Persistence and
            <i>In Vivo</i>
            Evolution of Vaginal Bacterial Strains over a Multiyear Time Period

France, Michael; Ma, Bing

doi:10.1128/msystems.00893-22

Cited by 8 publications

(11 citation statements)

References 23 publications

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“…Future studies could analyse sub-CSTs, but this would greatly increase the number of possible transitions, thereby raising an acute statistical challenge (here we already had to collapse CSTs I, II, and V to introduce covariates into the model). In the same vein, analysing metagenomics data could provide additional valuable insights on vaginal microbiota long-term dynamics [21]. For example, for women who appear to have stable CSTs, we could identify the proportion of lineage replacements.…”

Section: Discussionmentioning

confidence: 99%

Factors shaping vaginal microbiota long-term community dynamics in young adult women

Kamiya,

Tessandier,

Elie

et al. 2024

Preprint

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The vaginal microbiota is structured into five main community state types (CST) that are known to affect women's health. CST shifts can occur in less than a day, but there is a lack of follow-ups lasting more than a few months; a gap in the knowledge that hampers our understanding of long-term dynamics. Analysing a longitudinal cohort of 125 women followed for a median duration of 10 months, we show that `optimal' (CST I, II, and V) or `sub-optimal' CST III are more stable in time than the `non-optimal' CST IV. We also find that some probabilities of shifting from one CST to another are associated with covariates such reported number of sexual partners or alcohol consumption. Finally, we simulate population-level consequences of variations in behaviours. In addition to providing one of the first insights on vaginal microbiota dynamics over a year, along with a robust methodological analysis, these results open new perspectives to improve our mechanistic understanding of microbial interactions in the vaginal environment and develop new therapeutic strategies.

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Section: Discussionmentioning

confidence: 99%

Factors shaping vaginal microbiota long-term community dynamics in young adult women

Kamiya,

Tessandier,

Elie

et al. 2024

Preprint

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“…may require persistence at low levels for many years. A recent study provides evidence of persistence of several vaginal bacterial strains in reproductive age women for more than a year (France et al, 2022b).…”

Section: Discussionmentioning

confidence: 99%

Vaginal microbiota of adolescents and their mothers: A preliminary study of vertical transmission and persistence

Bassis¹,

Bullock²,

Sack³

et al. 2023

Front. Microbiomes

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IntroductionFactors that influence vaginal microbiota composition, including its source, are not well understood.MethodsTo determine if vaginal microbiota transmission from mother to daughter at birth could possibly influence the human vaginal microbiota composition in adolescence, we conducted a preliminary study to investigate the relationship between the vaginal microbiota of 13 adolescents (ages 15-21) and their mothers and the daughter’s birth mode.Results and discussionBased on analysis of bacterial 16S rRNA gene sequences, the vaginal microbiotas of mother/daughter pairs were more similar to each other if the daughter was born by vaginal delivery rather than by C-section. Specifically, within pair β-diversity, measured by the Yue and Clayton θ (θYC) distance metric, was significantly lower if the daughter was born by vaginal delivery. Additionally, genome sequences from an important member of the vaginal microbiota, Lactobacillus crispatus, isolated from one mother/daughter pair in which the daughter was born by vaginal delivery, were highly similar based on recombination-filtered single nucleotide polymorphisms (SNPs). Both community-level analysis and isolate genome sequence analysis are consistent with birth-mode dependent transmission and persistence of at least some members of the vaginal microbiota.

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“…For each lawn isolate, optical density of mid exponential phase cells was measured. Roughly 2.5 x 10 7 cells (adjusted to 0.5 x 10 7 for S. aureus to offset higher growth of this species) were added to 50 mL conical tubes along with 35 mL of molten soft TSA. Tubes were mixed by inverting 4 times, then poured into Nunc OmniTrays (Thermo Scientific 242811).…”

Section: Methodsmentioning

confidence: 99%

“…Tubes were mixed by inverting 4 times, then poured into Nunc OmniTrays (Thermo Scientific 242811). Plates were allowed to cool for up to 2 h. While plates cooled, spot cultures were prepared by normalizing the OD of late log-phase sub-cultures to OD 0.1 (roughly 6 x 10 7 CFU/mL) in fresh TSB. A 96-well pipettor (Avidien MicroPro300) was used to dispense 2 µL of spot culture onto each plate.…”

Section: Methodsmentioning

confidence: 99%

Intraspecies warfare restricts strain coexistence in human skin microbiomes

Mancuso,

Baker,

et al. 2024

Preprint

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Determining why only a fraction of encountered or applied bacterial strains engraft in a given person's microbiome is crucial for understanding and engineering these communities. Previous work has established that metabolism can determine colonization success in vivo, but relevance of bacterial warfare in preventing engraftment has been less explored. Here, we demonstrate that intraspecies warfare presents a significant barrier to strain transmission in the skin microbiome by profiling 14,884 pairwise interactions between Staphylococcus epidermidis cultured from eighteen human subjects from six families. We find that intraspecies antagonisms are abundant; these interactions are mechanistically diverse, independent of the relatedness between strains, and consistent with rapid evolution via horizontal gene transfer. Ability to antagonize more strains is associated with reaching a higher fraction of the on-person S. epidermidis community. Moreover, antagonisms are significantly depleted among strains residing on the same person relative to random assemblages. Two notable exceptions, in which bacteria evolved to become sensitive to antimicrobials found on the same host, are explained by mutations that provide phage resistance, contextualizing the importance of warfare among other lethal selective pressures. Taken together, our results emphasize that accounting for intraspecies bacterial warfare is essential to the design of long-lasting probiotic therapeutics.

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Persistence and In Vivo Evolution of Vaginal Bacterial Strains over a Multiyear Time Period

Abstract: The stability of strains within the vaginal microbiota is largely uncharacterized. Should these strains be capable of persisting for extended periods of time, they could evolve within their host in response to selective pressures exerted by the host or by other members of the community.

Cited by 8 publications

References 23 publications

Factors shaping vaginal microbiota long-term community dynamics in young adult women

Factors shaping vaginal microbiota long-term community dynamics in young adult women

Vaginal microbiota of adolescents and their mothers: A preliminary study of vertical transmission and persistence

Intraspecies warfare restricts strain coexistence in human skin microbiomes

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