Periodontopathic Bacterial Infection in Childhood

Kimura, Shigenobu; Ooshima, Takashi; Takiguchi, Miyako; Sasaki, Yumi; Amano, Atsuo; Morisaki, Ichijiro; Hamada, Shigeyuki

doi:10.1902/jop.2002.73.1.20

Cited by 73 publications

(106 citation statements)

References 42 publications

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“…The detection of A. actinomycetemcomitans is more frequent in children with discernible gingivitis than in children with no discernible gingivitis (9). We expected to detect the organism in 50% of children with no discernible gingivitis.…”

Section: Methodsmentioning

confidence: 92%

“…There are few data concerning the prevalence of P. gingivalis, A. actinomycetemcomitans, and T. forsythensis in the plaque of healthy children. The available prevalence data for these pathogens are also contradictory; for example, P. gingivalis has been isolated from the plaque of 80% of children during and after puberty (28), whereas other studies failed to detect P. gingivalis in prepubertal children (9,31). Although periodontal disease is rare in healthy children, it is important to investigate the presence of periodontal pathogens as the permanent teeth start to erupt.…”

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confidence: 99%

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Prevalence of Periodontal Pathogens in Dental Plaque of Children

et al. 2004

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Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Tannerella forsythensis have been implicated as the main etiological agents of periodontal disease. The purpose of this work was to estimate the prevalence of these organisms in plaque from children without gingivitis (group 1; n ‫؍‬ 65) and from those with gingivitis (group 2; n ‫؍‬ 53). Extracted DNA from plaque was subjected to two rounds of PCR targeting the 16S rRNA gene using both universal primers and species-specific primers. The results were as follows: group 1, P. gingivalis, 49%; A. actinomycetemcomitans, 55%; and T. forsythensis, 65%; group 2, P. gingivalis, 47%; A. actinomycetemcomitans, 59%; and T. forsythensis, 45%. T. forsythensis was detected more frequently in children with no gingivitis than in those with gingivitis (P ‫؍‬ 0.03). There was no significant difference between the two groups with respect to the presence of P. gingivalis or A. actinomycetemcomitans in either group (P > 0.05). Logistic regression analysis revealed that the odds of a patient having gingivitis were 2.3 times greater in the absence of T. forsythensis. In conclusion, the results of this study have shown that the three pathogens can be detected in the dental plaque of healthy children and of those with gingivitis and that T. forsythensis is associated with dental plaque at sites with no gingivitis.

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Section: Methodsmentioning

confidence: 92%

mentioning

confidence: 99%

Prevalence of Periodontal Pathogens in Dental Plaque of Children

et al. 2004

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“…The target bacteria were 4 periodontopathic species; P. gingivalis, P. intermedia, T. forsythia, and T. denticola, and 16S rRNA-based PCR assays were performed using species-specific PCR primers, as reported previously (Table 1). 22,24 The bacterium-specific primer (5′ -3′) sequences used in this study were as follows: P. gingivalis, sense, 5′ -TGTAGATGACTGATGGTGAAAACC-3′, and antisense, 5′-AGCCGCTGTCGAAAAGCCCA-3′ (575 bp).…”

Section: Pcr Analysismentioning

confidence: 99%

Prediction of periodontopathic bacteria in dental plaque of periodontal healthy subjects by measurement of volatile sulfur compounds in mouth air

Kishi

Ohara‐Nemoto

Takahashi

et al. 2013

Archives of Oral Biology

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Objectives:The aim of this study was to determine whether measurements of volatile sulfur compounds (VSCs) are useful to predict colonization of periodontopathic bacteria. For this purpose, we assessed the relationships among distributions of 4 species of periodontopathic bacteria in tongue coating and dental plaque, oral conditions including VSC concentration in mouth air, and smoking habit of periodontal healthy young subjects. Methods:The subjects were 108 young adults (mean age, 23.5±2.56 years) without clinical periodontal pockets. Information regarding smoking habit was obtained by interview. After VSC concentration in mouth air was measured with a portable sulfide monitor (Halimeter ® ), non-stimulated saliva flow and dental caries status were assessed, and tongue coating and dental plaque samples were collected from the subjects. The tongue coating samples were weighed to determine the amount. The colonization of Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola in both tongue coating and plaque samples was investigated using species-specific polymerase chain reaction assays.Results: Significant relationships were observed between the colonization of periodontopathic bacteria in tongue coating and plaque samples, especially that of P.gingivalis. VSC concentration showed the most significant association with colonization of P. gingivalis in both tongue coating and dental plaque. Logistic regression analysis demonstrated that the adjusted partial correlation coefficient [Exp(B)] values for VSC concentration with the colonization of P. gingivalis, P. intermedia, and T. denticola in dental plaque were 135, 35.4 and 10.4, respectively. In addition, smoking habit was also shown to 3 be a significant variable in regression models [Exp(B) = 6.19, 8.92, and 2.53, respectively].Therefore, receiver operating characteristic analysis was performed to predict the colonization of periodontal bacteria in dental plaque in the subjects divided by smoking habit. Based on our results, we found cut-off values that indicated likelihood ratios (LR) within the efficient range for positive findings in both groups. Conclusion:The present results demonstrated that measurement of VSC concentration in mouth air is a useful method to predict the presence of colonization of some periodontopathic bacteria in dental plaque.

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“…Nevertheless, the prevalence and severity of inflammation of the oral tissues (gingivitis and periodontitis) is low in healthy young children and gradually increases with increasing age (Matsson, 1993;Papaioannou et al, 2009). With increasing age, the proportions of periodontal pathogens also increase Kimura et al, 2002). Page and Shroeder (1976), reported the sequence of changes during the development of gingivitis and peridontitis under four stages, according to prominent histopathological signs.…”

Section: Histopathologymentioning

confidence: 99%