Peroxy-radical-mediated chemiluminescence: mechanistic diversity and fundamentals for antioxidant assay

Fedorova, G. F.; Trofimov, A. V.; Vasil’ev, R. F.; Veprintsev, Timur L.

doi:10.3998/ark.5550190.0008.815

Cited by 17 publications

(8 citation statements)

References 179 publications

(342 reference statements)

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“…Additionally, it is observed that 1,4-benzoquinone suppresses ROS at high concentrations (>10 −3 g L −1 ). This effect was attributed earlier [76] to its ability to form dioxetanes, which is typical for unsaturated hydrocarbons [77]. Figure 6 presents the ROS content in the enzyme system at different concentrations of F10-12.…”

Section: Shows Maximal Values Of T Relsupporting

confidence: 66%

Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents

Kovel

Kicheeva

Vnukova

et al. 2021

IJMS

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Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.

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Section: Shows Maximal Values Of T Relsupporting

confidence: 66%

Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents

Kovel

Kicheeva

Vnukova

et al. 2021

IJMS

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“…In the past, various types of chemical and physical probes were used to enhance the photon emission [31] , [32] , [33] , [34] . Chemical probes are capable to enter in the lipid reaction chain and form excited state by its interaction with the product of lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

Linoleic Acid-Induced Ultra-Weak Photon Emission from Chlamydomonas reinhardtii as a Tool for Monitoring of Lipid Peroxidation in the Cell Membranes

Prasad

Pospı́šil

2011

PLoS ONE

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Reactive oxygen species formed as a response to various abiotic and biotic stresses cause an oxidative damage of cellular component such are lipids, proteins and nucleic acids. Lipid peroxidation is considered as one of the major processes responsible for the oxidative damage of the polyunsaturated fatty acid in the cell membranes. Various methods such as a loss of polyunsaturated fatty acids, amount of the primary and the secondary products are used to monitor the level of lipid peroxidation. To investigate the use of ultra-weak photon emission as a non-invasive tool for monitoring of lipid peroxidation, the involvement of lipid peroxidation in ultra-weak photon emission was studied in the unicellular green alga Chlamydomonas reinhardtii. Lipid peroxidation initiated by addition of exogenous linoleic acid to the cells was monitored by ultra-weak photon emission measured with the employment of highly sensitive charged couple device camera and photomultiplier tube. It was found that the addition of linoleic acid to the cells significantly increased the ultra-weak photon emission that correlates with the accumulation of lipid peroxidation product as measured using thiobarbituric acid assay. Scavenging of hydroxyl radical by mannitol, inhibition of intrinsic lipoxygenase by catechol and removal of molecular oxygen considerably suppressed ultra-weak photon emission measured after the addition of linoleic acid. The photon emission dominated at the red region of the spectrum with emission maximum at 680 nm. These observations reveal that the oxidation of linoleic acid by hydroxyl radical and intrinsic lipoxygenase results in the ultra-weak photon emission. Electronically excited species such as excited triplet carbonyls are the likely candidates for the primary excited species formed during the lipid peroxidation, whereas chlorophylls are the final emitters of photons. We propose here that the ultra-weak photon emission can be used as a non-invasive tool for the detection of lipid peroxidation in the cell membranes.

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“…Singlet oxygen, a non-radical, is an excited state of O 2 , which is not very reactive in its ground state (Equation 3). The chlorophyll and their precursors performed singlet oxygen's primary production (Krieger-Liszkay, 2005;Tripathy and Oelmüller, 2012 (Fedorova et al, 2007). The spectrum range of singlet-and triplet-excited pigments belongs to green-red (550-750 nm) and red-near IR (750-1000 nm), respectively (Pospíšil et al, 2014;Sauermann et al, 1999).…”

Section: Antioxidant Defense Mechanismsmentioning

confidence: 99%

Ultra-weak photon emission: a nondestructive detection tool for food quality and safety assessment

Nematollahi

Alinasab

Nassiri

et al. 2020

qas

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A new aspect covering interactions between cells and their surroundings via electromagnetic waves was introduced by applying ultra-weak photon emission (UPE). The UPE originates from the relaxation of electronically excited species resulting from oxidative metabolic processes and oxidative stress associated with reactive oxygen species (ROS). The ROS plays a critical role in the quality of foods, and their determination is of extreme importance. The ROS and the intensity of the UPE have significantly correlated. The UPE can be effectively monitored by specific instruments such as photomultiplier tube and charged-coupled devices. The current review is devoted to providing an overview of the quality of food products by the aid of UPE via evaluating the correlations between UPE and food quality indices. In this regard, the UPE can be utilized in food quality as a real-time, noninvasive, and nondestructive technique without complex instruments. However, the implementation of the UPE method for evaluation of food quality needs further investigations.

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Peroxy-radical-mediated chemiluminescence: mechanistic diversity and fundamentals for antioxidant assay

Cited by 17 publications

References 179 publications

Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents

Toxicity and Antioxidant Activity of Fullerenol C60,70 with Low Number of Oxygen Substituents

Linoleic Acid-Induced Ultra-Weak Photon Emission from Chlamydomonas reinhardtii as a Tool for Monitoring of Lipid Peroxidation in the Cell Membranes

Ultra-weak photon emission: a nondestructive detection tool for food quality and safety assessment

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