Permeabilization of yeast cells (Kluyveromyces lactis) with organic solvents

Flores, Marina; Voget, Claudio Enrique; Ertola, R. J.

doi:10.1016/0141-0229(94)90177-5

Cited by 75 publications

(39 citation statements)

References 21 publications

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“…Different procedures are reported in literature to achieve the contact between β-galactosidase with substrate (Decleire et al, 1987, Flores et al, 1994, Kippert, 1995, but in our experiments better results were obtained by treated cells with ethanol (Lee et al, 2004). The set up of cell treatment was carried out by evaluating the experimental conditions, in particular time effect (from 5 min to 4 h), temperature (from 5 to 20 °C) and ethanol concentration (from 5 to 60% v/v).…”

Section: Preliminary Screeningmentioning

confidence: 99%

Influence of substrate on β-galactosidase production byKluyveromyces strains

et al. 2008

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The aim of the present research was to investigate the influence of culture conditions on the levels of β-galactosidase (EC 3.2.1.23) activity produced by Kluyveromyces strains. Interest was focused on evaluating enzyme activity levels when lactose or cheese whey was employed as substrate in culture medium formulation. From an overall look at the obtained results, the tested strains were found to be able to produce β-galactosidase at promising levels. The use of cheese whey, either for strain maintenance and production trials, allowed to obtain a high cell yield associated with β-galactosidase production. The maximum β-galactosidase volumetric activity, EA max 66.5 IU/ml, corresponding to 3184 EA spec IU/g cell dw, was obtained with K. marxianus MIM 782 at 37 °C and 72 h incubation.

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Section: Preliminary Screeningmentioning

confidence: 99%

Influence of substrate on β-galactosidase production byKluyveromyces strains

et al. 2008

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“…The outer wall of yeast cells can be permeabilized by a large variety of chemical compounds including surfactant (Laouar, Mulligan, & Lowe, 1992), detergents (Abraham, & Bhat, 2008), or solvents (Flores, Voget, & Ertola, 1994). Yeast cells were permeabilized by a cationic, non-toxic and biodegradable detergent N-Lauroyl sarcosine (LS), a 350-fold increase in catalase activity and an enhancement of 5'-nucleotides in the supernatant was obtained (Flores, Voget, & Ertola, 1994).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Yeast cell disruption strategies for recovery of intracellular bio-active compounds — A review

Liú

Ding

Sun

et al. 2016

Innovative Food Science & Emerging Technologies

116

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“…Toluene permeabilization of cells has been shown to be an economical, easy, convenient, and safe process for enzymatic bioconversion and product formation (Cá novas et al 2005;Flores et al 1994). Time-course reactions of both toluene-treated and untreated cells were investigated under optimal conditions (Fig.…”

Section: D-psicose Production By Toluene-treated and Untreated Cells mentioning

confidence: 99%

d-Psicose production from d-fructose using an isolated strain, Sinorhizobium sp.

Kim

et al. 2006

World J Microbiol Biotechnol

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Sinorhizobium sp., which can convert D-fructose into D-psicose, was isolated from soil. The optimal pH, temperature, and cell concentration for D-psicose production with the isolated strain were 8.5, 40°C, and 60 mg/ml, respectively. The toluene-treated cells showed 2.5-and 4.8-fold increases in the D-psicose concentration and productivity compared with untreated washed cells. Under the optimal conditions, the toluene-treated cells produced 37 g D-psicose/l from 70% (w/v) (3.9 M) D-fructose after 15 h.

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Permeabilization of yeast cells (Kluyveromyces lactis) with organic solvents

Cited by 75 publications

References 21 publications

Influence of substrate on β-galactosidase production byKluyveromyces strains

Influence of substrate on β-galactosidase production byKluyveromyces strains

Yeast cell disruption strategies for recovery of intracellular bio-active compounds — A review

d-Psicose production from d-fructose using an isolated strain, Sinorhizobium sp.

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