Perturbation of an arctic soil microbial community by metal nanoparticles

Kumar, Niraj; Shah, Vishal; Walker, Virginia K.

doi:10.1016/j.jhazmat.2011.04.005

Cited by 160 publications

(86 citation statements)

References 37 publications

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“…Debate remains about how significantly ENPs released into soil will affect soil microbial communities (21,31,35,50). Pure culture studies regularly report both acute toxicity and sublethal effects of ENPs on bacteria through mechanisms including membrane disorganization, DNA damage, surface-coating-related photocatalytic oxidation, and reactive oxygen species (ROS) production (5,10,23,44).…”

Section: Do Enps Affect Soil Bacterial Communities?mentioning

confidence: 99%

“…This suggests that ENPs could be toxins when released into soil, e.g., harming or killing microorganisms. However, while some studies evaluating ENP effects on soil communities reported shifts in bacterial community composition and reduced biomass and enzyme activities (16,21,35), others found subtle (31) or no (50) effects of ENPs on soil microbial communities. The apparent con- tradiction of soil microbial responses to ENPs could be partially caused by the inherent toxicity differences among ENPs (e.g., metal/metal oxides versus fullerenes) (1,26,28) and also possibly by differences among soils used in experiments (49) or experimental conditions.…”

Section: Do Enps Affect Soil Bacterial Communities?mentioning

confidence: 99%

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Identification of Soil Bacteria Susceptible to TiO ₂ and ZnO Nanoparticles

Schimel

Holden

2012

Appl Environ Microbiol

233

124

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ABSTRACTBecause soil is expected to be a major sink for engineered nanoparticles (ENPs) released to the environment, the effects of ENPs on soil processes and the organisms that carry them out should be understood. DNA-based fingerprinting analyses have shown that ENPs alter soil bacterial communities, but specific taxon changes remain unknown. We used bar-coded pyrosequencing to explore the responses of diverse bacterial taxa to two widely used ENPs, nano-TiO2and nano-ZnO, at various doses (0, 0.5, 1.0, and 2.0 mg g−1soil for TiO2; 0.05, 0.1, and 0.5 mg g−1soil for ZnO) in incubated soil microcosms. These ENPs significantly altered the bacterial communities in a dose-dependent manner, with some taxa increasing as a proportion of the community, but more taxa decreasing, indicating that effects mostly reduced diversity. Some of the declining taxa are known to be associated with nitrogen fixation (Rhizobiales,Bradyrhizobiaceae, andBradyrhizobium) and methane oxidation (Methylobacteriaceae), while some positively impacted taxa are known to be associated with the decomposition of recalcitrant organic pollutants (Sphingomonadaceae) and biopolymers including protein (StreptomycetaceaeandStreptomyces), indicating potential consequences to ecosystem-scale processes. The latter was suggested by a positive correlation between protease activity and the relative abundance ofStreptomycetaceae(R= 0.49,P= 0.000) andStreptomyces(R= 0.47,P= 0.000). Our results demonstrate that some metal oxide nanoparticles could affect soil bacterial communities and associated processes through effects on susceptible, narrow-function bacterial taxa.

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Section: Do Enps Affect Soil Bacterial Communities?mentioning

confidence: 99%

Section: Do Enps Affect Soil Bacterial Communities?mentioning

confidence: 99%

Identification of Soil Bacteria Susceptible to TiO ₂ and ZnO Nanoparticles

Schimel

Holden

2012

Appl Environ Microbiol

233

124

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“…Amplification of the partial rRNA gene sequences used forward (GC-338f) and reverse (907r) primers [32,33] as described by Chu et al [34]. After initially inspecting the products (629 bp) on 1% agarose gel, amplified DNAs were separated on 6% (w/v) polyacrylamide (acrylamide:bisacrylamided 37.5:1) gels, using a denaturing gradient of 2.8 to 4.2 M urea and 16 to 24% formamide in 40 mM Tris-acetate, 1 mM EDTA at 65 V and 608C for 20 h. Gels were stained with SYBR Green (Invitrogen), scanned with a ChemiGenius scanner (Syngene), and analyzed in Quantity One image analysis software, version 4.6.7 (Bio-Rad Laboratories), as Kumar et al [8] described. Statistical calculations on band numbers were similar to those described for the FAME analysis.…”

Section: Community Analysesmentioning

confidence: 99%

“…As a result, millions of tonnes of NPs are currently manufactured yearly [6], with every expectation that production will continue to increase. Given these many applications, it is strange that few studies have investigated the influence of NPs on microbial biodiversity in situ [7,8]. This is vital, because it is well known that any change in soil community structure can have significant consequences for ecosystems, including plant growth [9].…”

Section: Introductionmentioning

confidence: 99%

Influence of a nanoparticle mixture on an arctic soil community

Kumar

Shah

Walker

2011

Enviro Toxic and Chemistry

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Interest is growing in understanding not only the impact of individual nanoparticles (NPs) on ecosystems but also the effect of NP mixtures. In the present study, the impact of a combination of three different NPs, silver, copper, and silica (all at 0.022%, w/w), on an arctic microbial community was investigated. After adding the NPs, soil microcosms were incubated for 176 d, and subsequent estimates of microbe diversity were obtained using culture-dependent and culture-independent assessments. The treated soil appeared to show a reduction in the ability to use carbohydrate and amino acid substrates and demonstrated an altered pattern of major fatty acid peaks. Polymerase chain reaction-denaturing gradient gel electrophoresis showed consistent differences in the pattern of predominant rRNA gene sequences. Although this is an initial investigation of soil contaminated with mixed NPs, these results demonstrate that even at the relatively modest concentrations used such pollutants have the potential to disrupt microbial communities.

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“…However, they reported a reduction in cellspecific bacterial activity after a 5-day exposure, which is in contrast to our study, showing enhanced activity rates under in situ conditions. Changes in community structure were also observed for soil bacteria following exposures to TiO2, ZnO, Ag and Cu nanoparticles (Ge et al 2011, Kumar et al 2011.…”

Section: Discussionmentioning

confidence: 90%

Impact of TiO2 nanoparticles on freshwater bacteria from three Swedish lakes

Farkas

Peter

Ciesielski

et al. 2015

Science of The Total Environment

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Due to the rapidly rising production and usage of nano-enabled products, aquatic environments are increasingly exposed to engineered nanoparticles, causing concerns about their potential negative effects. In this study we assessed the effects of uncoated titanium dioxide nanoparticles No UV-induced phototoxic effect of TiO2NP was found in this study. We conclude that in freshwater lakes with high DOC and low chemical element concentrations, uncoated TiO2NPshow an enhanced stability and can significantly reduce bacterial abundance at relatively low exposure concentrations.

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Perturbation of an arctic soil microbial community by metal nanoparticles

Cited by 160 publications

References 37 publications

Identification of Soil Bacteria Susceptible to TiO ₂ and ZnO Nanoparticles

Identification of Soil Bacteria Susceptible to TiO ₂ and ZnO Nanoparticles

Influence of a nanoparticle mixture on an arctic soil community

Impact of TiO2 nanoparticles on freshwater bacteria from three Swedish lakes

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Perturbation of an arctic soil microbial community by metal nanoparticles

Cited by 160 publications

References 37 publications

Identification of Soil Bacteria Susceptible to TiO 2 and ZnO Nanoparticles

Identification of Soil Bacteria Susceptible to TiO 2 and ZnO Nanoparticles

Influence of a nanoparticle mixture on an arctic soil community

Impact of TiO2 nanoparticles on freshwater bacteria from three Swedish lakes

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Product

Resources

About

Identification of Soil Bacteria Susceptible to TiO ₂ and ZnO Nanoparticles

Identification of Soil Bacteria Susceptible to TiO ₂ and ZnO Nanoparticles