2003
DOI: 10.1159/000070705
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Persistence of the Nitric Oxide Pathway in the Aorta of Hypercholesterolemic Apolipoprotein-E-Deficient Mice

Abstract: The markers of the bioavailability of NO (endothelium-dependent relaxation to acetylcholine and cyclic GMP content) in the thoracic aorta of apolipoprotein-E-deficient (ApoE KO) mice, 20 weeks old with enriched cholesterol diet or 35 weeks old on regular chow, are not decreased, in contrast with other models of atherosclerosis. However, severe hypercholesterolemia, the presence of atherosclerotic lesions and increased NADPH oxidase activity have been reported as early as at 20 weeks of age. The present experim… Show more

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Cited by 19 publications
(20 citation statements)
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“…4,5 Although endothelial dysfunction has been considered one of the early steps in atherosclerosis, 1 a normal endothelial function of the aorta was reported recently in apoE mice at 35 weeks of age. 9 In the present study, we evaluated the endothelial function of resistance vessels in young (10 to 12 weeks old) apoE mice and also observed a normal endothelial function. The main candidates that could explain the normal endothelial function in apoE fed with a normal chow diet would be the overexpression of NO synthase or an increased production of superoxide dismutase and catalase enzymes.…”
Section: Discussionmentioning
confidence: 86%
See 1 more Smart Citation
“…4,5 Although endothelial dysfunction has been considered one of the early steps in atherosclerosis, 1 a normal endothelial function of the aorta was reported recently in apoE mice at 35 weeks of age. 9 In the present study, we evaluated the endothelial function of resistance vessels in young (10 to 12 weeks old) apoE mice and also observed a normal endothelial function. The main candidates that could explain the normal endothelial function in apoE fed with a normal chow diet would be the overexpression of NO synthase or an increased production of superoxide dismutase and catalase enzymes.…”
Section: Discussionmentioning
confidence: 86%
“…4,5 In this experimental model, an endothelial dysfunction has been reported under a Western-type cholesterol-rich diet 6 -8 but not under a normal chow diet. 9 Arterial hypertension has also been associated with changes in endothelial function and in vascular smooth muscle cell reactivity to contractile agents. 10 In addition, arterial hypertension induced by endogenous or administered angiotensin II aggravates the atherosclerotic process in apoE mice.…”
mentioning
confidence: 99%
“…The investigator was blinded to genotype or treatment for all experiments, and endothelial vasorelaxation was studied as described previously (45)(46)(47)(48). Thoracic aortas from the indicated mice were carefully excised and quickly immersed in Krebs Henseleit solution (118 mM NaCl, 5.4 mM KCl, 1.2 mM MgCl2, 2.5 mM CaCl2, 22 mM NaHCO3, 1.2 mM NaH2PO4, and 10.1 mM glucose) aerated with 95% O2 and 5% CO2 (37°C, pH 7.4).…”
Section: Methodsmentioning
confidence: 99%
“…9,60,64 -66 Vascular expression of CuZn-SOD and Mn-SOD mRNA change in a temporal pattern during atherosclerosis (initially increase, then decrease over time). 67,68 There is a marked increase in Mn-SOD expression in cerebral arteries in bacterial meningitis, 69 and Mn-SOD expression is increased in the vessel wall in models of chronic hypertension. 53,54,70 In nonvascular tissue, Ang II increases levels of Mn-SOD protein but also decreases activity of MnSOD caused by peroxynitrite-induced tyrosine protein nitration.…”
mentioning
confidence: 99%