Persistent Physical Exercise Rises the Plasma Concentration of Vasohibin-1 in Patients with Peripheral Vascular Disease

High preoperative plasma vasohibin‐1 concentration predicts better prognosis in patients with non–small cell lung carcinoma

“…Consequently, it is of critical importance to determine how to manipulate the plasma concentration of VASH1. We have recently demonstrated that persistent physical exercise raises plasma VASH1 concentrations in patients with peripheral vascular disease . Preoperative physical exercise has been reported to reduce the mortality of patients with NSCLC .…”

“…We have recently shown that this degradation is enhanced in the presence of cancer cells, which indicates that the VASH1 protein secreted by ECs is inactivated in the tumor microenvironment . Here, we applied a VASH1 ELISA assay, which detects antiangiogenic 44‐ and 36‐kDa protein, but not inactive 29‐kDa protein, and evaluated the potential value of preoperative plasma VASH1 concentration as a prognostic factor in patients with resected lung cancer. Our analysis revealed that the high plasma VASH1 group showed a lower relapse and death rate than did the low VASH1 group throughout the observation period.…”

“…Enzyme‐linked immunosorbent assay for VASH1 was performed as described previously . A peptide corresponding to Gly286‐Arg299 (VC) and Ala217‐Lys229 (VR1) of human VASH1 protein (Q7L8A9) were conjugated with keyhole limpet hemocyanin, and these antigens were immunized to A/J mice, and several monoclonal antibodies (mAbs) were prepared .…”

“…The reaction was stopped by addition of stop buffer, and the absorbance at 450 nm was measured using ARVO HTS (PerkinElmer Life Science, Yokohama). This ELISA system detects the 44‐ and 36‐kDa VASH1 form, but not the 29‐kDa VASH1 form …”