Peroxidative and radiation damage to isolated lysosomes

Desai, I. D.; Sawant, P. L.; Tappel, Al L.

doi:10.1016/0304-4165(64)90054-6

Cited by 151 publications

(26 citation statements)

References 13 publications

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“…Oxidation of fatty acids in membranes, especially of lysosomes, had been proposed as a mechanism for aging in animal systems (11,25,26). Besides directly destroying lipids and altering their ability to function in the membrane, the autoxidation of fatty acids produces highly reactive free radical intermediates (12,25).…”

Section: Resultsmentioning

confidence: 99%

On the Mechanism of Aging in Soybean Seeds

Parrish¹,

Leopold²

1978

Plant Physiol.

130

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Changes in seeds of soybeans (Glycine max IL.I Meff. var. Wayne) which occur during accelerated aging (41 C, 100% relative humidity) showed subsequent loss of vigor, a decline in early respiratory activity, increased leakage of electrolytes, losses of as much as 10% dry weight from imbibing cotyledons, and a decrease in the swelling response of the imbibing system (seed plus H20). Each of these changes with aging is interpreted as resulting from deteriorative changes in membranes.The performance capability of seeds in general and soybeans in particular declines during storage. This aging or loss of vigor is evidenced by delayed germination and emergence, slower growth, increased susceptibility to environmental stresses, and, ultimately, a decline in germinability (8,13,18,29 (29), degradation of the respiratory system (2), loss of ATP production/storage capacity (6, 9), and deterioration of membranes (4,7,23,28).The rate at which seeds lose vigor during storage is affected by environmental factors such as temperature, moisture, and 02/CO2 concentrations (13,16,28 For measurements of vigor, 50 seeds were placed in rolled paper toweling, wetted with distilled H20, and held in the dark for 4 days at 25 ± 1 C with occasional additions of distilled H20. The number of seeds germinated (embryonic axis longer than I cm), average root length, and axis weight (measured by removing cotyledons and weighing the remaining root and hypocotyl) were recorded.Respiration was determined manometrically by adding I ml of H20 to six cotyledons (0.5 g) (20). Leakage of electrolytes from imbibing cotyledons was determined by placing six cotyledons in 10 ml of distilled H20 and measuring the changes in electrical conductivity of the H20 with a conductivity bridge (20,24).Changes in the total volume of an imbibing system (seed plus H20) were measured using a dilatometer (20): 100 g of dry seedswere placed in a 1-liter flask; H20 was added to completely fill the flask, which was then stoppered. A burette was inserted through the stopper so that the H20 rose into the graduated portion ofthe burette. Changes in the total volume ofthe imbibing system could then be observed as changes in the H20 level in the burette. RESULTSThree seed performance parameters, per cent germination, root length, and axis weight, were used to measure changing seed vigor with accelerated aging (Table I). Germination began to decline after 4 days of accelerated aging, but axis growth in terms of both weight and root length showed adverse effects after 3 days of treatment. The seeds of this seed lot were completely nongerminable after 7 days of accelerated aging.Changes in respiratory activity following accelerated aging are shown in Figure 1. Manometric determinations ofearly respiration are complicated by nonrespiratory exchange of gases (desorption) and vapor equilibration during the first several min after the introduction of H20 (20); for this reason, comparisons of respiratory activity were begun after 45 min of imbibition. The data in Figure I indicat...

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Section: Resultsmentioning

confidence: 99%

On the Mechanism of Aging in Soybean Seeds

Parrish¹,

Leopold²

1978

Plant Physiol.

130

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“…T h e accumulation of these cells together with the increase in vascular permeability may be induced by UV light through various nonimmune mechanisms involving changes of cell membrane permeability (13), liberation of vasoactive amines from tissue reservoirs (14) and disruption of lysosomes of pre-existing and accumulating cells (15,16) with liberation of enzymatic activities and leukotactic factors (1 7).…”

Section: Discussionmentioning

confidence: 99%

Effect of complement and polymorphonuclear leukocyte depletion on experimental skin lesions resembling systemic lupus erythematosus

Natali¹,

Mottolese²,

Nicotra³

1975

Arthritis & Rheumatism

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Immunopathologic cutaneous lesions resembling human systemic lupus erythematosus (SLE) can be induced in mice sensitized to ultraviolet (UV)-irradiated DNA following whole body irradiation with UV light. The lesions are characterized by the formation of immune complexes at different skin sites. The role played by cellular and humoral mediators in the pathogenesis of this experimental model was investigated. The results obtained suggest that inflammation that follows UV radiation is the major factor responsible for this pathology. Accordingly mice that were rendered neutrophil (PMN) deficient did not manifest skin lesions, and depletion of C3 complement component left them unchanged. I n addition time course studies showed that PMN depletion did not prevent a delayed skin involvement. Thus multiple factors seem to mediate the onset of the immunopathologic changes previously described. chemically altered DNA molecules are able to induce a specific immune response (1,2).Native DNA irradiated with ultraviolet (UV) light (UV-DNA) is highly immunogenic (3) and has been used in experimental animal models to elucidate some of the immunologic events leading to kidney (4) and skin (5) involvement in systemic lupus erythematows in humans. Cutaneous lesions in particular could be induced with high frequency in mice with circulating antibodies to UV-DNA following exposure to UV light. This treatment has been shown to be capable of inducing formation of the UV-DNA photoproduct, thymidine dimer (6), and of releasing it from the skin into the blood stream (7).The skin immunopathology closely resembled human lesions in that in vivo fixation of mouse 7-globulins and C3 complement component to epidermal cell nuclei and dermal-epidermal junction could be observed together with accumulation of inflammatory cells. In order to investigate the role played by cellular and humoral mediators in the formation of these lesions, the effect of polymorpholeukocyte (PMN) and complement (C3) depletion on the appearance of skin immunopathology was investigated. Results of experiments reported here showed that PMN accumulation in the skin, which follows UV light irradiation, is indispensable for the onset of the cutaneous pathology. C3 depletion prevented only in vivo binding of the complement component to the skin sites where mouse y-globulins were bound to UV-DNA antigen.

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“…Lipid peroxide content in the serum was assayed by thiobarbituric acid procedure. The byproducts of lipid peroxidation (Aldehyde especially malonic dialdehyde) formed by degradation of hydroperoxide react with thiobarbituric acid forming pink coloured trimethine complex which was measured spectrophotometrically at 530nm (Desai et al, 1964).…”

Section: Biochemical Estimationmentioning

confidence: 99%

Preliminary Studies on Alloxan Induced Anti-Diabetic Activity of Some Siddha Medicinal Plants in Male Albino Rats.

Elango¹

2017

WJPPS

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Siddha traditional system of medicine, a science that uses herbal medicines extensively, originated in India. The present studies focus the anti-diabetic activity in Boerhavia diffusa, Momordica charantia and Carica papaya leaves; have been reported to be beneficial for treating type 2 diabetes induced by alloxan. Diabetes mellitus is one of the common metabolic disorders acquiring around 2.8% of the world's population and is anticipated to cross 5.4% by the year 2025. Since long back herbal medicines have been the highly esteemed source of medicine, there were many plant and plants phyto-constituents were used for anti diabetic activity. In this preliminary experimental study blood glucose, glycogen, cholesterol and the antioxidant activity (LPO) were studied and statistical analyses were carried out.

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Peroxidative and radiation damage to isolated lysosomes

Cited by 151 publications

References 13 publications

On the Mechanism of Aging in Soybean Seeds

On the Mechanism of Aging in Soybean Seeds

Effect of complement and polymorphonuclear leukocyte depletion on experimental skin lesions resembling systemic lupus erythematosus

Preliminary Studies on Alloxan Induced Anti-Diabetic Activity of Some Siddha Medicinal Plants in Male Albino Rats.

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