Peripheral blood leukocyte alterations in mice induced by a hemoregulatory pentapeptide (HP 5b)

Lærum, Ole Didrik; Paukovits, Walter R.

doi:10.1016/0145-2126(85)90079-7

Cited by 9 publications

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References 8 publications

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“…Recently, two of us have reported the purification and synthesis of a hernoregulatory pentapeptide associated with mature human granulocytes which acts at several levels of hemopoiesis both in vivo and in vitro (15,16). In addition to a strong inhibitory effect on mye-80 KREJA ET AL lopoiesis in general, leading to reduced numbers of granulocytes in peripheral blood (17), the peptide also reduced the numbers of multipotent stem cells when given in high doses (16). Furthermore, smaller doses inhibiting myelopoiesis led t o a rapidly increased erythropoiesis, while high doses were followed by a reduced erythropoietic cell number as well as reduced erythropoietic proliferative activity in vivo (18,19).…”

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Effects of a hemoregulatory peptide (HP5b) on erythroid and myelopoietic colony formation in vitro

Kreja

Hågå

Müller‐Bérat

et al. 1986

Scandinavian Journal of Haematology

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A hemoregulatory peptide (HPSb) associated with mature human granulocytes has been investigated for inhibitory effects on murine hemopoietic stem cells in vitro. Both highly purified peptide from natural sources and a synthetic analog peptide have been investigated in parallel. Strong inhibitory effects were found on myelopoietic colony formation in the dose range 10-13-10-s mol/l. On exceeding this dose, the inhibitory effect disappeared. Moderate to slight inhibitory effects on erythroid colony formation (BFU-E and CFU-E) from adult animals were only seen in 1000 x the optimal doses for myelopoiesis. No effect was found on CFU-E from fetal liver. The peptide thus has a selective effect on myelopoiesis in a certain dose range. A regulatory mechanism for the peptide on hemopoiesis is proposed.

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mentioning

confidence: 99%

Effects of a hemoregulatory peptide (HP5b) on erythroid and myelopoietic colony formation in vitro

Kreja

Hågå

Müller‐Bérat

et al. 1986

Scandinavian Journal of Haematology

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Selectivity of hemoregulatory peptide (HP5b) action in culture

Lærum

Frostad

Aakvaag

et al. 1990

The International Journal of Cell Cloning

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A synthetic analog of a hemoregulatory peptide associated with mature human granulocytes (HP5b) has been investigated for inhibitory effects on various cell types in culture as compared to inhibitory action on mouse and human myelopoietic colonies (CFU-gm), which occurs from 1 X 10(-13) to 1 X 10(-6) M in vitro. This includes colony formation by lymphoid T and B cells in capillary cultures, as well as mitogen activation of T, B and NK cells. At higher concentrations, i.e., above 1 X 10(-7) M, an inhibitory effect was found on colony formation. Neither the production of interleukin (IL) 3 by mitogen-activated T cells, nor the proliferation of the IL-3-dependent L/B cell line were affected by the peptide up to 1 X 10(-5) M. A slight inhibitory effect was found above 1 X 10(-9) M on mouse 3T3 fibroblasts. A series of malignant cell lines was also tested. No effect was seen between 1 X 10(-11) and 1 X 10(-7) M on human mammary carcinoma cells in culture. On Ehrlich ascites mouse mammary carcinoma cells a 30% inhibition was seen at 10(-6) M. On a human glioblastoma cell line (GaMg) no effect was seen, and on a rat glioma cell line (BT5C) an inhibitory effect was seen at 1 X 10(-7) M and above. No significant inhibition of cell growth was seen on SC1 mouse lymphoma cells from 1 X 10(-9) to 1 X 10(-5) M during 7 days of culture. The investigated normal and malignant cell types in culture were thus not inhibited in very low concentrations which act on CFU-gm. However, a variable inhibitory effect was found at higher concentrations where the inhibition of myelopoiesis was maximal and at concentrations where the inhibition is released. The hemoregulatory peptide thus seems to be a concentration-dependent selective inhibitor of myelopoiesis. The finding that various malignant cells do not respond at lower concentrations supports the possibility of using the peptide as a protector of normal cells during cancer chemotherapy.

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The Neutrophilic Granulocyte

Benestad¹,

Lærum²

1989

Current Topics in Pathology

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Peripheral blood leukocyte alterations in mice induced by a hemoregulatory pentapeptide (HP 5b)

Cited by 9 publications

References 8 publications

Effects of a hemoregulatory peptide (HP5b) on erythroid and myelopoietic colony formation in vitro

Effects of a hemoregulatory peptide (HP5b) on erythroid and myelopoietic colony formation in vitro

Selectivity of hemoregulatory peptide (HP5b) action in culture

The Neutrophilic Granulocyte

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