Peroxisome Proliferator-Activated Receptor-γ Gene: A Key Regulator of Adipocyte Differentiation in Chickens

Abstract

The peroxisome proliferator-activated receptors (PPAR) are members of the nuclear hormone receptor superfamily. Peroxisome proliferator-activated receptor-gamma is regarded as a "master regulator" of adipocyte differentiation in mammals. The current study was designed to investigate the function and regulatory mechanism of PPARgamma in chicken adipogenesis by RNA interference. Preadipocytes were isolated from the abdominal fat tissue of 12-d-old chickens and cultured. Small-interference PPARgamma RNA (siPPARgamma) was synthesized by in vitro transcription and transfected into chicken preadipocytes by using liposomes. The suppressive effect of siPPARgamma was detected by real-time reverse-transcription PCR and reverse-transcription PCR. The results showed that transient transfection with siPPARgamma significantly inhibited differentiation and enhanced proliferation of chicken preadipocytes (P < 0.05). The adipogenesis-associated adipocyte fatty acid-binding protein gene was down-regulated when PPARgamma was silenced. The current work indicates that PPARgamma is a key regulator of chicken preadipocyte differentiation.

Expression and functional analyses of Krüppel-like factor 3 in chicken adipose tissue

“…[14][15][16][17] In the current study, we found that gKLF3 overexpression suppressed chicken C/EBPα promoter activity in DF1 cells and chicken preadipocytes (p < 0.01; Fig. 5).…”

“…Effects of gKLF3 overexpression on promoter activities of important genes in adipose tissue C/EBPα and PPARγ are two master positive regulators of adipogenesis in mammals and birds. [14][15][16][17] We investigated the effects of gKLF3 overexpression on the promoter activities of C/EBPα and PPARγ using luciferase reporter assay. As shown in Fig.…”

FSH stimulates lipid biosynthesis in chicken adipose tissue by upregulating the expression of its receptor FSHR

“…Starting on day 0, chicken FSH (Zhonghao Biological Technology Co., LTD, Beijing, China) was included in the media at concentrations of 0, 5, 10, or 20 mIU/ml. Every 2 days, cell morphology, proliferation ( 29 ), and lipid content ( 30 ) were determined in triplicate wells from each treatment. Total RNA was also prepared from three wells and stored for q-PCR and microarray analysis.…”

Effects of caponization on growth, carcass, and meat characteristics and the mRNA expression of genes related to lipid metabolism in roosters of a Chinese indigenous breed

“…Further, the increased LDL concentration indicated that large amounts of fat are transported out of the liver through VLDL and hydrolyzed by LPL, as remnants of hydrolyzed VLDL are found in the LDL form (Hermier 1997). We also found increased expression of PPARγ, which is a key regulator of chicken preadipocyte differentiation (Wang et al 2008). The up-regulated mRNA level of PPARγ in capon adipose tissues implied that caponization enhanced adipocyte differentiation.…”

“…In particular, PPARγ is a key regulator of chicken preadipocyte differentiation (Wang et al 2008); its expression increases with differentiation of adipocytes, and reaches a peak when adipocytes get mature (Fan et al 2011).…”