Permeabilization of Haematococcus pluvialis and solid-liquid extraction of astaxanthin by CO2-based alkyl carbamate ionic liquids

Khoo, Kuan Shiong; Ooi, Chien Wei; Chew, Kit Wayne; Foo, Su Chern; Lim, Jun Wei; Tao, Yang; Jiang, Ning; Ho, Shih Hsin; Show, Pau Loke

doi:10.1016/j.cej.2021.128510

Cited by 61 publications

(19 citation statements)

References 37 publications

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“…The pretreatment with the IL can be considered as a technique to permeabilize the cells, as verified by the change of cell morphology. Some ILs were also applied for cell permeabilization of microbial cells. ,, It further confirms that there may be an effective permeabilization into the inner part of T18 cells to extract the DHA-rich oils by the hydrophobic IL.…”

Section: Resultsmentioning

confidence: 74%

Extraction of a Docosahexaenoic Acid-Rich Oil from Thraustochytrium sp. Using a Hydrophobic Ionic Liquid

Zhang

Ward

Meramo

et al. 2023

ACS Sustainable Chem. Eng.

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Thraustochytrium sp. (T18) is a marine heterokont that exhibits a fast growth rate and accumulates a high proportion of polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA), as essential compounds for health. The extraction of DHA-rich oil from marine microorganisms is challenging due to the difficulty in the substantial cost of lyophilization, the incompatibility of solvents with wet biomass, and the large volumes of organic solvents required by traditional extraction techniques. To address these concerns, ionic liquids (ILs) have been used to improve the oil yield from a wet oleaginous microorganism under mild conditions. In this study, a novel hydrophobic IL, 1-decyl-3-methyl-imidazolium bis(2,4,4-trimethylpentyl)phosphinate ([C10mim][( i C8)2PO2]) was first applied as pretreatment to aid in the extraction of DHA-rich oil from T18 biomass in the aqueous phase. A central composite design and response surface methodology were used to study the effect of different extraction variables on the oil yield (temperature, pH, and the mass ratio of IL to dried T18). The extraction conditions were numerically optimized to maximize the oil yield [69.4 ± 0.5% (w/w)] within the experimental range (the temperature of 76 °C, pH of 5.0, and mass ratio of 4.2). In addition, the extraction efficiency was up to 97.4 ± 0.4% of the total lipid content. The extracted oil contained 44.7 ± 0.2% DHA under 1 h of the pretreatment process. In this work, the proposed approach was found to be efficient and sustainable for the water-compatible recovery of a DHA-rich oil recovery process.

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Section: Resultsmentioning

confidence: 74%

Extraction of a Docosahexaenoic Acid-Rich Oil from Thraustochytrium sp. Using a Hydrophobic Ionic Liquid

Zhang

Ward

Meramo

et al. 2023

ACS Sustainable Chem. Eng.

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“…7 (a) show that the ILs ratios of 9:1 and 8:2 had higher extraction efficiencies. It was suggested that, lipid extraction increases as the ratio increases, and a low ratio of DIMCARB to methanol is not favorable for cell permeabilization [ 10 ]. A study by Miazek et al reported that ionic liquid might easily release lipids from inside the microalgae cells by disorganizing the structure of the fiber bundle in the cell wall [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

“…We used a modified method reported in other studies [ 10 , 11 , 16 ] the recyclability of IL was carried out as follows: IL DIMCARB was recovered from the lipid extract and reused in the new extraction batch. In order to get enough ionic liquid for distillation, the scale of extraction was multiplied by 6.…”

Section: Methodsmentioning

confidence: 99%

“…Using carbon dioxide to prepare these ILs also helps reduce the influence of this greenhouse gas on climate modification. CO 2 -based alkyl carbamate ILs have demonstrated their ability to optimize extraction of bioproducts such as astaxanthin [ 10 ], lutein [ 11 ] and curcumin [ 12 ] from microalgae species. In this study, it was demonstrated that the cell wall could be permeabilized and lipids extracted from Chlorella pyrenoidosa using these ILs.…”

Section: Introductionmentioning

confidence: 99%

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Alkyl carbamate ionic liquids for permeabilization of microalgae biomass to enhance lipid recovery for biodiesel production

Kamdem

Lai²

2023

Heliyon

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“…have a very rigid three layered cell wall structure, as well as a much smaller cell diameter of about 2 μm [ 56 ]. However, Haematococcus pluvialis , with its well-known hydrolysis-resistant outer layer [ 76 ], were highly resistant to ultrasonic treatment that the astaxanthin extractability was only 11.79% after ultrasonic treatment [ 74 ], though the cells generally range from 10 to 20 μm in diameter.…”

Section: Ultrasound Operation Parameters For Effective Cell Disruptionmentioning

confidence: 99%

Ultrasound for microalgal cell disruption and product extraction: A review

Liu

Cui³

et al. 2022

Ultrasonics Sonochemistry

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Permeabilization of Haematococcus pluvialis and solid-liquid extraction of astaxanthin by CO2-based alkyl carbamate ionic liquids

Cited by 61 publications

References 37 publications

Extraction of a Docosahexaenoic Acid-Rich Oil from Thraustochytrium sp. Using a Hydrophobic Ionic Liquid

Extraction of a Docosahexaenoic Acid-Rich Oil from Thraustochytrium sp. Using a Hydrophobic Ionic Liquid

Alkyl carbamate ionic liquids for permeabilization of microalgae biomass to enhance lipid recovery for biodiesel production

Ultrasound for microalgal cell disruption and product extraction: A review

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