Perspectives on the specific targeting of <i>Fusarium graminearum</i> for the development of alternative head blight treatment approaches

Čuperlović-Culf, Miroslava; Loewen, Michèle C.; Rajagopalan, Nandhakishore; Surendra, Anuradha

doi:10.1111/ppa.12726

Cited by 11 publications

(10 citation statements)

References 120 publications

(165 reference statements)

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“…Conventional methods of controlling F. graminearum include application of fungicides and use of more resistant wheat cultivars 71 . More recently, biological control agents 72,73 ,small RNA interference 74,75 and generation of cultivars overexpressing genes conferring F. graminearum resistance [76][77][78] have emerged as potential methods of managing F. graminearum.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, biological control agents 72,73 ,small RNA interference 74,75 and generation of cultivars overexpressing genes conferring F. graminearum resistance [76][77][78] have emerged as potential methods of managing F. graminearum. In any case, existing disease management methods have their limitations 71,79 and increasing fungicide resistance, limited FHB-resistant wheat cultivars and changing climate conditions are confounding factors in keeping FHB under control. Understanding the mechanism of infection by F. graminearum is essential and will potentially result in more specific targets for fungal inhibition to reduce the devastating consequences of this fungal disease.…”

Section: Discussionmentioning

confidence: 99%

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Ste2 receptor-mediated chemotropism of Fusarium graminearum contributes to its pathogenicity against wheat

Sridhar

Trofimova

Subramaniam

et al. 2020

Sci Rep

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Fusarium Head Blight of wheat, caused by the filamentous fungus Fusarium graminearum, leads to devastating global food shortages and economic losses. While many studies have addressed the responses of both wheat and F. graminearum during their interaction, the possibility of fungal chemotropic sensing enabling pathogenicity remains unexplored. Based on recent findings linking the pheromone-sensing G-protein-coupled receptor Ste2 to host-directed chemotropism in Fusarium oxysporum, we investigated the role of the Ste2 receptor and its downstream signaling pathways in mediating chemotropism of F. graminearum. Interestingly, a chemotropic response of growing hyphae towards catalytically active Triticum aestivum 'Roblin' cultivar secreted peroxidases was detected, with deletion of STE2 in F. graminearum leading to loss of the observed response. At the same time, deletion of STE2 significantly decreased infection on germinating wheat coleoptiles, highlighting an association between Ste2, chemotropism and infection by F. graminearum. Further characterization revealed that the peroxidase-directed chemotropism is associated with stimulation of the fungal cell wall integrity mitogen-activated protein kinase signaling cascade. Altogether, this study demonstrates conservation of Ste2-mediated chemotropism by Fusarium species, and its important role in mediating pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ste2 receptor-mediated chemotropism of Fusarium graminearum contributes to its pathogenicity against wheat

Sridhar

Trofimova

Subramaniam

et al. 2020

Sci Rep

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“…Comparisons across five wheat genotypes susceptible to FHB. This file contains seven worksheets: (1) network hub genes; (2) genes involved in the aromatic metabolism; (3) genes involved in the cellular nitrogen metabolism; (4) genes involved in the cinnamic acid biosynthesis; (5) genes involved in the heme activity; (6) genes involved in the oxidoreductase activity; and (7) genes involved in the hydrolase activity.…”

Section: Data Reduction and Partitioningmentioning

confidence: 99%

“…FHB remains a significant pathogenic threat to the agricultural industry; Fg infection decreases grain yields and deposits mycotoxins [ 2 , 3 ]. Wheat breeding programs aimed at addressing FHB have resulted in incremental benefits to date [ 4 ], while chemical control measures remain limited by host-developmental requirements of the pathogen [ 5 ]. Thus, it is imperative that we expand our understanding of these host-pathogen interactions to identify new sources of resistance and antifungal molecular targets.…”

Section: Introductionmentioning

confidence: 99%

Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge

et al. 2021

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Background Treatment of wheat with the phytohormones abscisic acid (ABA) and gibberellic acid (GA) has been shown to affect Fusarium head blight (FHB) disease severity. However, the molecular mechanisms underlying the elicited phenotypes remain unclear. Toward addressing this gap in our knowledge, global transcriptomic profiling was applied to the FHB-susceptible wheat cultivar ‘Fielder’ to map the regulatory responses effected upon treatment with ABA, an ABA receptor antagonist (AS6), or GA in the presence or absence of Fusarium graminearum (Fg) challenge. Results Spike treatments resulted in a total of 30,876 differentially expressed genes (DEGs) identified in ‘Fielder’ (26,004) and the Fg (4872) pathogen. Topology overlap and correlation analyses defined 9689 wheat DEGs as Fg-related across the treatments. Further enrichment analyses demonstrated that these included expression changes within ‘Fielder’ defense responses, cell structural metabolism, molecular transport, and membrane/lipid metabolism. Dysregulation of ABA and GA crosstalk arising from repression of ‘Fielder’ FUS3 was noted. As well, expression of a putative Fg ABA-biosynthetic cytochrome P450 was detected. The co-applied condition of Fg + ABA elicited further up-regulation of phytohormone biosynthesis, as well as SA and ET signaling pathways and cell wall/polyphenolic metabolism. In contrast, co-applied Fg + GA mainly suppressed phytohormone biosynthesis and signaling, while modulating primary and secondary metabolism and flowering. Unexpectedly, co-applied Fg + AS6 did not affect ABA biosynthesis or signaling, but rather elicited antagonistic responses tied to stress, phytohormone transport, and FHB disease-related genes. Conclusions Observed exacerbation (misregulation) of classical defense mechanisms and cell wall fortifications upon ABA treatment are consistent with its ability to promote FHB severity and its proposed role as a fungal effector. In contrast, GA was found to modulate primary and secondary metabolism, suggesting a general metabolic shift underlying its reduction in FHB severity. While AS6 did not antagonize traditional ABA pathways, its impact on host defense and Fg responses imply potential for future investigation. Overall, by comparing these findings to those previously reported for four additional plant genotypes, an additive model of the wheat-Fg interaction is proposed in the context of phytohormone responses.

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“…The most effective approach to preventing trichothecene contamination of cereal grains is to combine disease management strategies with the cultivation of varieties with high levels of FHB resistance. Disease management will not be discussed here, but various reviews are available on the subject [26,120,287,288,289,290,291,292]. Five types or forms of FHB resistance have been described, Type I to Type V (Table 4), which have also been numbered with Arabic numerals or alphabetically and are nicely summarized in Mesterházy [293].…”

Section: Fusarium Resistance To Limit Trichothecene Contamination mentioning

confidence: 99%

Trichothecenes in Cereal Grains – An Update

Foroud

Baines

Gagkaeva

et al. 2019

Toxins

121

105

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Trichothecenes are sesquiterpenoid mycotoxins produced by fungi from the order Hypocreales, including members of the Fusarium genus that infect cereal grain crops. Different trichothecene-producing Fusarium species and strains have different trichothecene chemotypes belonging to the Type A and B class. These fungi cause a disease of small grain cereals, called Fusarium head blight, and their toxins contaminate host tissues. As potent inhibitors of eukaryotic protein synthesis, trichothecenes pose a health risk to human and animal consumers of infected cereal grains. In 2009, Foroud and Eudes published a review of trichothecenes in cereal grains for human consumption. As an update to this review, the work herein provides a comprehensive and multi-disciplinary review of the Fusarium trichothecenes covering topics in chemistry and biochemistry, pathogen biology, trichothecene toxicity, molecular mechanisms of resistance or detoxification, genetics of resistance and breeding strategies to reduce their contamination of wheat and barley.

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Perspectives on the specific targeting of Fusarium graminearum for the development of alternative head blight treatment approaches

Cited by 11 publications

References 120 publications

Ste2 receptor-mediated chemotropism of Fusarium graminearum contributes to its pathogenicity against wheat

Ste2 receptor-mediated chemotropism of Fusarium graminearum contributes to its pathogenicity against wheat

Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge

Trichothecenes in Cereal Grains – An Update

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