Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells

Corpas, Francisco J.; Barroso, Juan B.; Rı́o, Luis A. del

doi:10.1016/s1360-1385(01)01898-2

Cited by 458 publications

(284 citation statements)

References 51 publications

Supporting

Mentioning

270

Contrasting

Unclassified

Order By: Relevance

“…The generation of superoxide radicals has also been reported in the matrices and membranes of peroxisomes (Ló pez-Huertas et al, 1999;del Río et al, 2006). All these findings point to the important role played by peroxisomes in the cellular metabolism of reactive oxygen species (Corpas et al, 2001del Río et al, 2006).…”

mentioning

confidence: 74%

“…] debate (Corpas et al, 2009b). On the basis of previous experimental data, we had hypothesized that pea leaf peroxisomes could be a potential source of NO in plant cells (Corpas et al, 2001). This study therefore aims to demonstrate the presence of NO in the peroxisomes of other plant species (using Arabidopsis as a model for plant analysis), to determine whether peroxisomal NO is released into the cytosol and to analyze its physiological function.…”

Section: Discussionmentioning

confidence: 99%

“…Peroxisomes are single membrane-bound organelles whose basic enzymatic constituents are catalase and H 2 O 2 -producing flavin oxidases as their basic enzymatic and are found in virtually all eukaryotic cell types (Corpas et al, 2001;Hayashi and Nishimura, 2006;Reumann et al, 2007;Pracharoenwattana and Smith, 2008;Palma et al, 2009). These oxidative organelles are characterized by metabolic plasticity, as their enzymatic content can vary according to the organism, cell/tissue type, and environmental conditions (Mullen et al, 2001;Hayashi and Nishimura, 2003;Corpas et al, 2009a).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis Plants

et al. 2009

View full text Add to dashboard Cite

Peroxisomes are unique organelles involved in multiple cellular metabolic pathways. Nitric oxide (NO) is a free radical active in many physiological functions under normal and stress conditions. Using Arabidopsis (Arabidopsis thaliana) wild type and mutants expressing green fluorescent protein through the addition of peroxisomal targeting signal 1 (PTS1), which enables peroxisomes to be visualized in vivo, this study analyzes the temporal and cell distribution of NO during the development of 3-, 5-, 8-, and 11-d-old Arabidopsis seedlings and shows that Arabidopsis peroxisomes accumulate NO in vivo. Pharmacological analyses using nitric oxide synthase (NOS) inhibitors detected the presence of putative calcium-dependent NOS activity. Furthermore, peroxins Pex12 and Pex13 appear to be involved in transporting the putative NOS protein to peroxisomes, since pex12 and pex13 mutants, which are defective in PTS1-and PTS2-dependent protein transport to peroxisomes, registered lower NO content. Additionally, we show that under salinity stress (100 mM NaCl), peroxisomes are required for NO accumulation in the cytosol, thereby participating in the generation of peroxynitrite (ONOO 2 ) and in increasing protein tyrosine nitration, which is a marker of nitrosative stress.

show abstract

mentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis Plants

et al. 2009

View full text Add to dashboard Cite

show abstract

“…4). Besides, peroxisomes, which are well known to be involved in H 2 O 2 breakdown through the action of CAT, have recently been found to be an important source of ROS (Corpas et al, 2001), while accumulation of ROS in mitochondria has been found to play a crucial role in programmed cell death (Maxwell et al, 2002;Foyer and Noctor, 2005). In response to herbivores, H 2 O 2 levels are likely to be elevated as long as the attacks persist.…”

Section: Discussionmentioning

confidence: 99%

Effects of Feeding Spodoptera littoralis on Lima Bean Leaves. III. Membrane Depolarization and Involvement of Hydrogen Peroxide

et al. 2006

View full text Add to dashboard Cite

In response to herbivore (Spodoptera littoralis) attack, lima bean (Phaseolus lunatus) leaves produced hydrogen peroxide (H 2 O 2 ) in concentrations that were higher when compared to mechanically damaged (MD) leaves. Cellular and subcellular localization analyses revealed that H 2 O 2 was mainly localized in MD and herbivore-wounded (HW) zones and spread throughout the veins and tissues. Preferentially, H 2 O 2 was found in cell walls of spongy and mesophyll cells facing intercellular spaces, even though confocal laser scanning microscopy analyses also revealed the presence of H 2 O 2 in mitochondria/peroxisomes. Increased gene and enzyme activations of superoxide dismutase after HW were in agreement with confocal laser scanning microscopy data. After MD, additional application of H 2 O 2 prompted a transient transmembrane potential (V m ) depolarization, with a V m depolarization rate that was higher when compared to HW leaves. In transgenic soybean (Glycine max) suspension cells expressing the Ca 21 -sensing aequorin system, increasing amounts of added H 2 O 2 correlated with a higher cytosolic calcium ([Ca 21 ] cyt ) concentration. In MD and HW leaves, H 2 O 2 also triggered the increase of [Ca 21 ] cyt , but MD-elicited [Ca 21 ] cyt increase was more pronounced when compared to HW leaves after addition of exogenous H 2 O 2 . The results clearly indicate that V m depolarization caused by HW makes the membrane potential more positive and reduces the ability of lima bean leaves to react to signaling molecules.

show abstract

“…Peroxisomes are involved in various catabolic and anabolic pathways such as the photorespiration cycle, fatty acid ␤-oxidation, the glyoxalate cycle, ureide metabolism, and the generation of cellular messengers like superoxide radicals and nitric oxide (Tabak et al, 1999;Corpas et al, 2001). Unlike chloroplasts and mitochondria, peroxisomes do not possess their own DNA.…”

mentioning

confidence: 99%

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

2002

View full text Add to dashboard Cite

Peroxisomes were visualized in living plant cells using a yellow fluorescent protein tagged with a peroxisomal targeting signal consisting of the SKL motif. Simultaneous visualization of peroxisomes and microfilaments/microtubules was accomplished in onion (Allium cepa) epidermal cells transiently expressing the yellow fluorescent protein-peroxi construct, a green fluorescent protein-mTalin construct that labels filamentous-actin filaments, and a green fluorescent proteinmicrotubule-binding domain construct that labels microtubules. The covisualization of peroxisomes and cytoskeletal elements revealed that, contrary to the reports from animal cells, peroxisomes in plants appear to associate with actin filaments and not microtubules. That peroxisome movement is actin based was shown by pharmacological studies. For this analysis we used onion epidermal cells and various cell types of Arabidopsis including trichomes, root hairs, and root cortex cells exhibiting different modes of growth. In transient onion epidermis assay and in transgenic Arabidopsis plants, an interference with the actin cytoskeleton resulted in progressive loss of saltatory movement followed by the aggregation and a complete cessation of peroxisome motility within 30 min of drug application. Microtubule depolymerization or stabilization had no effect.

show abstract

Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells

Cited by 458 publications

References 51 publications

Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis Plants

Peroxisomes Are Required for in Vivo Nitric Oxide Accumulation in the Cytosol following Salinity Stress of Arabidopsis Plants

Effects of Feeding Spodoptera littoralis on Lima Bean Leaves. III. Membrane Depolarization and Involvement of Hydrogen Peroxide

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

Contact Info

Product

Resources

About