Persistent Luminescence Nanophosphor Involved Near-Infrared Optical Bioimaging for Investigation of Foodborne Probiotics Biodistribution in Vivo: A Proof-of-Concept Study

Abstract

Probiotics has attracted great attention in food nutrition and safety research field, but thus far there are limited analytical techniques for visualized and real-time monitoring of the probiotics when they are ingested in vivo. Herein, the optical bioimaging technique has been introduced for investigation of foodborne probiotics biodistribution in vivo, employing the near-infrared (NIR) emitting persistent luminescence nanophosphors (PLNPs) of Cr-doped zinc gallogermanate (ZGGO) as the contrast nanoprobes. The ultrabrightness, super long afterglow, polydispersed size, low toxicity, and excellent photostability and biocompatibility of PLNPs were demonstrated to be qualified as a tracer for labeling probiotics via antibody (anti-Gram positive bacteria LTA antibody) recognition as well as contrast agent for long-term bioimaging the probiotics. In vivo optical bioimaging assay showed that the LTA antibody functionalized ZGGO nanoprobes that could be efficiently tagged to the probiobics were successfully applied for real-time monitoring and nondamaged probing of the biodistribution of probiotics inside the living body after oral administration. This work presents a proof-of-concept that exploited the bioimaging methodology for real-time and nondamaged researching the foodborne probiotics behaviors in vivo, which would open up a novel way of food safety detection and nutrition investigation.

Recent Advances of Persistent Luminescence Nanoparticles in Bioapplications

“…The PLNPs-based nanoplatform was employed to detect other important parameters and biomolecules, such as dopamine, temperature, avidin and foodborne probiotics [83][84][85][86]. Furthermore, PLNPs-based detection can be produced as the assay reporters in the lateral flow assays [87].…”

Tracer technology of probiotics based on optical imaging

“…Liu等人 [75] Figure 4 Scheme illustrating bacterial detection using zwitterionic fluorescent polymer BOD/BE-PSM under different pH conditions [60] 图 5 ZGGO纳米材料抗体探针在体内生物成像示意图 [75] Figure 5 Schematic illustration of the utilization of the antibody ZGGO nanoprobes for orally administrated bioimaging [75] 测和食品安全检测的思路.…”

Competitive Cr3+ occupation in persistent phosphors toward tunable traps distribution for dynamic anti‐counterfeiting

“…Breakthroughs have been made in biological imaging. [5][6][7][8][9][10][11][12][13] So far, there have been few reports about the application of LPPs in dynamic anti-counterfeiting. Therefore, a dynamic anti-counterfeiting model based on the LPL mechanism is established in this paper.…”

“…due to its advantages of no in situ excitation, high signal‐to‐noise ratio, and deep tissue penetration. Breakthroughs have been made in biological imaging 5–13 . So far, there have been few reports about the application of LPPs in dynamic anti‐counterfeiting.…”