Perspectives on scaling production of adipose tissue for food applications

Yuen, John S.K.; Stout, Andrew J.; Kawecki, N. Stephanie; Letcher, Sophia M.; Theodossiou, Sophia K.; Cohen, Julian M.; Barrick, Brigid M.; Saad, Michael K.; Rubio, Natalie R.; Pietropinto, Jaymie A.; DiCindio, Hailey; Zhang, Sabrina W; Rowat, Amy C.; Kaplan, David L.

doi:10.1016/j.biomaterials.2021.121273

Cited by 22 publications

(23 citation statements)

References 380 publications

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“…DF‐1 is an immortal chicken embryonic cell line and shown to be capable of differentiating into mature adipocytes when the cell was treated with insulin, fatty acids, and all‐trans retinoic acid in medium supplemented with chicken serum (Lee et al., 2021). Induced pluripotent stem (iPS) cells are another potential cell type that may be suitable for scalable production of adipocytes across the wide range of animal species (Stanton et al., 2019; Yuen et al., 2022). iPS cells are generated by introducing reprogramming genes in somatic cells and exhibit superior growth properties.…”

Section: Technological Review On Protocols For Adipocytes From Meat A...mentioning

confidence: 99%

“…In some species, additional names and exclusion terms were included for search; "murine" for mouse, "bovine" or "beef" and NOT "FBS" for cow, "porcine" or "pork" for pig, and NOT "fish oil" for fish. As the number of papers on cows, pigs, chicken, and fish was very small compared to that on humans, mice, and rats, separate graphs are shown in the right side (Yuen et al, 2022). It is also possible to engineer the cells to produce additional nutrients that are not readily enriched in animal meats, as demonstrated by a study where antioxidant carotenoids were produced by engineering bovine muscle cells (Stout et al, 2020).…”

Section: Species Differences In Adipose Tissuementioning

confidence: 99%

“…In addition, alteration of fatty acid profiles of intramuscular fat through the feeding strategy may not be energetically favorable, as fatty acids are also delivered to other lipid‐rich tissues such as liver, brain, and subcutaneous/visceral adipose tissues. On the other hand, cellular agriculture offers an alternative strategy to more directly control the composition of those fatty acids in meat products through altering culture media components of cultivated adipocytes (Yuen et al., 2022). It is also possible to engineer the cells to produce additional nutrients that are not readily enriched in animal meats, as demonstrated by a study where antioxidant carotenoids were produced by engineering bovine muscle cells (Stout et al., 2020).…”

Section: Species Differences In Adipose Tissuementioning

confidence: 99%

“…Various scaffolding design principles are worth considering. For instance, substrate stiffness that is almost similar to the native environment of fat tissue enhances human adipogenesis (Fish et al., 2020; Ng & Kurisawa, 2021; Yuen et al., 2022). Besides its primary function of providing structure to the final product, these scaffolding materials must be edible, degradable or reusable and safe, and furnish taste, texture, and, to a certain extent, digestibility, and nutrition of health benefit.…”

Section: Technical Opportunities and Challengesmentioning

confidence: 99%

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Reassessment of adipocyte technology for cellular agriculture of alternative fat

Sugii

Wong

Lwin

et al. 2022

Comp Rev Food Sci Food Safe

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Alternative proteins, such as cultivated meat, have recently attracted significant attention as novel and sustainable food. Fat tissue/cell is an important component of meat that makes organoleptic and nutritional contributions. Although adipocyte biology is relatively well investigated, there is limited focus on the specific techniques and strategies to produce cultivated fat from agricultural animals. In the assumed standard workflow, stem/progenitor cell lines are derived from tissues of animals, cultured for expansion, and differentiated into mature adipocytes. Here, we compile information from literature related to cell isolation, growth, differentiation, and analysis from bovine, porcine, chicken, other livestock, and seafood species. A diverse range of tissue sources, cell isolation methods, cell types, growth media, differentiation cocktails, and analytical methods for measuring adipogenic levels were used across species. Based on our analysis, we identify opportunities and challenges in advancing new technology era toward producing “alternative fat” that is suitable for human consumption.

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Section: Technological Review On Protocols For Adipocytes From Meat A...mentioning

confidence: 99%

Section: Species Differences In Adipose Tissuementioning

confidence: 99%

Section: Species Differences In Adipose Tissuementioning

confidence: 99%

Section: Technical Opportunities and Challengesmentioning

confidence: 99%

See 2 more Smart Citations

Reassessment of adipocyte technology for cellular agriculture of alternative fat

Sugii

Wong

Lwin

et al. 2022

Comp Rev Food Sci Food Safe

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show abstract

“…So far, the research on cultured meat has mainly focused on cultured muscle tissue [ 9 , 10 ]. However, fat tissue has an important influence on the sensory and texture properties of meat [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Large-Scale Expansion of Porcine Adipose-Derived Stem Cells Based on Microcarriers System for Cultured Meat Production

Song

et al. 2022

Foods

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Cultured meat is an innovative meat-production technology that does not rely on animal husbandry. As a new food component, cultured fat is of great significance to cultured meat. In this study, we isolated adipose-derived stem cells (ADSCs) and identified the purity by immunofluorescence staining of ADSC-specific surface marker proteins CD44 and CD29 and showed that most of the cells were positive for CD29 and CD44. In addition, we detected the expression of FABP4 and Plin1 to confirm that ADSCs differentiated into mature adipocytes at 10 days post-induction. Subsequently, the culture conditions of ADSCs on microcarriers (MCs) were optimized and showed that cell density of living cells reached their highest after 5 days when continuously stirring at 50 rpm. Finally, the expression of FABP4 and PPARγ was detected to confirm the adipogenic differentiation ability of ADSCs on 2D and 3D culture systems and showed that ADSCs maintained their adipogenic differentiation ability after expansion on MCs. In conclusion, this research demonstrated that reliance on MCs to expand ADSCs was a promising approach for production of cultured fat.

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Biofabrication's Contribution to the Evolution of Cultured Meat

Albrecht,

Ahlfeld,

Klatt

et al. 2024

Adv Healthcare Materials

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Cultured Meat (CM) is a growing field in cellular agriculture, driven by the environmental impact of conventional meat production, which contributes to climate change and occupies about 70% of arable land. As demand for meat alternatives rises, research in this area expands. CM production relies on tissue engineering techniques, where a limited number of animal cells are cultured in vitro and processed to create meat‐like tissue comprising muscle and adipose components. Currently, CM is primarily produced on a small scale in pilot facilities. Producing a large cell mass based on suitable cell sources and bioreactors remains challenging. Advanced manufacturing methods and innovative materials are required to subsequently process this cell mass into CM products on a large scale. Consequently, CM is closely linked with biofabrication, a suite of technologies for precisely arranging cellular aggregates and cell‐material composites to construct specific structures, often using robotics. This review provides insights into contemporary biomedical biofabrication technologies, focusing on significant advancements in muscle and adipose tissue biofabrication for CM production. We also discuss novel materials for biofabricating CM, emphasizing their edibility and incorporation of healthful components. Finally, we examine initial studies on biofabricated CM, addressing current limitations and future challenges for large‐scale production.This article is protected by copyright. All rights reserved

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Perspectives on scaling production of adipose tissue for food applications

Cited by 22 publications

References 380 publications

Reassessment of adipocyte technology for cellular agriculture of alternative fat

Reassessment of adipocyte technology for cellular agriculture of alternative fat

Large-Scale Expansion of Porcine Adipose-Derived Stem Cells Based on Microcarriers System for Cultured Meat Production

Biofabrication's Contribution to the Evolution of Cultured Meat

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