Periostin, a matricellular protein, accelerates cutaneous wound repair by activating dermal fibroblasts

Ontsuka, Kanako; Kotobuki, Yorihisa; Shiraishi, Hiroshi; Serada, Satoshi; Ohta, Shoichiro; Tanemura, Atsushi; Yang, Lingli; Fujimoto, Minoru; Arima, Kazuhiko; Suzuki, Shingo; Murota, Hiroyuki; Toda, Shuji; Kudo, Akira; Conway, Simon J.; Narisawa, Yutaka; Katayama, Ichiro; Izuhara, Kenji; Naka, Tetsuji

doi:10.1111/j.1600-0625.2012.01454.x

Cited by 105 publications

(119 citation statements)

References 55 publications

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“…The direct or indirect production or promotion of TGF-beta could be incriminated for Col 1a2 transcription by competent cells (17). However, other factors that may induce profibrotic mediators by intermediate cell types could also be considered (5,(18)(19)(20). Naturally, because of the central role of macrophages in wound healing biology, these hypotheses are not mutually exclusive.…”

Section: Discussionmentioning

confidence: 99%

Wound‐associated macrophages control collagen 1α2 transcription during the early stages of skin wound healing

Rodero

Legrand

Bou‐Gharios

et al. 2013

Experimental Dermatology

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Additional Supporting Information may be found in the online version of this article:Data S1. Materials and methods. Figure S1. Effect of galanin and GALP applied to the basolateral side of NCL-SG3 cells on short-circuit current. Abstract: Wound-associated fibrosis is important to provide tensile strength upon wound healing but at the same time is detrimental to proper tissue regeneration. To date, there is no clear evidence of the role of macrophages and their subpopulations in the control of the kinetics of collagen production during wound healing. To evaluate in vivo the contribution of macrophages in collagen transcription, we depleted macrophages after wounding luciferase reporter mice of the collagen 1 alpha 2 (Col 1a2) promoter activity. Our data reveal that Col 1a2 starts to be transcribed at D2 after wounding, reaching a plateau after 7 days. Sustained macrophage depletion significantly reduced collagen 1a2 transcription from D4, indicating that the control of fibrosis by macrophages occurs during the early stages of the wound healing process. In conclusion, our results demonstrate an important role of wound macrophages in the control of collagen production during wound healing. DOI

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Section: Discussionmentioning

confidence: 99%

Wound‐associated macrophages control collagen 1α2 transcription during the early stages of skin wound healing

Rodero

Legrand

Bou‐Gharios

et al. 2013

Experimental Dermatology

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“…4V) (Nystrom et al 2013); and periostin (POSTN) and phospholipase C-eta-2 protein (PLCH2), which are mainly expressed in dermal fibroblasts ( Fig. 4X-4Y) (Ontsuka et al 2012). …”

Section: Proteins Expressed In Melanocytes Langerhans Cells and Fibrmentioning

confidence: 99%

Expression of Human Skin-Specific Genes Defined by Transcriptomics and Antibody-Based Profiling

Edqvist

Fagerberg

Hallström

et al. 2014

J Histochem Cytochem.

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To increase our understanding of skin, it is important to define the molecular constituents of the cell types and epidermal layers that signify normal skin. We have combined a genome-wide transcriptomics analysis, using deep sequencing of mRNA from skin biopsies, with immunohistochemistry-based protein profiling to characterize the landscape of gene and protein expression in normal human skin. The transcriptomics and protein expression data of skin were compared to 26 (RNA) and 44 (protein) other normal tissue types. All 20,050 putative protein-coding genes were classified into categories based on patterns of expression. We found that 417 genes showed elevated expression in skin, with 106 genes expressed at least five-fold higher than that in other tissues. The 106 genes categorized as skin enriched encoded for well-known proteins involved in epidermal differentiation and proteins with unknown functions and expression patterns in skin, including the C1orf68 protein, which showed the highest relative enrichment in skin. In conclusion, we have applied a genome-wide analysis to identify the human skin-specific proteome and map the precise localization of the corresponding proteins in different compartments of the skin, to facilitate further functional studies to explore the molecular repertoire of normal skin and to identify biomarkers related to various skin diseases.

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“…Periostin induces proliferation of cardiomyocytes, modulating the healing process of myocardial infarction (13,14). We and others have recently shown that periostin has another physiological role in cutaneous wound repair (15)(16)(17). Furthermore, periostin is involved in the development of various tumors via the integrin/PI3K/Akt pathway (10).…”

Section: Introductionmentioning

confidence: 99%

Periostin promotes chronic allergic inflammation in response to Th2 cytokines

et al. 2012

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Allergic inflammation triggered by exposure of an allergen frequently leads to the onset of chronic inflammatory diseases such as atopic dermatitis (AD) and bronchial asthma. The mechanisms underlying chronicity in allergic inflammation remain unresolved. Periostin, a recently characterized matricellular protein, interacts with several cell surface integrin molecules, providing signals for tissue development and remodeling. Here we show that periostin is a critical mediator for the amplification and persistence of allergic inflammation using a mouse model of skin inflammation. Th2 cytokines IL-4 and IL-13 stimulated fibroblasts to produce periostin, which interacted with α v integrin, a functional periostin receptor on keratinocytes, inducing production of proinflammatory cytokines, which consequently accelerated Th2-type immune responses. Accordingly, inhibition of periostin or α v integrin prevented the development or progression of allergen-induced skin inflammation. Thus, periostin sets up a vicious circle that links Th2-type immune responses to keratinocyte activation and plays a critical role in the amplification and chronicity of allergic skin inflammation.

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Periostin, a matricellular protein, accelerates cutaneous wound repair by activating dermal fibroblasts

Cited by 105 publications

References 55 publications

Wound‐associated macrophages control collagen 1α2 transcription during the early stages of skin wound healing

Wound‐associated macrophages control collagen 1α2 transcription during the early stages of skin wound healing

Expression of Human Skin-Specific Genes Defined by Transcriptomics and Antibody-Based Profiling

Periostin promotes chronic allergic inflammation in response to Th2 cytokines

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