Peripheral Lymphocyte Subsets as a Prognostic Indicator of Mortality and Morbidity in Healthy Dogs

Watabe, Ai; Hanazono, Kiwamu; Komatsu, Takahiro; Fu, Dah-Renn; Endo, Yuichi; Kadosawa, Tsuyoshi

doi:10.1292/jvms.11-0554

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…It has recently been shown that the ratio of CD4 + /CD8 + provides information about the immune status and further predicts the risk of aging-related diseases and mortality [ 41 ]. In the bovine it was shown that a ratio higher than 2 indicates a proper immune status [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

Tienken¹,

Kersten²,

Frahm³

et al. 2015

Animals

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Simple SummarySeveral biological changes occur during the transition from late pregnancy to early lactation which is associated with a high susceptibility of health disorders. Nicotinic acid, as feed additive, is suggested to balance catabolic metabolism of periparturient dairy cows by attenuating lipolysis and impact production performance. This study provides information of the biological changes occurring around parturition with special emphasis on differences between primiparous and multiparous cows. Present results showed that energy-dense feeding prepartum did not result in metabolic imbalances postpartum in dairy cows which were similar in body condition score. Nicotinic acid supplementation did not reveal any effect.AbstractThe periparturient period is critical according to health, productivity and profitability. As this period is fundamental for the success of the lactation period, the interest in improving periparturient health by dietary supplements increased in recent years. The present study investigated the effects of feeding nicotinic acid (NA) combined with varying dietary energy densities on immunological, hematological and biochemical parameters of periparturient cows differing in parity. Thirty-six multiparous and 20 primiparous dairy cows were enrolled in the study 42 days before expected parturition date until 100 days postpartum with the half of the cows being supplemented with 24 g of NA/d. After parturition a diet with 30% concentrate was fed to all cows which was followed by different concentrate escalation strategies. Dietary NA supplementation was ceased on day 24 postpartum. Dietary NA increased (P = 0.010) serum nicotinamide concentrations (mean of 3.35 ± 1.65 µg/mL), whereas NA could not be detected. Present data emphasize that periparturient cows are faced with major physiological challenges and that both parity-groups have different prerequisites to adapt to those changes irrespective of NA supplementation. The overfeeding of energy to cows which were similar in body condition score had only minor effects on periparturient immune system function and the metabolism of those cows.

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Section: Discussionmentioning

confidence: 99%

Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

Tienken¹,

Kersten²,

Frahm³

et al. 2015

Animals

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“…Many similarities between the dog and other animals have been demonstrated, establishing canine antibody equivalents of CD3, CD5, CD4, CD8 used to label lymphocyte T-cell subsets from peripheral blood (McSweeney et al 1998;Hardy et al 2013). Furthermore, in healthy beagle dogs over 8 years old, where tumour incidence becomes higher, the immune status of CD3, CD4, CD8 and CD21 could predict future morbidity and mortality (Watabe et al 2012). By turn, the discovery of CD34 as an immature haematopoietic cell surface antigen and the production of anti CD34-monoclonal antibodies provided an important tool to study haematologic malignancies (Cobbold et al 1994;McSweeney et al 1998;Ostronoff et al 2008;Williams 2008).…”

Section: Flow Cytometry Peripheral Blood Leukocyte Subsets Long-tementioning

confidence: 99%

“…A minimum of 200 white blood cells was counted by two different pathologists. In flow cytometry we established the number of cells for each subpopulation; using an absolute cell counting (cell number/μl of whole blood), and with the percentage gated, which is the frequency (%) of events in the subpopulation compared with all the acquired events (Watabe et al 2012). …”

Section: Cytological Evaluationmentioning

confidence: 99%

Immunophenotyping pattern characterization in canine blood: towards a clinical application

León¹,

Cruz-López

Fermín

et al. 2016

Acta Vet. Brno

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Immunophenotyping is a widely used method for a precise diagnosis and classification of haematopoietic neoplasia in human beings and also in dogs. The gold standard for cell preparation is density gradient centrifugation of mononuclear cells. Alternatively, another way to separate human leukocytes is carrying out whole blood lysis. The aim of this study was to validate whole blood lysis as an alternative method in clinical veterinary procedures using an immunophenotype panel of leukocytes designed by our group. Flow cytometry study of adult canine leukocytes subset groups, using whole blood lysis or mononuclear cells tested against an array of canine leukocyte antibodies were done. Besides differential white blood cell counts were done. Also immunophenotyping studies in whole blood samples stored at 4 °C for 48 h were performed. The Coefficient Variation values were less than 20%, for most of the comparison. Consistent results were observed in phenotyping canine peripheral blood leukocytes. Stability results indicated that whole blood samples might be stored for 48 h without a significant difference in the data compared to samples processed immediately after blood collection. This study shows that whole blood lysis represents an efficient and quick alternative for canine leukocyte preparation. In addition, samples can be analysed immediately or stored for 48 h without a significant difference between them. This is relevant for veterinary medicine considering the lack of facilities in many laboratories to process samples. Flow cytometry, peripheral blood, leukocyte subsets, long-term stability

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“…Decreased lymphocyte subsets due to aging or speci c diseases (4-7) and immunode ciency due to congenital B-cell depletion (8,9) have been reported. Another study proposed that decreased lymphocyte subsets may be useful as a prognostic indicator of mortality (10). However, lymphocyte subset analysis is not often used clinically in small animal practice.…”

mentioning

confidence: 99%

Clinical Use of Lymphocyte Subset Analysis: As a Prognostic Marker for Dogs with Pyometra

Yokota

Yuki

Fujikake

et al. 2021

Preprint

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BackgroundLymphocyte subset analysis is clinically applied in human medicine. However, lymphocyte subset analysis is rarely used in small animal practice. We hypothesized that lymphocyte subsets analysis was useful in small animal practice as a biomarker for evaluating immune competence and predicting the disease prognosis. Lymphocyte subset analysis was performed prospectively for pyometra, a common disease in dogs, to assess its clinical usefulness in small animal practice.ResultsThis study included 29 dogs diagnosed with pyometra. They were classified into group 1 and group 2 on the basis of clinical course postoperatively. Sixteen dogs were classified in group 1 with no adverse events postoperatively. Thirteen dogs experienced adverse events such as increase in C-reactive protein concentration and white blood cell count, discharge from operation site, and hypoglycemia. These dogs were classified as group 2. Nine dogs were below the reference interval for the lymphocyte subset, eight of which were in group 2. Group 2 included significantly more dogs with lymphocyte subset abnormalities (p = 0.005). In the multivariable logistic regression analysis, only the result of lymphocyte subset analysis was significantly associated with adverse events (p = 0.02, 95% confidence interval = 1.68–192). Most dogs in group 2 were successfully treated.ConclusionsThese results indicate that lymphocyte subset analysis is useful as a prognostic tool for pyometra. Further studies are necessary for evaluating the clinical usefulness of lymphocyte subset analysis in pyometra and other diseases.

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Peripheral Lymphocyte Subsets as a Prognostic Indicator of Mortality and Morbidity in Healthy Dogs

Cited by 5 publications

References 20 publications

Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

Effects of Prepartum Dietary Energy Level and Nicotinic Acid Supplementation on Immunological, Hematological and Biochemical Parameters of Periparturient Dairy Cows Differing in Parity

Immunophenotyping pattern characterization in canine blood: towards a clinical application

Clinical Use of Lymphocyte Subset Analysis: As a Prognostic Marker for Dogs with Pyometra

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