Peripheral nerve regeneration within an asymmetrically porous PLGA/Pluronic F127 nerve guide conduit

Oh, Se Heang; Kim, Jun Ho; Song, Kyu Sang; Jeon, Byeong Hwa; Yoon, Jin hwan; Seo, Tae Beom; Namgung, Uk; Lee, Il Woo; Lee, Jin Ho

doi:10.1016/j.biomaterials.2007.11.036

Cited by 181 publications

(157 citation statements)

References 25 publications

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“…This material was selected based on its hydrophilicity, our ability to carefully control its geometry, even in hydrous conditions, and its promise as a guidance channel. 43 We confirmed that the resultant PLGA nerve guidance had a porous structure (Fig. 3c), which is likely to be permeable to nutrients.…”

Section: Novel Device Features and Characterizationsupporting

confidence: 65%

“…Nerve guides were prepared using a modified phase inversion technique. 43 PLGA (lactic to glycolic acid mol ratio of 75:25, M w = 66,000-107,000; Sigma) was dissolved in tetraglycol (Sigma) at 60°C (10 wt%) and then Pluronic Ò F-127 was added (3 wt%) to increase the hydrophilicity. Alginate hydrogel rods were formed by first dissolving alginate (Sigma) in water (4 wt%) and injecting the solution into 2% CaCl 2 with a syringe (14-gauge needle).…”

Section: Device Fabricationmentioning

confidence: 99%

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A Novel Internal Fixator Device for Peripheral Nerve Regeneration

Chuang

Wilson

Love

et al. 2013

Tissue Engineering Part C: Methods

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Recovery from peripheral nerve damage, especially for a transected nerve, is rarely complete, resulting in impaired motor function, sensory loss, and chronic pain with inappropriate autonomic responses that seriously impair quality of life. In consequence, strategies for enhancing peripheral nerve repair are of high clinical importance. Tension is a key determinant of neuronal growth and function. In vitro and in vivo experiments have shown that moderate levels of imposed tension (strain) can encourage axonal outgrowth; however, few strategies of peripheral nerve repair emphasize the mechanical environment of the injured nerve. Toward the development of more effective nerve regeneration strategies, we demonstrate the design, fabrication, and implementation of a novel, modular nerve-lengthening device, which allows the imposition of moderate tensile loads in parallel with existing scaffold-based tissue engineering strategies for nerve repair. This concept would enable nerve regeneration in two superposed regimes of nerve extension-traditional extension through axonal outgrowth into a scaffold and extension in intact regions of the proximal nerve, such as that occurring during growth or limb-lengthening. Self-sizing silicone nerve cuffs were fabricated to grip nerve stumps without slippage, and nerves were deformed by actuating a telescoping internal fixator. Poly(lactic co-glycolic) acid (PLGA) constructs mounted on the telescoping rods were apposed to the nerve stumps to guide axonal outgrowth. Neuronal cells were exposed to PLGA using direct contact and extract methods, and they exhibited no signs of cytotoxic effects in terms of cell morphology and viability. We confirmed the feasibility of implanting and actuating our device within a sciatic nerve gap and observed axonal outgrowth following device implantation. The successful fabrication and implementation of our device provides a novel method for examining mechanical influences on nerve regeneration.

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Section: Novel Device Features and Characterizationsupporting

confidence: 65%

Section: Device Fabricationmentioning

confidence: 99%

A Novel Internal Fixator Device for Peripheral Nerve Regeneration

Chuang

Wilson

Love

et al. 2013

Tissue Engineering Part C: Methods

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“…Nonionic pluronic surfactants are block copoly m e r s o f p o l y o x y e t h y l e n e s a n d w i c h e d b e t w e e n polyoxypropylene chains [22]. These surfactants have been broadly utilized for biomedical applications including drug delivery, nonfouling, and tissue engineering applications [23][24][25]. Amphiphilic polymers due to hydrophobic component can interact with hydrophobic polymer PLGA (75:25), whereas hydrophilic component imparts hydrophilicity.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

et al. 2015

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Abstract. This study was conducted to develop formulations of hydrocortisone butyrate (HB)-loaded poly(D,L-lactic-co-glycolic acid) nanoparticles (PLGA NP) suspended in thermosensitive gel to improve ocular bioavailability of HB for the treatment of bacterial corneal keratitis. PLGA NP with different surfactants such as polyvinyl alcohol (PVA), pluronic F-108, and chitosan were prepared using oil-in-water (O/W) emulsion evaporation technique. NP were characterized with respect to particle size, entrapment efficiency, polydispersity, drug loading, surface morphology, zeta potential, and crystallinity. In vitro release of HB from NP showed a biphasic release pattern with an initial burst phase followed by a sustained phase. Such burst effect was completely eliminated when nanoparticles were suspended in thermosensitive gels and zero-order release kinetics was observed. In HCEC cell line, chitosanemulsified NP showed the highest cellular uptake efficiency over PVA-and pluronic-emulsified NP (59.09±6.21%, 55.74±6.26%, and 62.54±3.30%, respectively) after 4 h. However, chitosan-emulsified NP indicated significant cytotoxicity of 200 and 500 μg/mL after 48 h, while PVA-and pluronic-emulsified NP exhibited no significant cytotoxicity. PLGA NP dispersed in thermosensitive gels can be considered as a promising drug delivery system for the treatment of anterior eye diseases.

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“…Nerve regeneration has been found to be enhanced by utilizing guidance channels filled with neurotrophic factors, such as GDNF, ciliary nerotrophic factor (CNTF), FGF-2, and NT-3 (Grothe, Haastert, & Jungnickel, 2006;Madduri, Feldman, Tervoort, Papaloizos, & Gander, 2010;Madduri, Papaloizos, & Gander, 2010;Oh et al, 2008;Pfister et al, 2008;Yang et al, 2007). Various types of nerve guides have been developed by blending chitosan with different growth factors (Table 1.8).…”

Section: Chitosan Conduits Combined With Neurotrophic Factors or Neurmentioning

confidence: 99%

The Use of Chitosan-Based Scaffolds to Enhance Regeneration in the Nervous System

Gnavi

Barwig²,

Freier³

et al. 2013

International Review of Neurobiology

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Various biomaterials have been proposed to build up scaffolds for promoting neural repair. Among them, chitosan, a derivative of chitin, has been raising more and more interestamongbasicandclinicalscientists.Anumberofstudieswithneuronalandglial cellcultures haveshownthat thisbiomaterial has biomimetic properties,which make it a good candidate for developing innovative devices for neural repair. Yet, in vivo experimental studies have shown that chitosan can be successfully used to create scaffolds that promote regeneration both in the central and in the peripheral nervous system. In this review, the relevant literature on the use of chitosan in the nervous tissue, either alone or in combination with other components, is overviewed. Altogether, the promising in vitro and in vivo experimental results make it possible to foresee that time for clinicaltrialswithchitosan-basednerveregenerationpromotingdevicesisapproaching quickly.

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Peripheral nerve regeneration within an asymmetrically porous PLGA/Pluronic F127 nerve guide conduit

Cited by 181 publications

References 25 publications

A Novel Internal Fixator Device for Peripheral Nerve Regeneration

A Novel Internal Fixator Device for Peripheral Nerve Regeneration

Nanoparticle-Based Topical Ophthalmic Gel Formulation for Sustained Release of Hydrocortisone Butyrate

The Use of Chitosan-Based Scaffolds to Enhance Regeneration in the Nervous System

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