Persistence of Bacteroidales and other fecal indicator bacteria on inanimated materials, melon and tomato at various storage conditions

Ordaz, Gilberto; Merino-Mascorro, José Ángel; García, Santos; Heredia, Norma

doi:10.1016/j.ijfoodmicro.2019.03.015

Cited by 15 publications

(10 citation statements)

References 36 publications

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“…Our previous studies [ 13 , 24 ] demonstrated that BFG bacteria are not completely eliminated in WWTPs during the activated sludge process. Feng et al [ 55 ] and Ordaz et al [ 56 ] argued that, similarly to E. coli and E. faecalis , Bacteroides species should be regarded as fecal indicator bacteria to accurately describe environmental contamination with human feces. The present findings suggest that markers specific to BFG, in particular HF183/BacR287, are not only as effective as the standard indicators of fecal contamination, but also support accurate identification of the sources of human-caused pollution, which validates the first research hypothesis.…”

Section: Resultsmentioning

confidence: 99%

Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Niestępski

Harnisz

Korzeniewska

et al. 2020

IJERPH

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The aim of this study was to evaluate the applicability of markers specific to Bacteroides fragilis group (BFG) bacteria as indicators of anthropogenic pollution of surface waters. In addition, the impact of wastewater treatment plants (WWTPs) on the spread of genes specific to fecal indicator bacteria and genes encoding antimicrobial resistance in water bodies was also determined. Samples of hospital wastewater (HWW), untreated wastewater (UWW), and treated wastewater (TWW) evacuated from a WWTP were collected, and samples of river water were taken upstream (URW) and downstream (DRW) from the wastewater discharge point to determine, by qPCR, the presence of genes specific to BFG, Escherichia coli and Enterococcus faecalis, and the abundance of 11 antibiotic resistance genes (ARGs) and two integrase genes. The total number of bacterial cells (TCN) in the examined samples was determined by fluorescence in situ hybridization (FISH). Genes specific to BFG predominated among the analyzed indicator microorganisms in HWW, and their copy numbers were similar to those of genes specific to E. coli and E. faecalis in the remaining samples. The abundance of genes specific to BFG was highly correlated with the abundance of genes characteristic of E. coli and E. faecalis, all analyzed ARGs and intI genes. The results of this study indicate that genes specific to BFG can be used in analyses of human fecal pollution, and as indicators of environmental contamination with ARGs. A significant increase in the copy numbers of genes specific to BFG, E. coli, and seven out of the 11 analyzed ARGs was noted in samples of river water collected downstream from the wastewater discharge point, which suggests that WWTPs are an important source of these genes in riparian environments.

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Section: Resultsmentioning

confidence: 99%

Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Niestępski

Harnisz

Korzeniewska

et al. 2020

IJERPH

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“…This may be related to river water dilution and most of the farmers were used natural rainwater for their cultivation. The occurrence of such indicator microorganisms is an indication of the contamination of the vegetables with fecal matter derived from humans and other animals (Cornish, Mensah, & Ghesquire, 1999;Korajkic, McMinn, & Harwood, 2018;Ordaz, Merino-Mascorro, García, & Heredia, 2019).…”

Section: Bacteriological Quality Indicator Organisms Including Aerobic Plate Count Total Coliform and Fecalmentioning

confidence: 99%

Microbial Risk Assessment of Vegetables Irrigated with Akaki River Water in Addis Ababa.

Mengesha

Asfaw

Kidane

et al. 2021

Preprint

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Background: Consumption of vegetable plays important role in human health. Above 60% of Addis Ababa, vegetable demand is covered by smallholder farmers who grow various vegetables by using the Akaki River as their main water source for irrigation. However, applying faecally contaminated water for irrigation could expose fresh vegetable consumers to many health problems. Therefore, this study aimed to evaluate the microbial contamination of irrigation water and freshly produced vegetables. The study assessed the quality of Akaki river water and vegetables. Fresh vegetables including Ethiopian Kale, Lettuce, Cabbage, and Spinach were collected from 14 farmlands, irrigated with Akaki River water, in the dry and wet seasons. Using standard methods, analysis of E.coli, Non-E.coli, Total coliform, fecal coliform, and Total Aerobic Plate Count were done among the Akaki River water and vegetables. Result: The finding of the study indicated that all fresh vegetables were contaminated with total coliforms, fecal coliforms, and Total aerobic in the dry and wet seasons. The overall mean count of E. coli and Non-E. Coli from water samples were 2.09 and >3.48 log10 CFU 10 mL−1. The mean count of TC, FC, and TAC on sampled vegetables were 3.22, 1.37, and 4.72 in the dry season, and 3.87, 2.57, and 5.09 log10 CFU per gram in the wet season, respectively.Conclusion: The Akaki River has been contaminated with non-E. Coli & E. coli coliforms and did not meet the WHO guideline criteria for safe irrigation. The microbial contamination of vegetables was found high and exceeded the HACCPTQM and ICMSF limit values for safe consumption. This calls for farmers and the consumer has to insist on properly processed/stored/sliced fresh vegetables needs to be aware.

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“…Taking these limitations into account, future research can be conducted by keeping the moisture of the surfaces constant throughout the experiment. For example, with the use of a bioclimatic chamber, Ordaz et al (2019) observed a faster inactivation of E. coli inoculated on cellulose filters and on flat cork coupons for 25 days at 25 ˚C and 65% relative humidity (5.8 log CFU and 1.8 log CFU, respectively) than at 10 ˚C and 95% relative humidity (2.9 log CFU and 1.2 log CFU, respectively). Furthermore, the effect of porosity was investigated and the data, presented in Table 4.1, suggest that, apart from the differences between concrete and pavement blocks due to the indirect effect of porosity on surface moisture, as discussed above, there was no direct relation between porosity and E. coli concentration.…”

Section: Flood Cycle On Different Surfacesmentioning

confidence: 99%

Assessment of the Fate of Surrogates for Enteric Pathogens Resulting from the Surcharging of Combined Sewer Systems

Scoullos¹

2021

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καὶ ἐὰν εἰδῶ τὰ μυστήρια πάντα καὶ πᾶσαν τὴν γνῶσιν, ἀγάπην δὲ μὴ ἔχω, οὐδέν εἰμι To my parents vii ACKNOWLEDGMENTSThe completion of this thesis has been an exciting and wonderful journey that I travelled together with many people to whom I am extremely grateful. The gradual deepening into mechanisms taking place in nature, for the benefit of society and the environment, is perhaps the most rewarding experience for the time, resources and energy invested.First of all, my research and this PhD thesis would not be possible without the valuable Scholarship from the Academy of Athens from the Vasiliki Bekiari-Vekri Bequest and a Research Grant from the Alexander S. Onassis Public Benefit Foundation. I am deeply grateful for this opportunity to deepen my knowledge and to strengthen my commitment in finding practical solutions for a sustainable future. I would like to thank my promotor, Professor Dr. Damir Brdjanovic for giving me the opportunity to do a PhD and for his overall supervision, guidance and support since the first day I arrived in Delft. I am very thankful for his important expertise and contribution in formulating this very interesting topic and during all the stages towards the completion of my thesis. Thank you very much! Second, I would like to thank my mentor Dr. Carlos Lopez Vazquez for his day to day supervision, guidance and countless hours of meetings. Carlos, you know in detail all the stages of my PhD research, all the challenges I met, how I managed to overcome them and how I have changed. Thank you for everything! I would like to equally thank Dr. Jack van de Vossenberg for mentoring me in the second half of my PhD. Thank you, Jack, for your invaluable ideas and support! In difficult times you helped me see things in a positive way and you helped me believe in my skills. I would also like to thank Dr. Michael Hammond, who mentored me in the first half of my research. Thank you for your support, your ideas and your encouragement. Sabita, my amazing MSc student, I would like to add you in this list just after my mentors, because although you were my student I learned a lot from you. Your contribution in the research, your self-motivation, your willingness to learn and your smile even in times of desperation motivated me too and I am grateful that I had the opportunity to work with you.I would like to thank Prof. Maria Kennedy for her important input as member of my proposal defence committee and all the reviewers of my PhD proposal, my papers and my thesis.

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Persistence of Bacteroidales and other fecal indicator bacteria on inanimated materials, melon and tomato at various storage conditions

Cited by 15 publications

References 36 publications

Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Markers Specific to Bacteroides fragilis Group Bacteria as Indicators of Anthropogenic Pollution of Surface Waters

Microbial Risk Assessment of Vegetables Irrigated with Akaki River Water in Addis Ababa.

Assessment of the Fate of Surrogates for Enteric Pathogens Resulting from the Surcharging of Combined Sewer Systems

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