Periplaneta americana extract promotes intestinal mucosa repair of ulcerative colitis in rat

Abstract

To investigate the mechanism of Periplaneta americana extract promoting intestinal mucosal repair of OXZ-induced colitis in rat. Methods: All experiments used an equal number of male and female SD rats (n=48).We injected OXZ into the colon to induce UC rat model. To determine the optimal concentration of P. Americana's extract (PA-40), it was classified into low (L), medium (M), and high (H) doses. After OXZ treatment, each drug was administered by enema for 7 consecutive days. Rats were divided into the following 6 groups: (1) Saline treatment group (NC), (2) OXZ treatment UC model group (MC), (3) OXZ + budesonide group (BUN), (4) OXZ + PA-40 L group, (5) OXZ + PA-40 M group, (6) OXZ + PA-40 H group. Disease activity index (DAI) scores, colon length, histopathological score, serum cytokine level (IL-4, IL-10, iNOS, tNOS), and amount of MPO, EGF, IL-13 in colonic mucosa were measured. Results: PA treatment had a significant healing effect on the OXZ-colitis model and significantly reduced the lesioned area, especially in the PA-40H groups. PA treatment did not alter the expression of IL-10 and MPO level, but increased EGF (epidermal growth factor) and decrease IL-13 in the colonic tissue. PA inhibited the rise of NOSs (nitric oxide synthase) and decreased the serum IL-4 level. Conclusions: The data suggest that Periplaneta americana extract may be a potential compound for the treatment of colonic lesions. The mechanism may be related to inhibiting the secretion of IL-13 and promoting the formation of EGF.

Treatment of colitis by oral negatively charged nanostructured curcumin in rats

“…Some other experimental studies have been published using plant extracts and drugs for the treatment of UC, emphasizing other mechanisms as inhibiting the secretion of IL-13, increased immunoexpression of NF-kB in rectum, etc. 38 , 39 . The histopathologic and macroscopic validations of the scores used in the present study were based on previous methods 40 .…”

Periplaneta americana Oligosaccharides Exert Anti-Inflammatory Activity through Immunoregulation and Modulation of Gut Microbiota in Acute Colitis Mice Model

“…DSS treatment disrupts intestinal epithelial barrier, inhibits crypt cell proliferation and promotes apoptosis, causing diarrhea, rectal bleeding, body weight loss, hematochezia, colon length shortening, crypt abscess, and inflammation, which resembles human ulcerative colitis [ 5 , 27 ]. P. americana possesses excellent therapeutic effects with a long clinical application history [ 28 , 29 ], such as antitumor, enhanced immunity, antibacterial, anti-inflammatory and analgesic effects, tissue repair [ 30 ] and promoting liver regeneration [ 31 ]. PA-40, an extract of P. americana , has been widely used in the treatment of ulcerative colitis in China, and has achieved good results [ 30 ].…”

“…P. americana possesses excellent therapeutic effects with a long clinical application history [ 28 , 29 ], such as antitumor, enhanced immunity, antibacterial, anti-inflammatory and analgesic effects, tissue repair [ 30 ] and promoting liver regeneration [ 31 ]. PA-40, an extract of P. americana , has been widely used in the treatment of ulcerative colitis in China, and has achieved good results [ 30 ]. Periplanetasin-2 [ 32 ] and periplanetasin-5 peptide [ 33 ], derived from P. americana , exert significant anti-inflammatory activities in Clostridium difficile toxin A induced pseudomembranous colitis mice and lipopolysaccharides (LPS)-induced Raw264.7 macrophage cells, respectively.…”

The Regulating Effect of CII-3 and Its Active Components from Periplaneta americana on M1/M2 Macrophage Polarization

“…Periplaneta americana has been recorded in many authoritative TCM documents, such as Shennong’s Classic of Materia Medica [ 9 ]. With modern drug research, a series of Chinese patent medicines, such as “Kangfuxin Solution”, “Ganlong Capsule”, and “Xinmailong Injection”, were developed for use in the clinical setting [ 10 ].…”