Background:The association between periodontal status, oral hygiene status and severity of tooth wear lesion varies from community to community and also from occupation to occupation.Aim:To determine the association between periodontal status, oral hygiene status and tooth wear among the adult male population in Benin City, Nigeria.Subjects and Methods:This study was conducted among 65 male drivers, aged 25-64 years, with a mean age of 48.6 (9.1) years in an organized private motor park in Benin City between November 2011 and January 2012. The data collected through interview and clinical oral examination were age, educational status, driving experience, oral hygiene status, periodontal status and severity of tooth wear.Results:In this study, 13.8% (9/65) and 1.5% (1/65) of the participants had 4-5 mm and ≥6 mm periodontal pockets, respectively. A total of 15.4% (10/65) of the participants had poor oral hygiene status and 58.5% (38/65) of the participants had tooth wear lesion, with 15.8% (9/65) being severe tooth wear lesions (score 3). Participants with poorer oral hygiene and more severe tooth wear lesions significantly exhibited poorer periodontal status. There also existed a significant association between oral hygiene status and the severity of tooth wear lesion among the participants in this study.Conclusion:Data from this study revealed a statistically significant association between periodontal status, oral hygiene status and severity of tooth wear lesion among the participants.