Persistence of circulating t(14;18)-positive cells in long-term remission after radiation therapy for localized-stage follicular lymphoma.

Finke, Jürgen; Slanina, J; Lange, W.; Dölken, Gottfried

doi:10.1200/jco.1993.11.9.1668

Cited by 76 publications

(20 citation statements)

References 24 publications

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“…In our trial, we confirmed the prognostic value of PCR negativity post transplantation and also the possibility of very long complete remission in some patients who remain PCR-positive, as previously described in stage I-II localized FL treated by radiotherapy alone. 34 In conclusion, this phase II trial appears encouraging and large phase III trials are therefore needed to show the superiority of purged or unpurged autologous stem cell transplantation in comparison with conventional chemotherapy in the treatment of advanced stage of FL with poor prognosis.…”

Section: Bone Marrow Transplantationmentioning

confidence: 99%

Value of autologous stem cell transplantation with purged bone marrow as first-line therapy for follicular lymphoma with high tumor burden: a GOELAMS phase II study

Colombat

Cornillet

Deconinck

et al. 2000

Bone Marrow Transplant

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Summary:This prospective phase II study was undertaken to evaluate the efficacy and toxicity of early intensive therapy followed by purged autologous bone marrow transplantation (ABMT) in patients with follicular lymphoma with high tumor burden. All patients received the VCAP regimen (vindesine, cyclophosphamide, doxorubicin and prednisone) as conventional chemotherapy and DHAP as second-line therapy. Twenty-nine consecutive patients were included in the study. Twentyseven patients were grafted, seven in first complete remission (CR) and 20 in first partial remission (PR). Preparative therapy consisted of cyclophosphamide and total body irradiation (TBI) in all the patients. With a median follow-up of 6 years, the actuarial overall survival is 64% and the actuarial event-free survival is 55%. Two treatment-related early deaths were observed. Eleven patients were informative for serial PCR analysis of minimal residual disease after ABMT: two relapsed, four remained disease-free with PCR positivity and five were disease-free with PCR negativity. These encouraging results lay the basis of future prospective randomized trials comparing autologous stem cell transplantation as front-line treatment with conventional chemotherapy for patients with bad prognostic factors. Bone Marrow Transplantation (2000) 26, 971-977.

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Section: Bone Marrow Transplantationmentioning

confidence: 99%

Value of autologous stem cell transplantation with purged bone marrow as first-line therapy for follicular lymphoma with high tumor burden: a GOELAMS phase II study

Colombat

Cornillet

Deconinck

et al. 2000

Bone Marrow Transplant

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“…16,17 Since the breakpoints cluster within the BCL-2 gene on chromosome 18 within small regions [18][19][20] and on chromosome 14 within the J-segments of the IgH locus, 21 a PCR technique has been established for the amplification of t(14;18) translocation fragments with a sensitivity up to one positive cell in 10 5-6 normal cells. [22][23][24] Therefore, this technique provides an excellent opportunity to monitor residual disease in specific situations, ie CR after radiotherapy, chemotherapy and after high-dose chemotherapy supported by bone marrow or peripheral stem cell transplantation, including bone marrow purging. 25 Molecular studies aimed at monitoring FL patients in CR after successful ABMT are hampered by some intriguing findings: (1) 29,30 the multiple tube approach 31 or by increasing the DNA amount in a single PCR up to 5-10 g. 30 All these studies have shown, that many healthy individuals carry more than one t(14;18)-positive cell clone.…”

mentioning

confidence: 99%

Quantitative detection of t(14;18)-positive cells in patients with follicular lymphoma before and after autologous bone marrow transplantation

Hirt

Dölken

2000

Bone Marrow Transplant

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Summary:The aim of this study was to evaluate whether a quantitative analysis of circulating t(14;18)-positive cells is of prognostic significance in patients with follicular lymphoma (FL) after myelo-ablative therapy supported by ABMT. We tested DNA from primary lymphoma tissue as well as PBMC before and after ABMT from 15 patients for the presence of the t(14;18) translocation. Nine patients showed a t(14;18) translocation, six patients were t(14;18)-negative.

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“…Price et al (1991) and Finke et al (1993) report the presence of t(14; 18)-positive circulating cells in patients in long-term complete remission after conventional chemotherapy. Lambrechts et al (1994) show no correlation between the presence or absence of peripheral blood positive cells and clinical outcome.…”

Section: Resultsmentioning

confidence: 99%

Clinical relevance of bcl-2(MBR)/JH rearrangement detected by polymerase chain reaction in the peripheral blood of patients with follicular lymphoma

Bertoni

Bosshard²,

Ricevuto³

et al. 1997

Br J Cancer

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Summary We evaluated the prognostic role of peripheral blood polymerase chain reaction (PCR) assay for detection of the bcl-2(MBR)/JH rearrangement in 59 patients with follicular lymphoma (FL) treated at our centre since 1989. Thirty-five (59%) patients were bcl-2/JH positive and 24 (41%) were negative in the peripheral blood at diagnosis. Peripheral blood bcl-2/JH rearrangement detection at diagnosis had no relation to overall survival (OS) and time to progression (TTP). Peripheral blood PCR assay was performed post treatment in 17 patients who were bcl-2/JH positive at diagnosis. Fourteen of the patients (82%, 95% Cl 56-96%) became bcl-2/JH negative. Nine of these patients were further analysed during follow-up and, after several months, circulating cells carrying the bcl-2/JH rearrangement reappeared in five of the nine patients. Peripheral blood clearance of bcl-2/JH-positive cells was correlated with better overall survival (log-rank P < 0.05) but not with TTP.Our data confirmed that bcl-2(MBR)/JH rearrangement detection by PCR at diagnosis is not a prognostic factor in follicular lymphoma. In our series, clearance of circulating bcl-2/JH-positive cells appeared to correlate with better overall survival. Post-treatment examination of the peripheral blood by PCR may have clinical relevance for prediction of the survival pattern of the patients.

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Persistence of circulating t(14;18)-positive cells in long-term remission after radiation therapy for localized-stage follicular lymphoma.

Cited by 76 publications

References 24 publications

Value of autologous stem cell transplantation with purged bone marrow as first-line therapy for follicular lymphoma with high tumor burden: a GOELAMS phase II study

Value of autologous stem cell transplantation with purged bone marrow as first-line therapy for follicular lymphoma with high tumor burden: a GOELAMS phase II study

Quantitative detection of t(14;18)-positive cells in patients with follicular lymphoma before and after autologous bone marrow transplantation

Clinical relevance of bcl-2(MBR)/JH rearrangement detected by polymerase chain reaction in the peripheral blood of patients with follicular lymphoma

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