Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia × hybrida microshoots

Kotis, M.; Yupsanis, Traianos; Syros, T.; Economou, A. S.

doi:10.1007/s10535-009-0096-x

Cited by 9 publications

(6 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in agreement with reports that; the propagation of cuttings is dependent on the concentrations and application methods of exogenous rooting hormones (Carter and Slee, 1993;Maile and Nieuwenhuis, 1996). Overall mean survival rate was computed based on each of the concentrations of IBA used and 2000 ppm of IBA yielded the highest survival rate (i.e 43.39%) followed by the control (37.86%), 3000 ppm (36.52%) and then lastly 4000 ppm (31.70%) which goes agrees with findings by that exogenous auxins are vital for rooting capacity as well as survival of cuttings (Kotis et al, 2009).…”

Section: Discussionsupporting

confidence: 84%

Effect of Different Levels of I.B.A Concentration on Clonal Propagation of Eucalyptus Species in Ghana West Africa

Sarpong¹,

Agyemang²,

Siaw³

et al. 2020

Trop.cal.Agr

View full text Add to dashboard Cite

Effective rooting and survival of cuttings has become more important owing to increased global wood demands and climate change. This study assesses the survival of ten (10) clones each of Eucalyptus grandis x Eucalyptus urophylla (E. grandis x E. urophylla) and Eucalyptus pellita (E. pellita) clonal cuttings when treated with different Indole-3-butyric acid (IBA) auxin concentrations of 0 ppm (control), 2000 ppm, 3000 ppm and 4000 ppm respectively. Overall, the mean survival of cuttings with respect to both species (10 clones each) was low (i.e < 50%) for all concentrations of IBA used. E. grandis x E. urophylla cuttings had the highest mean survival (43.39%) when treated with 2000 ppm of IBA and lowest mean survival (37.86%) when treated with 0 ppm. E. pellita (P) cuttings had the highest mean survival (27.14%) when treated with 2000 ppm of IBA. Individual clones however showed higher survival with respect to IBA treatments. Eucalyptus grandis x Eucalyptus urophylla clone (GH11) achieved a mean survival rate of 75% with 2000ppm of IBA. E. pellita clone P2 achieved its highest survival (52.68%) in the control experiment (0 ppm). Although IBA application had significant effect on cutting survival, parent material (individual clones) also plays a vital role in cutting survival as some clones such P51 and P82 achieved survivals of less than 10% given all four levels of IBA used. Individual clones also respond to IBA concentration differently. GH3 and GH10 had their highest survivals (i.e 66.07% and 61.61% respectively) in the control (0ppm of IBA). Results from this study can be used in plus tree clonal selection regimes to identify the best economically rewarding clones in terms of cutting survival to be used in forest plantation establishments.

show abstract

Section: Discussionsupporting

confidence: 84%

Effect of Different Levels of I.B.A Concentration on Clonal Propagation of Eucalyptus Species in Ghana West Africa

Sarpong¹,

Agyemang²,

Siaw³

et al. 2020

Trop.cal.Agr

View full text Add to dashboard Cite

show abstract

“…This is in agreement with (Carter and Slee, 1993;Maile and Nieuwenhuis, 1996) reports that; the propagation of cuttings is dependent on the concentrations and application methods of exogenous rooting hormones. Overall mean survival rate was computed based on each of the concentrations of IBA used and 2000 ppm of IBA yielded the highest survival rate (i.e 43.39%) followed by the control (37.86%), 3000 ppm (36.52%) and then lastly 4000 ppm (31.70%) which goes agrees with ndings by (Kotis et al, 2009) that exogenous auxins are vital for rooting capacity as well as survival of cuttings.…”

Section: Discussionsupporting

confidence: 81%

“…Exogenous auxins are vital in the determination of rooting capacity, whereas most commercial propagation is done by rooting with IBA (Kotis et al, 2009). Global mean productivity of Eucalyptus clonal plantations has in the last 30 years increased from 25-30 m 3 /ha/year to 35-45 m 3 /ha/year with exceptional cases where productivity exceeds 60 m 3 /ha/year (Rezende et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Levels of I.B.A Concentration on Clonal Propagation of Eucalyptus Species in Ghana West Africa.

Sarpong

Agyemang

Siaw

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundIncreasing global wood demands have necessitated an overall push for cost-effective methods of vegetative propagation using cuttings. Vegetative plant propagation through cuttings although a breakthrough is mostly hampered with the inability of cuttings to root effectively to achieve maximum yield. Ten clones each of Eucalyptus grandis x Eucalyptus urophylla (E. grandis x E. urophylla) and Eucalyptus pellita (E. pellita) were treated with Indole-3-butyric acid (IBA) concentrations of 0 ppm (control), 2000 ppm, 3000 ppm and 4000 ppm respectively.ResultsOverall mean survival of cuttings was low (< 50%) for all concentrations of IBA used in this research. Survival rates of individual clones however varied from clone to clone. E. grandis x E. urophylla cuttings had the highest mean survival (43.39%) when treated with 2000 ppm of IBA and lowest mean survival (37.86%) when treated with 0 ppm. Results from the survival rates of E. grandis × E. Urophylla and Eucalyptus pellita cuttings were subjected to an Analysis of Variance test to identify any significant differences between hormone concentration and cutting survival. ANOVA results indicated that differences between cutting–IBA concentrations for E. grandis × E. Urophylla were significant (p=1.85x10-08) which is lower than standard p value (p=0.05) used in the test.For E. pellita (P) cuttings the highest mean survival (27.14%) was achieved when treated with 2000 ppm of Indole-3-butyric acid (IBA). Individual clones however showed significant differences in survival. E. pellita clone P2 achieved the highest survival (52.68%) in the control experiment (0 ppm). Some clones i.e P51 and P82 achieved survivals of less than 10% in all four levels of IBAConclusionThe best surviving Eucalyptus grandis x Eucalyptus urophylla clone (GH11) achieved a mean survival rate of 75% when the GH11 basal cutting was treated with 2000 ppm IBA. The results indicated that the production of clones with survival rates of less than 20% should be discarded since it makes no economic sense clones with survival rates of more than 40% should be planted out in the field to compare field performance with greenhouse survival.

show abstract

“…1I). H 2 O 2 has a role in rhizogenesis and other differentiation processes (Molassiotis et al 2004, Kotis et al 2009, Kärk and Koutaniemi 2010, Lu et al 2013 as well as in constituting an essential part of the defense system (Keyes et al 2001(Keyes et al , 2007. H 2 O 2 is also produced by parasitic plants at the site of host/parasite attachment (Keyes et al 2007).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora

Salcedo-Morales¹,

Jiménez–Aparicio²,

Cruz-Sosa³

et al. 2014

Biologia plant.

View full text Add to dashboard Cite

In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 µM catechin, 25 µM vanillin, or 25 µM H 2 O 2 . Of the treatments tested, 25 µM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H 2 O 2 and flavonoids.

show abstract

Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia × hybrida microshoots

Cited by 9 publications

References 59 publications

Effect of Different Levels of I.B.A Concentration on Clonal Propagation of Eucalyptus Species in Ghana West Africa

Effect of Different Levels of I.B.A Concentration on Clonal Propagation of Eucalyptus Species in Ghana West Africa

Effect of Different Levels of I.B.A Concentration on Clonal Propagation of Eucalyptus Species in Ghana West Africa.

Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora

Contact Info

Product

Resources

About