We have observed the effect of the Aharonov-Casher (AC) interference on the spectrum of a superconducting system containing a symmetric Cooper pair box (CPB) and a large inductance. By varying the charge ng induced on the CPB island, we observed oscillations of the device spectrum with the period ∆ng = 2e. These oscillations are attributed to the charge-controlled AC interference between the fluxon tunneling processes in the CPB Josephson junctions. The measured phase and charge dependences of the frequencies of the |0 → |1 and |0 → |2 transitions are in good agreement with our numerical simulations. Almost complete suppression of the tunneling due to destructive interference has been observed for the charge ng = e(2n + 1). The CPB in this regime enables fluxon pairing, which can be used for the development of parity-protected superconducting qubits.The Aharonov-Casher (AC) effect is a non-local topological effect: the wave function of a neutral particle with magnetic moment moving in two dimensions around a charge acquires a phase shift proportional to the charge [1]. This effect has been observed in experiments with neutrons, atoms, and solid-state semiconductor systems (see, e.g., [2][3][4] and references therein). Similar effects have been predicted for superconducting networks of nanoscale superconducting islands coupled by Josephson junctions. For example, the wave function of the flux vortices (fluxons) moving in such a network should acquire a phase that depends on the charge on superconducting islands [5]. Indeed, oscillations of the network resistance in the flux-flow regime have been observed as a function of the gate-induced island charge [6]; these oscillations have been attributed to the interference associated with the AC phase. However, this attribution is not unambiguous, because qualitatively similar phenomena can be produced by the Coulomb-blockade effect due to the quantization of charge on the superconducting islands [7].More recently, indirect evidence for the AC effect in superconducting circuits has been obtained in the study of suppression of the macroscopic phase coherence in onedimensional (1D) chains of Josephson junctions by quantum fluctuations [8]. The quantum phase slips (QPS) in the junctions can be viewed as the charge-sensitive fluxon tunneling [9,10] provided the conditions discussed below are satisfied. Microwave experiments [11] have demonstrated that dephasing of a fluxonium, a small Josephson junction shunted by a 1D Josephson chain, can be due to the effect of fluctuating charges on the QPS in the chain. Applications of the AC effect in classical Josephson devices have been discussed in Refs. [7,12].In this Letter we describe microwave experiments which provide direct evidence for the charge-dependent interference between the amplitudes of fluxon tunneling. We have studied the microwave resonances of the device consisting of two nominally identical Josephson junctions separated by a nanoscale superconducting island (the socalled Cooper-pair box, CPB) and a large inductanc...