Peroxyoxalate Chemiluminescence Assay of Hydrogen Peroxide and Glucose Using 2,4,6,8-Tetrathiomorpholuiopyrimido[5,4-d]-pyrimidine as a Fluorescent Component

Abstract

Peroxyoxalate chemiluminescence (CL) assay of hydrogen peroxide (H2O2) or glucose was developed by using 2, 4,6,8-tetrathiomorpholinopyrimido[5,4-d]pyrimidine as a fluorescent component and bis(2,4,6-trichlorophenyl)oxalate (TCPO) as an oxalate. Linear relationships between CL intensity and final concentration of H2O2 from 10.8 to 10-4 M were obtained. The detection limit at the ratio of CL intensities for sample and blank (S/ B) of 3 was 10 nM. The precision for five replicate measurements at 10-5 and 10-6 M of H2O2 were 17.6 and 15.7% of relative standard deviations, respectively. a-D-Glucose was transformed to /3-D-glucose with mutarotase and converted to H2O2 and D-gluconic acid with glucose oxidase, which was detected by using peroxyoxalate CL reaction. A linear calibration graph was obtained up to 1.5X10-4 M of glucose solution. The method was applied to the assay of glucose in human serum. The recovery was 98.2% (n=4). The method correlated well with the conventional colorimetric method (r=0.968).

A MgO Nanoparticles Composite Matrix‐Based Electrochemical Biosensor for Hydrogen Peroxide with High Sensitivity

“…Comparing to other analytical techniques such as spectrometry [2], titrimetry [3], and chemiluminescence [4], electrochemical enzyme biosensors [5 -7] based on redox proteins or enzymes modified electrodes possess unique advantages such as simplicity, high selectivity and intrinsic sensitivity. Horseradish peroxidase (HRP), a heme enzyme, is generally used as a representative to investigate the dynamic and thermodynamic mechanism of the enzyme reactions and develop biosensors for the determination of H 2 O 2 or related compounds.…”

Analytical Studies on the Development of High-Performance Liquid Chromatographic Methods with Fluorescence or Chemiluminescence Detections and Their Practical Applications

“…38,39 By using this combination, sensitive determination of hydrogen peroxide was achieved with an LOD of 10 nM at a signal-toblank ratio of 3. 40 Furthermore, substrates for oxidase such as glucose, uric acid, 41 mono-acetyl polyamines and polyamines 42 could be determined. The method was based on the enzyme reaction to generate hydrogen peroxide, which was performed on-line using an immobilized enzyme column reactor.…”

Synthetic Analytical Studies on Organic Reagents for Biomaterials

“…PO-CL assay of hydrogen peroxide or glucose was developed by using TMP and TCPO. 43 a-D-Glucose was transformed to ,3-D-glucose with mutarotase and converted to H2O2 and D-gluconic acid with glucose oxidase, which was detected by using PO-CL reaction. This method was applied to the assay of glucose in human serum.…”