Peroxynitrite, a product between nitric oxide and superoxide anion, plays a cytotoxic role in the development of post-bypass systemic inflammatory response☆

Hayashi, Yoshitaka; Sawa, Yoshiki; Nishimura, Motonobu; Fukuyama, Naoto; Ichikawa, Hajime; Ohtake, Shigeaki; Nakazawa, Hiroe; Matsuda, Hikaru

doi:10.1016/j.ejcts.2004.03.033

Cited by 45 publications

(29 citation statements)

References 26 publications

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“…The mechanisms underlying this observation may involve even greater scavenging of NO by increased oxidative stress. Diabetes 33 and CPB 34 have been shown to increase vascular formation of peroxynitrite. It is well established that peroxynitrite avidly oxidises the eNOS cofactor tetrahydrobiopterin (BH 4 ) to form cofactor inactive molecules such as dihydrobiopterin.…”

Section: Discussionmentioning

confidence: 99%

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Guillou

Tamion

Jouet

et al. 2012

Diabetes and Vascular Disease Research

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Background: Diabetes is a risk factor for perioperative complications after cardiac surgery. We studied its effects on mesenteric endothelial function in a cardiopulmonary bypass (CPB) model. Methods: Forty Wistar rats were divided into four groups: sham (D-CPB-), cardiopulmonary bypass (D-CPB+), diabetic (D+CPB-) and diabetic that have undergone CPB (D+CPB+). Two samples of mesenteric artery were used for nitric oxide synthase (NOS) Western blot analysis, and two others for assessing contractile response and endothelium relaxations. Nitrite products and tumour necrosis factor-alpha (TNF-α) were assessed as markers of inflammatory response. Results: We observed an enhanced contractile response to the α-adrenergic agonist associated with impairment of mesenteric vasorelaxation in D+CPB+ rats. Western immunoblot analysis of D+CPB+ highlighted an additive effect of hyper-expression of inducible NOS. A significantly increased inflammatory response was observed after CPB in diabetic animals. Conclusions: This work confirms the potential deleterious impact of diabetes on the mesenteric endothelium during CPB in cardiac surgery.

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Section: Discussionmentioning

confidence: 99%

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Guillou

Tamion

Jouet

et al. 2012

Diabetes and Vascular Disease Research

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“…On the contrary, NO can be deleterious, when reacts with superoxide anion to form peroxynitrite, under the impact of factors that enhance the increase in inflammatory and oxidative stress status ( Fig. 1) [13][14][15]. In general, peroxynitrite induces cell damage by lipid peroxidation as well as impairment of the normal function of enzymes and proteins via nitration and oxidation [13].…”

Section: Introductionmentioning

confidence: 96%

Conflicting effects of nitric oxide and oxidative stress in chronic heart failure: potential therapeutic strategies

Tousoulis

Papageorgiou²,

Briasoulis³

et al. 2011

Heart Fail Rev

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Chronic heart failure (CHF) is characterized by decreased nitric oxide (NO) bioavailability. In addition, the beneficial NO turns to be deleterious when it reacts with superoxide anion, leading to peroxynitrite formation. Numerous experimental and clinical studies have reported increased production of reactive oxygen species (superoxide, hydrogen peroxide, hydroxyl radical) both in animals and patients with CHF. Moreover, there are indicative data suggesting mechanisms associated with endothelial dysfunction in states of CHF, mainly attributed to decreased NO bioavailability and enhanced inactivation of the latter. Thus, such molecules appear to be potential targets in patients with CHF. These patients are strong candidates to receive a variety of therapeutic agents, some of which have known antioxidant effects. Classic treatment with statins or angiotensin converting enzyme inhibitors has been found to be beneficial in restoring NO and improving myocardial function and structure. Other agents such as sildenafil and b-blockers along with novel agents such as NO synthase transcription enhancers have been proved to be also beneficial, but their use for such a purpose is still controversial. Approaches using more-effective antioxidants or targeting myocardial oxidant-producing enzymes and oxidative or nitrosative stress might be promising strategies in the future.

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“…• NO (Hayashi et al 2004). In vivo, ONOO -is mostly trapped by substrates, such as thiols or metaloproteins through a bimolecular pathway, and, only its minor fraction is quickly converted into "radical-like" reactive species (Balavoine and Geletii 1999).…”

Section: Anti-oxidative Systemsmentioning

confidence: 99%

Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation

Hrabárová¹,

Juránek²,

Šoltés³

2011

gpb

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Abstract. Current understanding on the role of peroxynitrite in etiology and pathogenesis of some human diseases, such as cardio-vascular diseases, stroke, cancer, inflammation, neurodegenerative disorders, diabetes mellitus and diabetic complications has recently led to intensive investigation of peroxynitrite involvement in physiology and pathophysiology.Mechanism of cytotoxic effects of peroxynitrite involve its reactions with lipids, DNA/RNA, proteins, and polysaccharides, thus triggering cellular responses ranging from subtle changes of cell functioning to severe oxidative damage of the affected macromolecules leading to necrosis or apoptosis. The present work is aimed at providing a brief overview of i) peroxynitrite biosynthesis and reaction pathways in vivo, ii) its synthetic preparation in vitro, and iii) to reveal its potential damaging role in vivo, on actions studied via monitoring in vitro hyaluronan degradation. The complex biochemical behavior of peroxynitrite is determined by a number of variables, such as chemistry of the reaction itself, depending mostly on the involvement of conformational structures of different energy states, concentration of the species involved, content of reactive intermediates and trace transition metal ions, contribution of carbon dioxide, presence of trace organics, and by the reaction kinetics. Recently, in vitro studies of oxidative cleavage of hyaluronan have, in fact, been the subject of growing interest. Here we also describe our experimental set-up for studying peroxynitrite-mediated degradation of hyaluronan, a system, which may be suitable for testing prospective pharmacological substances.

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Peroxynitrite, a product between nitric oxide and superoxide anion, plays a cytotoxic role in the development of post-bypass systemic inflammatory response☆

Abstract: These results suggest that ONOO- plays a crucial role in the development of post-bypass systemic inflammatory response and the pretreatment with quercetin has a potential benefit to avoid deleterious effects of ONOO-.

Cited by 45 publications

References 26 publications

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Conflicting effects of nitric oxide and oxidative stress in chronic heart failure: potential therapeutic strategies

Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation

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Peroxynitrite, a product between nitric oxide and superoxide anion, plays a cytotoxic role in the development of post-bypass systemic inflammatory response☆

Abstract: These results suggest that ONOO- plays a crucial role in the development of post-bypass systemic inflammatory response and the pretreatment with quercetin has a potential benefit to avoid deleterious effects of ONOO-.

Cited by 45 publications

References 26 publications

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Mesenteric endothelial dysfunction in a cardiopulmonary bypass rat model: The effect of diabetes

Conflicting effects of nitric oxide and oxidative stress in chronic heart failure: potential therapeutic strategies

Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation

Contact Info

Product

Resources

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Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation