Persistent B19 infection in immunocompetent individuals: implications for transfusion safety

Lefrère, Jean‐Jacques

doi:10.1182/blood-2005-03-1053

Cited by 131 publications

(108 citation statements)

References 34 publications

(37 reference statements)

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“…In light of the infrequency of human parvovirus reinfections in general, virus types 2 and 3 have been encountered in blood of immunodeficient individuals conspicuously often (13,14,16,17), suggesting that they might have been released from tissue persistence. In accordance with this view, the maintenance of the Bioportfolio could involve a dynamic interplay between viral replication and the immune surveillance of the host, as has been discussed for the occasional erythrovirus persistence in blood (38,10). We nevertheless believe that an immunological difference does not explain the restrictive occurrence of type 2 viral DNA in tissues of the elderly because of the similarity among the three virus types (15,43) and the paucity of viremic (15,16) type 2 infections during recent years, as shown here.…”

Section: Resultsmentioning

confidence: 82%

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Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Norja

Hokynar

Aaltonen³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

253

216

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Human erythrovirus is a minute, single-stranded DNA virus causing many diseases, including erythema infectiosum, arthropathy, and fetal death. After primary infection, the viral genomes persist in solid tissues. Besides the prototype, virus type 1, two major variants (virus types 2 and 3) have been identified recently, the clinical significance and epidemiology of which are mostly unknown. We examined 523 samples of skin, synovium, tonsil, or liver (birth year range, 1913-2000), and 1,640 sera, by qualitative and quantitative molecular assays for the DNA of human erythroviruses. Virus types 1 and 2 were found in 132 (25%) and 58 (11%) tissues, respectively. DNA of virus type 1 was found in all age groups, whereas that of type 2 was strictly confined to those subjects born before 1973 (P < 0.001). Correspondingly, the sera from the past two decades contained DNA of type 1 but not type 2 or 3. Our data suggest strongly that the newly identified human erythrovirus type 2 as well as the prototype 1 circulated in Northern and Central Europe in equal frequency, more than half a century ago, whereafter type 2 disappeared from circulation. Type 3 never attained wide occurrence in this area during the past >70 years. The erythrovirus DNA persistence in human tissues is lifelong and represents a source of information about our past, the Bioportfolio, which, at the individual level, provides a registry of one's infectious encounters, and at the population level, a database for epidemiological and phylogenetic analyses.epidemiology ͉ gene therapy ͉ parvovirus ͉ phylogeny ͉ single-stranded DNA

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Section: Resultsmentioning

confidence: 82%

“…The virus multiplies restrictively in the erythroid precursor cells of the bone marrow (5), giving rise to high-titer viremia, which, according to sensitive techniques, subsides more slowly than thought previously (6)(7)(8)(9)(10). The viral DNA genome was long considered highly stable and the species, phylogenically monolithic (11).…”

mentioning

confidence: 99%

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Norja

Hokynar

Aaltonen³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

253

216

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“…A peculiar characteristic of B19V infection is that not always the virus is cleared and in many cases it can establish persistency in different body tissues [4]. Persistent B19V infection has been observed in immunologically normal individuals [5] including qualified blood donors [6]. The presence of persistent B19V infection in blood donors raises serious questions regarding transfusion safety of the obtained hemoderivatives, especially plasma products and packed erythrocytes.…”

mentioning

confidence: 99%

Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

Slavov

Otaguiri

Covas

et al. 2015

Indian J Hematol Blood Transfus

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The infection of human parvovirus B19 (B19V) is a common event in the general population, including volunteer blood donors. In some cases it can be asymptomatic and can remain persistent for a long period of time. The objective of this study was to examine the B19V DNA prevalence and viral load in first-time volunteer blood donors. Blood samples were collected from 91 primary blood donors at the Regional Blood Center of Ribeirão Preto, Southeast Brazil. Viral detection and quantitation was performed by an in-house TaqMan Ò real-time PCR with high sensitivity. B19V DNA was detected in one male blood donor (1.0 %) and was characterized by a very low viral load (537.36 copies/mL). Our studies demonstrate that B19V DNA at low titer may be present in apparently healthy individuals. Sensitive molecular diagnostic tools can be applied for the screening of fresh blood derived products in order to prevent transfusion-transmitted B19V infection. Keywords Human parvovirus B19 Á B19V Á Viral quantitation Á Blood donorsHuman parvovirus B19 (B19V), a small icosahedral virus (19-22 nm), is the prototype member of the Erythrovirus genus (Parvoviridae family). B19V infection is a common finding in the general population and demonstrates marked seasonality. Clinical manifestations of B19V infection in children and adults may include erythema infectiosum (fifth disease) or arthropathy, nevertheless, great proportion of the infected individuals remain asymptomatic [1]. Therefore, in the general population B19V infections among blood donors can be considered a normal event and many of them have asymptomatic course [2,3]. A peculiar characteristic of B19V infection is that not always the virus is cleared and in many cases it can establish persistency in different body tissues [4]. Persistent B19V infection has been observed in immunologically normal individuals [5] including qualified blood donors [6]. The presence of persistent B19V infection in blood donors raises serious questions regarding transfusion safety of the obtained hemoderivatives, especially plasma products and packed erythrocytes.Moreover, B19V is highly resilient to almost all virus inactivation procedures [7], causes infections with a high viral load and these properties predispose to easy viral contamination of the blood products. The relevance of the low B19V load in asymptomatic donors and for the respective recipients of blood is still unclear. Therefore, the objective of this study was to evaluate B19V DNA prevalence and viral load (VL) during 1 month blood collection in a non-metropolitan blood transfusion center in Southeast Brazil (Ribeirão Preto city, Western part of the São Paulo State).Ninety one peripheral blood samples were collected from 91 first time volunteer blood donors (mean age 35.7 years, 18-65 years, 55 % males). All of them were approved on the preliminary interview for blood donation & Svetoslav Nanev Slavov

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“…Se ha postulado que B19 podría integrarse al genoma celular del hospedero. Argumentos que apoyan esta idea son aquellos basados en la similitud de este agente con el virus adeno-asociado (AAV), el cual se integra de manera sitio-específi ca al cromosoma 19 en presencia de adenovirus o herpesvirus 28 . Otras evidencias establecen que B19 podría mantenerse en forma episomal por largos períodos de tiempo, produciéndose partículas virales sólo ocasionalmente, lo que llevaría a una estimulación constante del sistema inmune 29 .…”

Section: Discussionunclassified

Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile

et al. 2011

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Persistent B19 infection in immunocompetent individuals: implications for transfusion safety

Cited by 131 publications

References 34 publications

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Prevalence and Viral Load of Human Parvovirus B19 (B19V) Among Blood Donors in South-East Brazil

Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile

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