; Maia, Gilvan; Sendyk, Daniel Isaac; Zarpellon, Amanda; Gomes, Sara Costa; Duarte‐Neto, Amaro Nunes; Pinho, João Renato Rebello; Gomes‐Gouvêa, Michele Soares; Sousa, Suzana Orsini Machado de; Mauad, Thaís; Saldiva, Paulo; Braz‐Silva, Paulo Henrique; Silva, Luiz Fernando Ferraz da

doi:10.1080/20002297.2020.1848135

Cited by 83 publications

(102 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Concluded that the oral cavity is an important site for SARS-CoV-2 and saliva as a potential route of COVID-19 transmission from oral droplets containing infectious virus and infected cells. In another study by Matuck et al (2020) demonstrated the presence of SARS-CoV-2 in periodontal tissue in severely ill patients (26). The authors highlight that periodontal tissue can be a target for SARS-CoV-2, and contribute to the presence of the virus in saliva.…”

Section: Discussionmentioning

confidence: 93%

Toothbrushing with a dentifrice containing antimicrobial phthalocyanine derivative for the intraoral reduction of viral load of SARS-CoV-2: a pilot study

Poleti

Gregorio

Bistaffa

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: The aim of this study was to evaluate the effects of the toothbrushing with a dentifrice containing antimicrobial phthalocyanine derivative (APD) for the intraoral reduction of viral load of SARS-CoV-2.Material and methods: Twenty COVID-19 positive dentate patients were selected and toothbrushes with a dentifrice containing APD for 2 minutes. Self-collected samples of unstimulated saliva were carried out three times: T0 (baseline, before toothbrushing), T5 (5 minutes after toothbrushing), and T30 (30 minutes after toothbrushing). The analysis of RNA viral was performed by RT-PCR using TaqPath™ COVID-19 multiplex Real-Time RT-PCR test for detection of three viral genes (ORF1ab, N and S genes). Evaluation of the effects was based on difference in cycle threshold (Ct) value. Friedman's test and pairwise comparison with Bonferroni corrections were used, with a significance level of 5%. Results: The Ct values were significantly higher (p=0.020) at T30 in comparison to T0 and T5. The greatest difference in the Ct values was between T30 and T0 (3.83). Conclusion: This pilot study suggests that oral hygiene action associated with an antimicrobial chemical dentifrice may be an important tool for SARS-CoV2 viral load reduction in oral cavity.

show abstract

Section: Discussionmentioning

confidence: 93%

Toothbrushing with a dentifrice containing antimicrobial phthalocyanine derivative for the intraoral reduction of viral load of SARS-CoV-2: a pilot study

Poleti

Gregorio

Bistaffa

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The presence of SARS‐CoV‐2 in saliva may be related to viral proliferation and RNA secretion in any cells and tissues involved in production of salivary components, such as periodontal tissue, salivary glands, and cells of the upper respiratory tract [5]. For instance, we have previously demonstrated the presence of SARS‐CoV‐2 RNA in periodontal tissue [6]. Determining how each tissue contributes as a reservoir for SARS‐CoV‐2 may be a path towards better understanding of the SARS‐CoV‐2 profile in saliva and for developing strategies for improving diagnosis, as well as for mitigating contamination through salivary droplets.…”

Section: Introductionmentioning

confidence: 99%

Salivary glands are a target for SARS‐CoV‐2: a source for saliva contamination

Matuck

Dolhnikoff

Duarte‐Neto

et al. 2021

The Journal of Pathology

Self Cite

View full text Add to dashboard Cite

The ability of the new coronavirus SARS‐CoV‐2 to spread and contaminate is one of the determinants of the COVID‐19 pandemic status. SARS‐CoV‐2 has been detected in saliva consistently, with similar sensitivity to that observed in nasopharyngeal swabs. We conducted ultrasound‐guided postmortem biopsies in COVID‐19 fatal cases. Samples of salivary glands (SGs; parotid, submandibular, and minor) were obtained. We analyzed samples using RT‐qPCR, immunohistochemistry, electron microscopy, and histopathological analysis to identify SARS‐CoV‐2 and elucidate qualitative and quantitative viral profiles in salivary glands. The study included 13 female and 11 male patients, with a mean age of 53.12 years (range 8–83 years). RT‐qPCR for SARS‐CoV‐2 was positive in 30 SG samples from 18 patients (60% of total SG samples and 75% of all cases). Ultrastructural analyses showed spherical 70–100 nm viral particles, consistent in size and shape with the Coronaviridae family, in the ductal lining cell cytoplasm, acinar cells, and ductal lumen of SGs. There was also degeneration of organelles in infected cells and the presence of a cluster of nucleocapsids, which suggests viral replication in SG cells. Qualitative histopathological analysis showed morphologic alterations in the duct lining epithelium characterized by cytoplasmic and nuclear vacuolization, as well as nuclear pleomorphism. Acinar cells showed degenerative changes of the zymogen granules and enlarged nuclei. Ductal epithelium and serous acinar cells showed intense expression of ACE2 and TMPRSS receptors. An anti‐SARS‐CoV‐2 antibody was positive in 8 (53%) of the 15 tested cases in duct lining epithelial cells and acinar cells of major SGs. Only two minor salivary glands were positive for SARS‐CoV‐2 by immunohistochemistry. Salivary glands are a reservoir for SARS‐CoV‐2 and provide a pathophysiological background for studies that indicate the use of saliva as a diagnostic method for COVID‐19 and highlight this biological fluid's role in spreading the disease. © 2021 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

show abstract

“…Only one study focused on identifying the presence of SARS-CoV-2 in oral tissues by means of PCR. The study design most used was “in silico”, followed by “in vivo” and finally “in vitro” [ 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. Most of the articles showed a combination of two or more of the aforementioned study designs.…”

Section: Resultsmentioning

confidence: 99%

Presence of SARS-CoV-2 and Its Entry Factors in Oral Tissues and Cells: A Systematic Review

et al. 2021

View full text Add to dashboard Cite

Background and Objectives: The aim of this systematic review is to summarize the current data about the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its entry factors in oral tissues and cells. Materials and Methods: This systematic review was carried out based on the Preferred Reporting Items for a Systematic Review and Meta-Analysis (PRISMA). Three databases were analyzed (Pubmed, Web of science and Scopus) by three independent researchers. From the 18 identified studies, 10 of them met the inclusion criteria. The presence of SARS-CoV-2 or its entry factors (angiotensin-converting enzyme II (ACE2), transmembrane serine proteases (TMPRSS), and furin) was analyzed in these 10 studies during the pandemic. Results: ACE2 expression was analyzed in 9 of the 10 studies. ACE2 is expressed mainly in the tongue, oral mucosa, salivary glands and epithelial cells. The expression of the TMPRSS2 gene or protein was analyzed in 6 studies. These studies reported that the expression of TMPRSS2 was mainly in the salivary glands, tongue, sulcular epithelium and oral mucosa; as well as in cells of the salivary glands (ductal, acinar and myoepithelial cells) and the tongue (the spinous-based cell layer, horny layer and the epithelial surface). Other TMPRSS were also reported. The expression of TMPRSS3, TMPRSS4, TMPRSS5, TMPRSS7 and TMPRSS11D was reported mainly in salivary glands and in epithelial-type cells. Furan expression was analyzed in three studies. The expression of furin was detected mainly in epithelial cells of the tongue. A variety of methods were used to carry out the detection of SARS-CoV-2 or its input molecules. Conclusions: These results show that SARS-CoV-2 can infect a wide variety of oral tissues and cells, and that together with the theories dedicated to explaining the oral symptoms present in SARS-CoV-2 positive patients, it provides us with a good scientific basis for understanding the virus infection in the oral cavity and its consequences.

show abstract

Periodontal tissues are targets for Sars-Cov-2: a post-mortem study

Cited by 83 publications

References 30 publications

Toothbrushing with a dentifrice containing antimicrobial phthalocyanine derivative for the intraoral reduction of viral load of SARS-CoV-2: a pilot study

Toothbrushing with a dentifrice containing antimicrobial phthalocyanine derivative for the intraoral reduction of viral load of SARS-CoV-2: a pilot study

Salivary glands are a target for SARS‐CoV‐2: a source for saliva contamination

Presence of SARS-CoV-2 and Its Entry Factors in Oral Tissues and Cells: A Systematic Review

Contact Info

Product

Resources

About