Perspectives in the Cell-Based Therapies of Various Aspects of the Spinal Cord Injury-Associated Pathologies: Lessons from the Animal Models

Zawadzka, Małgorzata; Kwaśniewska, Anna; Miazga, Krzysztof; Sławińska, Urszula

doi:10.3390/cells10112995

Cited by 13 publications

(12 citation statements)

References 199 publications

(242 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It not only brings great physical and emotional burdens to the patients and their families but also puts enormous pressure on the clinical management and society as a whole (GBD 2016Neurology Collaborators, 2019Lee et al, 2014;Rubiano et al, 2015). In the past few decades, exciting progress has been made in understanding the pathophysiology of SCI, which has resulted in better treatment paradigms (Cadotte & Fehlings, 2013;David et al, 2019;Fouad et al, 2021;Sofroniew, 2018;Zawadzka et al, 2021). However, the therapeutic effect on clinical patients has not yet achieved satisfactory results and a huge global challenge for the treatment of SCI still exists (Filli & Schwab, 2012;Selvarajah et al, 2015;Hejrati & Fehlings, 2021;Squair et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress disrupts the cytoskeleton of spinal motor neurons

Chen

Wang

et al. 2022

Brain and Behavior

View full text Add to dashboard Cite

Background and aim: Traumatic spinal cord injury (SCI) is a common and devastating central nervous disease, the treatment of which faces many challenges to the medical community and society as a whole. Treatment measures based on oxidative stress of spinal motor neurons during SCI are expected to help restore biological functions of neurons under injury conditions. However, to date, there are no systematic reports regarding oxidative stress on spinal motor neuron injury. Our aim is to better understand and explain the influences and mechanisms of oxidative stress on spinal motor neurons during SCI. Methods:We first exposed VSC4.1 motor neurons to hydrogen peroxide (H 2 O 2 ) and evaluated the effects on cell viability, morphology, cycling, and apoptosis, with an emphasis on the changes to the cytoskeleton and the effect of N-acetyl-Lcysteine (NAC) on these changes. Then, we investigated the effects of NAC on these cytoskeletal changes in vitro and in vivo. Results:We found that H 2 O 2 caused severe damage to the normal cytoskeleton, leading to a reduction in neurite length and number, rearrangement of the actin cytoskeleton, and disorder of the microtubules and neurofilaments in VSC4.1. Importantly, NAC attenuated the oxidative damage of spinal motor neurons in vitro and in vivo, promoting the recovery of hindlimb motor ability in mice with SCI at the early stage of injury.

show abstract

Section: Introductionmentioning

confidence: 99%

Oxidative stress disrupts the cytoskeleton of spinal motor neurons

Chen

Wang

et al. 2022

Brain and Behavior

View full text Add to dashboard Cite

show abstract

“…The damage caused by spinal cord injury can lead to a cascade of bio-molecular events, which starts with tissue bruising or tearing caused by mechanical damage that leads to the disruption of the nerve fibers and blood vessels, deterioration of nerve and glial cells, ischemia, and tissue edema [ 25 ]. Following the initial impact, the various bio-molecular events, such as edema, inflammation, neuronal and glial necrosis, or apoptosis can spread beyond the initial site of injury, but ultimately generate a necrotic zone surrounded by glial/fibrotic scarring.…”

Section: General Characteristics Of the Tsci Cellular Biologymentioning

confidence: 99%

Main Cations and Cellular Biology of Traumatic Spinal Cord Injury

Munteanu

Rotariu

Turnea

et al. 2022

Cells

View full text Add to dashboard Cite

Traumatic spinal cord injury is a life-changing condition with a significant socio-economic impact on patients, their relatives, their caregivers, and even the community. Despite considerable medical advances, there is still a lack of options for the effective treatment of these patients. The major complexity and significant disabling potential of the pathophysiology that spinal cord trauma triggers are the main factors that have led to incremental scientific research on this topic, including trying to describe the molecular and cellular mechanisms that regulate spinal cord repair and regeneration. Scientists have identified various practical approaches to promote cell growth and survival, remyelination, and neuroplasticity in this part of the central nervous system. This review focuses on specific detailed aspects of the involvement of cations in the cell biology of such pathology and on the possibility of repairing damaged spinal cord tissue. In this context, the cellular biology of sodium, potassium, lithium, calcium, and magnesium is essential for understanding the related pathophysiology and also the possibilities to counteract the harmful effects of traumatic events. Lithium, sodium, potassium—monovalent cations—and calcium and magnesium—bivalent cations—can influence many protein–protein interactions, gene transcription, ion channel functions, cellular energy processes—phosphorylation, oxidation—inflammation, etc. For data systematization and synthesis, we used the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) methodology, trying to make, as far as possible, some order in seeing the “big forest” instead of “trees”. Although we would have expected a large number of articles to address the topic, we were still surprised to find only 51 unique articles after removing duplicates from the 207 articles initially identified. Our article integrates data on many biochemical processes influenced by cations at the molecular level to understand the real possibilities of therapeutic intervention—which must maintain a very narrow balance in cell ion concentrations. Multimolecular, multi-cellular: neuronal cells, glial cells, non-neuronal cells, but also multi-ionic interactions play an important role in the balance between neuro-degenerative pathophysiological processes and the development of effective neuroprotective strategies. This article emphasizes the need for studying cation dynamics as an important future direction.

show abstract

“…These roles include modulating inflammatory response, providing nutritional support, regenerating lost neural circuitries, and remyelinating denuded axons, which can improve symptoms (Zawadzka et al, 2021). Cells such as neural stem cells (NSCs), mesenchymal stem cells (MSCs), Schwann cells (SCs), and OECs have been shown…”

Section: Introductionmentioning

confidence: 99%

Spinal cord injury: Olfactory ensheathing cell‐based therapeutic strategies

Chen,

Liu,

Stavrinou

et al. 2023

J of Neuroscience Research

View full text Add to dashboard Cite

Spinal cord injury (SCI) is a highly disabling neurological disorder that is difficult to treat due to its complex pathophysiology and nerve regeneration difficulties. Hence, effective SCI treatments are necessary. Olfactory ensheathing cells (OECs), glial cells derived from the olfactory bulb or mucosa, are ideal candidates for SCI treatment because of their neuroprotective and regenerative properties, ample supply, and convenience. In vitro, animal model, and human trial studies have reported discoveries on OEC transplantation; however, shortcomings have also been demonstrated. Recent studies have optimized various OEC transplantation strategies, including drug integration, biomaterials, and gene editing. This review aims to introduce OECs mechanisms in repairing SCI, summarize the research progress of OEC transplantation‐optimized strategies, and provide novel research ideas for SCI treatment.

show abstract

Perspectives in the Cell-Based Therapies of Various Aspects of the Spinal Cord Injury-Associated Pathologies: Lessons from the Animal Models

Cited by 13 publications

References 199 publications

Oxidative stress disrupts the cytoskeleton of spinal motor neurons

Oxidative stress disrupts the cytoskeleton of spinal motor neurons

Main Cations and Cellular Biology of Traumatic Spinal Cord Injury

Spinal cord injury: Olfactory ensheathing cell‐based therapeutic strategies

Contact Info

Product

Resources

About