Peritumoral eosinophils predict recurrence in colorectal cancer

Abstract

In colorectal cancer, the presence and extent of eosinophil granulocyte infiltration may render important prognostic information. However, it remains unclear whether an increasing number of eosinophils might simply be linked to the overall inflammatory cell reaction or represent a self-contained, antitumoral mechanism that needs to be documented and promoted therapeutically. Peri-and intratumoral eosinophil counts were retrospectively assessed in 381 primary colorectal cancers from randomly selected patients. Tumors were diagnosed in American Joint Committee on Cancer (AJCC)/Union Internationale Contre le Cancer (UICC) stage I in 21%, stage II in 32%, stage III in 33%, and stage IV in 14%. Presence and extent of eosinophils was related to various histopathological parameters as well as patients' outcome. Overall, peri-and intratumoral eosinophils were observed in 86 and 75% cancer specimens. The peritumoral eosinophil count correlated strongly with the intratumoral eosinophil count (R ¼ 0.69; Po0.001) and with the intensity of the overall inflammatory cell reaction (R ¼ 0.318; Po0.001). Both increasing peri-and intratumoral eosinophil counts were significantly associated with lower T and N classification, better tumor differentiation, absence of vascular invasion, as well as improved progression-free and cancer-specific survival. However, only peritumoral eosinophils, but not intratumoral, were an independent prognosticator of favorable progression-free (hazard ratio 0.75; 95% confidence interval 0.58-0.98; P ¼ 0.04) and cancer-specific survival (hazard ratio 0.7; 95% confidence interval 0.52-0.93; P ¼ 0.01)-independent of the intensity of overall inflammatory cell reaction. This was also found for patients with AJCC/ UICC stage II disease, wherein the presence of peritumoral eosinophils was significantly associated with favorable outcome. In conclusion, the number of peritumoral eosinophils had a significant favorable impact on prognosis of colorectal cancer patients independent of the overall tumor-associated inflammatory response. Evaluation of peritumoral eosinophils represents a promising readily assessable tool and should therefore routinely be commented on in the pathology report.

New Insights Into the Role of Tissue Eosinophils in the Progression of Colorectal Cancer: A Literature Review

“…After scanning the titles and abstracts of the retrieved studies, we selected the full text of 15 original research articles in accordance with the objectives established and all articles were included in our review. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] Data from the included studies were independently extracted from each study and comprised: author; year of publication; sample size; participants' characteristics (mean age and gender proportion); follow-up assessment; tumour classification, stage and clinicopathological features; TATE characterization (i.e. staining, count and location); and major findings.…”

“…[9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] Twelve studies associated TATE with survival and/or metastatic behaviour of CRC 9,11,12,[14][15][16][18][19][20][21][22][23] and three studies associated TATE with malignant progression of colorectal lesions. 10,13,17 The first set of studies evaluated 2523 CRCs.…”

“…10,13,17 The first set of studies evaluated 2523 CRCs. 9,11,12,[14][15][16][18][19][20][21][22][23] The mean age of the participants was recorded in seven studies and ranged between 62.1 and 73 years. 9,11,14,16,[21][22][23] Nine studies detailed gender proportion, totalling 835 males and 1136 females.…”

Molecular genetic and morphological features of malignant neoplasms of the stomach and colon with tissue eosinophilia

“…Для исследова-ния распределения полиморфных вариантов генов р53 (G215C) и р21 (A1026G) проводили выделение дезоксири-бонуклеиновой кислоты из образцов тканей, фиксированных в формалине и залитых в парафин, полученных с границы резекции. Генотипирование образцов дезоксирибонуклеиновой кислоты по полиморфизмам генов осуществляли путём анализа полиморфизма длины рестрикционных фрагментов продуктов амплификации Эозинофильные гранулоциты могут инфильтрировать опухолевую ткань и участвовать в защите организма челове-ка от опухолевой трансформации клеток [1][2][3]. Некоторые авторы отмечают наличие связи тканевой эозинофилии с благопри-ятным прогнозом заболевания и увеличе-нием 5-летней выживаемости пациентов с опухолевой патологией [2,3].…”

“…Генотипирование образцов дезоксирибонуклеиновой кислоты по полиморфизмам генов осуществляли путём анализа полиморфизма длины рестрикционных фрагментов продуктов амплификации Эозинофильные гранулоциты могут инфильтрировать опухолевую ткань и участвовать в защите организма челове-ка от опухолевой трансформации клеток [1][2][3]. Некоторые авторы отмечают наличие связи тканевой эозинофилии с благопри-ятным прогнозом заболевания и увеличе-нием 5-летней выживаемости пациентов с опухолевой патологией [2,3]. Вместе с тем, в литературе есть сведения о способ-ности эозинофилов обеспечивать процесс ремоделирования тканей и, напротив, со-действовать развитию и прогрессированию опухоли [4,5].…”