Peroxidation of Body Fat in Vitamin E Deflciency

Abstract

Summary. Chicks fed vitamin E deficient diets containing 5 per cent cod liver oil developed peroxides in the adipose tissue during the period in which the known symptoms of exudation, and particularly brown coloration of the same tissue occurred. The relation between the peroxidation and the symptoms is discussed. Subcutaneous injection of 0.3 ml of cod liver oil with a peroxide value of 16 to chicks receiving a normal diet, resulted in very marked peroxidation at the site of injection accompanied by a progressive deposition of yellow to brown fibrous material. This local peroxidation was not prevented by the incorporation of 2.4 mg of free natural alpha‐tocopherols in the injected oil. Rats reared on vitamin E deficient diets containing 20 per cent cod liver oil from weaning developed peroxides in the adipose tissue preceding the visible yellow‐brown coloration which appeared first in the paraepididymal fat, then in the intraperitoneal, and finally in the subcutaneous fat. When females together with their offspring were given the same diet from the time of delivery, the symptoms in the young developed earlier than in weanling rats and were more marked. The yellow‐brown color of the adipose tissue is due to two substances, one of which is extractable with fat solvents, while the other, which occurs in larger quantity, is soluble in dilute alkali. A further, preliminary, characterization of this latter substance is given. Vitamin E has no influence on the lowering of hemoglobin caused by feeding high cod liver oil diets to young rats.

The Effect of Selenium in the Hen’s Diet on Egg Production, Hatchability, Performance of Progeny and Selenium Concentration in Eggs

“…Examination of embryos from GSHpx activity, no effect on the incidence hens fed the basal diet, that died between of exudative diathesis would be expected. day 18 and day 21 of incubation, revealed Experiment 2 was conducted to evaluate that approximately 25% showed symptoms the effect of selenium supplementation on resembling the classical signs of exudative diathesis in growing chicks, as described by Dam and Glavind (1938) and Dam and Granados (1945). Furthermore, approximately 50% of the newly-hatched chicks showed signs of the deficiency disease.…”

The history of selenium—vitamin E interrelationships

“…Thus Dam,Kruse,Prange & Ssndergaard (195 I ) found that methylene blue, thionine, thiodiphenylamine, tetraethyl thiuram disulphide (Antabuse), nordihydroguiaretic acid and ascorbic acid could all afford considerable, but incomplete, protection against the effects of vitamin E deficiency in chicks. Dam & Granados (1952) found that methylene blue could even prevent resorptions in rats. Moore, Sharman & Ward (1953a,b,c, 1954 failed to confirm this last finding.…”

“…T h e incisor teeth of rats lost their normal brown pigmentation (Moore, 1943). In rats given cod-liver oil, but not dosed with vitamin E, the fat depots became brown and peroxides were present (Dam & Granados, 1945). Red blood cells taken from rats deficient in vitamin E were found to be rapidly haemolysed in the presence of dialuric acid (Gyorgy & Rose, 1948 One further lesion in the rat deserves our special attention in view of its importance in studies of Se-vitamin E interrelationships.…”

Vitamins E and A

“…However, with the possible exception of adipose tissue (Dam and Granados, 1945), no increase in tissue peroxides could be detected in experimental animals made vitamin E deficient (Dam, 1962;Bunyan et a/., 1967). However, with the possible exception of adipose tissue (Dam and Granados, 1945), no increase in tissue peroxides could be detected in experimental animals made vitamin E deficient (Dam, 1962;Bunyan et a/., 1967).…”