Persistence of chromosomal lesions and induced in mouse bone marrow cells by mitomycin C, as evaluated by SCE analysis

Russo, Antonella; Dorigo, E.; Stocco, A.; Majone, Franca

doi:10.1016/0027-5107(93)90020-g

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…These results coincide well with those obtained by other authors e.g. Moore et al (1995), Amer et al (1996) for induction of chromosome aberrations in the spleen; Das and Roy (1990) and SjOblom et al (1995) for the induction of chromosome aberrations in spermatocyte cells and Kram et al (1979) and Russo et al (1993) for the induction of SCE's.…”

Section: Seed Treatmentsupporting

confidence: 93%

Cytogenetic Effect of the Organophosphorous Insecticide DDVP and Its Residues in Stored Faba Beans in Somatic and Germ Cells of the Mouse.

2000

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Section: Seed Treatmentsupporting

confidence: 93%

Cytogenetic Effect of the Organophosphorous Insecticide DDVP and Its Residues in Stored Faba Beans in Somatic and Germ Cells of the Mouse.

2000

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“…had no significant effect on SCEs compared with the control non-treated value, whereas the higher doses of 5.7 , which is known to be a strong SCE-inducing agent. 30 In this respect it should be mentioned that Latt et al 31 reported a small but statistically significant increase in SCE frequencies that may not be indicative of meaningful biological effect but may reflect inherent variability in the system used. However, much is known about the ability of metallic compounds to induce chromosomal aberrations in bone marrow cells of laboratory animals.…”

Section: In Vivo Studiesmentioning

confidence: 97%

In vivo and in vitro studies on the genotoxicity of cadmium chloride in mice

Fahmy¹,

Aly²

2000

J. Appl. Toxicol.

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The genotoxic effect of cadmium chloride was evaluated in chromosomes of experimental mice using in vivo and in vitro studies. In vivo the induction of micronuclei, sister chromatid exchange in mouse bone marrow and chromosomal aberrations in both somatic and germ cells was investigated. Doses 1.9, 5.7 and 7.6 mg kg ؊1 body wt. (single i.p. treatment) induced a significant and dose-dependent increase in the percentage of polychromatic erythrocytes with micronuclei. Such a percentage reached 2.1% with the highest tested dose, compared with 0.57% for the control (non-treated) and 2.2% for mitomycin c as the positive control. The dose of 1.9 mg kg ؊1 body wt. had no significant effect with respect to sister chromatid exchange (SCE) but the doses of 5.7 and 7.6 mg kg ؊1 body wt. increased the frequency of SCEs significantly. The frequency of SCE reached 7.35 ؎ 0.26 per cell after treatment with the highest tested dose, which is a less than twofold increase compared with the control frequency of 4.6 ؎ 0.42 per cell. However mitomycin c induced a much higher effect (12.1 ؎ 0.73). Cadmium chloride also induced a significant increase in the percentage of chromosomal aberrations in mouse bone marrow at the doses of 5.7 and 9.5 mg kg ؊1 body wt. (single i.p. treatment). The effect is a function of cadmium chloride concentration. Moreover, cadmium chloride induced its maximum effect concerning the induction of chromosomal aberrations in mouse bone marrow cells 24 h after treatment, compared with 12 and 48 h.In germ cells, chromosomal aberrations were observed in mouse spermatocytes 12 days post-treatment with the dose of 5.7 mg kg ؊1 body wt. Moreover, a pronounced reduction in the number of spermatocytes was observed after administration of cadmium chloride (0.9, 1.9 and 5.7 mg kg ؊1 body wt.)In in vitro studies, the three tested concentrations of 10, 15 and 20 g ml ؊1 cadmium chloride induced a statistically significant increase in the frequency of SCEs in cultured mouse spleen cells. The concentrations of 15 and 20 g ml ؊1 also induced chromosomal aberrations in mouse spleen culture.The ability of vitamin C (l-ascorbic acid) to minimize the incidence of chromosomal aberrations induced by cadmium chloride in cultured mouse spleen cells was investigated. Vitamin C at the concentrations of 3 and 6 g ml ؊1 significantly minimized the percentage of aberrant cells induced by cadmium chloride. P Ͻ 0.05 level; **significant at P Ͻ 0.01 level (t-test).

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“…treatment with different concentrations of N-nitrosodiethanolamine mutagenic (Carrano et al 1978). Mitomycin C is one of the most powerful inducers of SCE's (Russo et al 1993). The high incidence of SCE's that induced after treatment with NDELA may reflect its action on the cellular DNA repair process.…”

Section: Chemicalsmentioning

confidence: 99%

Effect of Vitamin C against the Genotoxicity Induced, by N-Nitrosodiethanolamine in Mice in vivo Test System.

Fahmy

Aly

2000

CYTOLOGIA

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Persistence of chromosomal lesions and induced in mouse bone marrow cells by mitomycin C, as evaluated by SCE analysis

Cited by 10 publications

References 20 publications

Cytogenetic Effect of the Organophosphorous Insecticide DDVP and Its Residues in Stored Faba Beans in Somatic and Germ Cells of the Mouse.

Cytogenetic Effect of the Organophosphorous Insecticide DDVP and Its Residues in Stored Faba Beans in Somatic and Germ Cells of the Mouse.

In vivo and in vitro studies on the genotoxicity of cadmium chloride in mice

Effect of Vitamin C against the Genotoxicity Induced, by N-Nitrosodiethanolamine in Mice in vivo Test System.

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