Persistence of chromosome aberrations following acute radiation: I, PAINT translocations, dicentrics, rings, fragments, and insertions

Tucker, James D.; Cofield, Jackie; Matsumoto, Kyomu; Ramsey, Marilyn J.; Freeman, D. Carl

doi:10.1002/em.20090

Cited by 32 publications

(20 citation statements)

References 31 publications

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“…It has been definitively demonstrated that in the absence of mitogenic stimulation, cells bearing unstable aberrations are preferentially eliminated via apoptosis (Bassi et al 2003;Belloni et al 2008a), though it is unknown whether this also applies to cells bearing ostensibly stable reciprocal translocations. Accordingly, it has been definitively demonstrated that the frequency of unstable aberrations, and to a lesser but still significant extent, the frequency of translocations, are not constant but decline with time from the levels initially observed following radiation exposure (Matsumoto et al 1998;Tucker et al 1997Tucker et al , 2004Tucker et al , 2005, though the frequency of reciprocal translocations may plateau above preirradiation levels following the initial decline. The initial decline may be due at least in part to the co-occurrence of reciprocal translocations with unstable forms of chromosome damage (Tucker 2008).…”

Section: Does Error-prone Dna Repair Results In An Increase In Risk?mentioning

confidence: 96%

Checking the Foundation: Recent Radiobiology and the Linear No-Threshold Theory

Ulsh

2010

Health Physics

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The linear no-threshold (LNT) theory has been adopted as the foundation of radiation protection standards and risk estimation for several decades. The "microdosimetric argument" has been offered in support of the LNT theory. This argument postulates that energy is deposited in critical cellular targets by radiation in a linear fashion across all doses down to zero, and that this in turn implies a linear relationship between dose and biological effect across all doses. This paper examines whether the microdosimetric argument holds at the lowest levels of biological organization following low dose, low dose-rate exposures to ionizing radiation. The assumptions of the microdosimetric argument are evaluated in light of recent radiobiological studies on radiation damage in biological molecules and cellular and tissue level responses to radiation damage. There is strong evidence that radiation initially deposits energy in biological molecules (e.g., DNA) in a linear fashion, and that this energy deposition results in various forms of prompt DNA damage that may be produced in a pattern that is distinct from endogenous (e.g., oxidative) damage. However, a large and rapidly growing body of radiobiological evidence indicates that cell and tissue level responses to this damage, particularly at low doses and/or dose-rates, are nonlinear and may exhibit thresholds. To the extent that responses observed at lower levels of biological organization in vitro are predictive of carcinogenesis observed in vivo, this evidence directly contradicts the assumptions upon which the microdosimetric argument is based.

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Section: Does Error-prone Dna Repair Results In An Increase In Risk?mentioning

confidence: 96%

Checking the Foundation: Recent Radiobiology and the Linear No-Threshold Theory

Ulsh

2010

Health Physics

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“…The frequency of stable translocations is thought to increase with age because they are induced throughout life and undergo little negative selection during mitosis [33,39,40]. However, the reason(s) translocation frequencies increase in a curvilinear manner is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Some cells were cultured for as long as 72 hours. Although these cultures will contain many cells in their second division, this should not have a large effect on the translocation yield because translocation frequencies in cultured human peripheral blood lymphocytes do not show major changes during the first 72 hours [33].…”

Section: Blood Sample Collection and Culture Methodsmentioning

confidence: 99%

See 1 more Smart Citation

International study of factors affecting human chromosome translocations

Sigurdson

Hauptmann

et al. 2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

Self Cite

122

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Abstract*Correspondence, page proofs, and reprint requests to: James D. Tucker, Ph.D., Department of Biological Sciences, 1370 Biological Sciences Building, 5047 Gullen Mall, Wayne State University, Detroit, TEL: (313) 577-2783, FAX: (313) 577-3602, EMAIL: E-mail: jtucker@biology.biosci.wayne.edu. z Retired Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from sixteen laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages vs. a linear relationship (p <0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p<0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes. NIH Public Access

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“…In contrast, chromosome translocations are compatible with cell survival. For this reason they show much greater persistence through cell division [21,22] and consequently their frequencies are believed to integrate the effect of chronic exposure [23-25]. Thus, while PCE exposure is of concern to human health, investigations of possible cumulative genetic damage resulting from exposure are lacking.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic analysis of an exposed-referent study: perchloroethylene-exposed dry cleaners compared to unexposed laundry workers

et al. 2011

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BackgroundSignificant numbers of people are exposed to tetrachloroethylene (perchloroethylene, PCE) every year, including workers in the dry cleaning industry. Adverse health effects have been associated with PCE exposure. However, investigations of possible cumulative cytogenetic damage resulting from PCE exposure are lacking.MethodsEighteen dry cleaning workers and 18 laundry workers (unexposed controls) provided a peripheral blood sample for cytogenetic analysis by whole chromosome painting. Pre-shift exhaled air on these same participants was collected and analyzed for PCE levels. The laundry workers were matched to the dry cleaners on race, age, and smoking status. The relationships between levels of cytological damage and exposures (including PCE levels in the shop and in workers' blood, packyears, cumulative alcohol consumption, and age) were compared with correlation coefficients and t-tests. Multiple linear regressions considered blood PCE, packyears, alcohol, and age.ResultsThere were no significant differences between the PCE-exposed dry cleaners and the laundry workers for chromosome translocation frequencies, but PCE levels were significantly correlated with percentage of cells with acentric fragments (R2 = 0.488, p < 0.026).ConclusionsThere does not appear to be a strong effect in these dry cleaning workers of PCE exposure on persistent chromosome damage as measured by translocations. However, the correlation between frequencies of acentric fragments and PCE exposure level suggests that recent exposures to PCE may induce transient genetic damage. More heavily exposed participants and a larger sample size will be needed to determine whether PCE exposure induces significant levels of persistent chromosome damage.

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Persistence of chromosome aberrations following acute radiation: I, PAINT translocations, dicentrics, rings, fragments, and insertions

Cited by 32 publications

References 31 publications

Checking the Foundation: Recent Radiobiology and the Linear No-Threshold Theory

Checking the Foundation: Recent Radiobiology and the Linear No-Threshold Theory

International study of factors affecting human chromosome translocations

Cytogenetic analysis of an exposed-referent study: perchloroethylene-exposed dry cleaners compared to unexposed laundry workers

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