Perivascular Innervation of the Pulmonary Artery in Human and Swine: A Comparative Study for the Development of an Experimental Model of Denervation

Koshevaya, E; Mitrofanova, Lubov; Гончарова, Н. С.; Leandro, Heber Ivan Condori; Вахрушев, А. Д.; Korobchenko, L; Мурашова, Лада; Галагудза, М. М.; Perminova, A. A.; Моисеева, О. М.; Mikhaylov, Evgeny N.

doi:10.1007/s10517-020-05051-w

Cited by 2 publications

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“…Another issue is RF-induced PA wall injury with subsequent PA remodeling as well as the concomitant damage of parasympathetic nerve fibers that lie together with sympathetic ones [ 21 , 32 ] and mediate the PA vasodilatation effect [ 33 ]. Various types of micro-injuries including PA wall dissection, coagulation of media and adventitia, parietal thrombosis, and hemorrhages in different layers at the sites of RF applications and adjacent areas have been revealed on pathomorphological examination in swine [ 22 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…Mallory trichrome staining (Biovitrum, Russia) was used to assess adventitia and perivascular adipose tissue in the areas of PA ablation. Immunohistochemical labeling was performed with antibodies for S100 protein (Dako Cytomation, Denmark) to detect nerve fibers as previously described [ 21 ]. The Leica Application Suite V 4.5.0 image analyzer and Leica Scope (Leica, Germany) were used for morphometric analysis.…”

Section: Methodsmentioning

confidence: 99%

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Transcatheter radiofrequency pulmonary artery denervation in swine: the evaluation of lesion degree, hemodynamics and pulmonary hypertension inducibility

et al. 2021

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Background Mechanisms of positive effects of pulmonary artery (PA) denervation (PADN) remain poorly understood. The study aimed to evaluate pulmonary hemodynamic changes after PADN and their association with the extent of PA wall damage in an acute thromboxane A2 (TXA2)-induced pulmonary hypertension (PH) model in swine. Methods In this experimental sham-controlled study, 17 normotensive male white Landrace pigs (the mean weight 36.2 ± 4.5 kg) were included and randomly assigned to group I (n = 9)—PH modeling before and after PADN, group II (n = 4)—PADN only, or group III (n = 4)—PH modeling before and after a sham procedure. Radiofrequency (RF) PADN was performed in the PA trunk and at the proximal parts of the right and left PAs. PA wall lesions were characterized at the autopsy study using histological and the immunohistochemical examination. Results In groups I and II, no statistically significant changes in the mean pulmonary arterial pressure nor systemic blood pressure were found after PADN (−0.8 ± 3.4 vs 4.3 ± 8.6 mmHg, P = 0.47; and 6.0 ± 15.9 vs -8.3 ± 7.5 mmHg, P = 0.1; correspondingly). There was a trend towards a lower diastolic pulmonary arterial pressure after PADN in group I when compared with group III during repeat PH induction (34.4 ± 2.9 vs 38.0 ± 0.8; P = 0.06). Despite the presence of severe PA wall damage at the RF application sites, S100 expression was preserved in the majority of PA specimens. The presence of high-grade PA lesions was associated with HR acceleration after PADN (ρ = 0.68, p = 0.03). No significant correlation was found between the grade of PA lesion severity and PA pressure after PADN with or without PH induction. Conclusions Extended PADN does not affect PH induction using TXA2. Significant PA adventitia damage is associated with HR acceleration after PADN. Possible delayed effects of PADN on perivascular nerves and pulmonary hemodynamics require further research in chronic experiments.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%