<p>Objective of the work was to construct and validate a dispositional tolerance questionnaire that aims to measure the subject's tolerance level in a complex way. Data collection was carried out in an online format. The methodology was approved with 160 students, the average age was 20 years old. In order to validate the presented questionnaire of dispositional tolerance we used methods of internal coherence and reliability of the scale, convergent validity, as well as confirmatory factor analysis to check the compliance of the theoretical factor structure of the questionnaire with the real one. Internal consistency of the methodology was assessed by calculating the value of Cronbach's α coefficients = 0.725. Convergent validity was assessed with the tolerance index questionnaire and the correlation level was 0.601. Factor analysis of the dispositional tolerance questionnaire confirmed the factor structure of the methodology. The meaning of dispositional tolerance was conceptualized and compared with existing concepts of psychological tolerance for further presentation of the psychometric instrument. This questionnaire stands as a method to diagnose tolerance as a subject's attitude. The obtained results confirmed sufficient reliability for using the presented psychometric tool in psychological practice.</p>