Pesticide induced up-regulation of esterase and aldehyde dehydrogenase in indigenous <i>Bacillus</i> spp.

Bhatt, Pankaj; Gangola, Saurabh; Chaudhary, Parul; Khati, Priyanka; Kumar, Govind; Sharma, Anita; Srivastava, Anjana

doi:10.1080/10889868.2019.1569586

Cited by 74 publications

(25 citation statements)

References 34 publications

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“…To calculate k, natural logarithm values of C were plotted against time t. Percent persistence values at different time intervals were calculated in comparison to cypermethrin recovered on the initial day (0) i.e., 1 h after the application (100%). The k of each concentration was computed from the slope of the line [41].…”

Section: Kinetic Analysis Of Cypermethrinmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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Cypermethrin is popularly used as an insecticide in households and agricultural fields, resulting in serious environmental contamination. Rapid and effective techniques that minimize or remove insecticidal residues from the environment are urgently required. However, the currently available cypermethrin-degrading bacterial strains are suboptimal. We aimed to characterize the kinetics and metabolic pathway of highly efficient cypermethrin-degrading Bacillus thuringiensis strain SG4. Strain SG4 effectively degraded cypermethrin under different conditions. The maximum degradation was observed at 32 °C, pH 7.0, and a shaking speed of 110 rpm, and about 80% of the initial dose of cypermethrin (50 mg·L−1) was degraded in minimal salt medium within 15 days. SG4 cells immobilized with sodium alginate provided a higher degradation rate (85.0%) and lower half-life (t1/2) of 5.3 days compared to the 52.9 days of the control. Bioaugmentation of cypermethrin-contaminated soil slurry with strain SG4 significantly enhanced its biodegradation (83.3%). Analysis of the degradation products led to identification of nine metabolites of cypermethrin, which revealed that cypermethrin could be degraded first by cleavage of its ester bond, followed by degradation of the benzene ring, and subsequent metabolism. A new degradation pathway for cypermethrin was proposed based on analysis of the metabolites. We investigated the active role of B. thuringiensis strain SG4 in cypermethrin degradation under various conditions that could be applied in large-scale pollutant treatment.

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Section: Kinetic Analysis Of Cypermethrinmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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“…Microbial system is considered suitable for the biodegradation of synthetic pyrethroids (Bhatt et al, 2019a). Most of the previous work about pyrethroids is based on bacterial degradation.…”

Section: Introductionmentioning

confidence: 99%

Insight Into Microbial Applications for the Biodegradation of Pyrethroid Insecticides

et al. 2019

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Pyrethroids are broad-spectrum insecticides and presence of chiral carbon differentiates among various forms of pyrethroids. Microbial approaches have emerged as a popular solution to counter pyrethroid toxicity to marine life and mammals. Bacterial and fungal strains can effectively degrade pyrethroids into non-toxic compounds. Different strains of bacteria and fungi such as Bacillus spp., Raoultella ornithinolytica, Psudomonas flourescens , Brevibacterium sp., Acinetobactor sp., Aspergillus sp., Candida sp., Trichoderma sp., and Candia spp., are used for the biodegradation of pyrethroids. Hydrolysis of ester bond by enzyme esterase/carboxyl esterase is the initial step in pyrethroid biodegradation. Esterase is found in bacteria, fungi, insect and mammalian liver microsome cells that indicates its hydrolysis ability in living cells. Biodegradation pattern and detected metabolites reveal microbial consumption of pyrethroids as carbon and nitrogen source. In this review, we aim to explore pyrethroid degrading strains, enzymes and metabolites produced by microbial strains. This review paper covers in-depth knowledge of pyrethroids and recommends possible solutions to minimize their environmental toxicity.

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“…Isolated bacterial strain Bacillus cereus 2D showed higher range of pesticide degradation potential (94-86%) within shorter period of time (15 days). Pankaj et al and Bhatt et al [46,44] have reported biodegradation of cypermethrin using Bacillus spp. and found 85% degradation after 15 days under similar conditions.…”

Section: Discussionmentioning

confidence: 99%

“…These unique proteins were further classified into different groups based on their functionality, viz., translational proteins, stress proteins and catabolic enzymes etc. Up-regulation of proteins responsible for substrate transport and energy metabolism during pesticide degradation was reported in different microorganisms [7,46,63].…”

Section: Discussionmentioning

confidence: 99%

Differential analysis of pesticides biodegradation in soil using conventional and high-throughput technology

Gangola

Joshi²,

Kumar

et al. 2021

Preprint

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A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 ppm) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in minimal salt medium with 94, 91, 89 and 86% respectively as revealed by HPLC and GC analysis after 15 days of incubation. Presence of cyclobutane, pyrrolidine, chloroacetic acid, formic acid and decyl ester as major intermediate metabolites of cypermethrin biodegradation was observed in GC-MS analysis. Results based on 16S rDNA sequencing, and phylogenetic analysis showed maximum similarity of 2D with Bacillus cereus (MH341691). Stress responsive and catabolic/ pesticide degrading proteins were over expressed in the presence of cypermethrin in bacteria. Enzyme kinetics of laccase was deduced in the test isolate under normal and pesticide stress conditions. Amplification of laccase gene showed a major band of 1200bp. Maximum copy number of 16S rDNA was seenin uncontaminated soil as compared to pesticide contaminated soil using qRT-PCR. The metagenome sequencing revealed reduction in the population of proteobacteria in contaminated soil as compared to uncontaminated soil but showed dominance of actinobacteria, firmicutes and bacteriodates in pesticide spiked soil. Presence of some new phyla like chloroflexi, planctomycetes, verrucomicrobia was observed followed by extinction of acidobacteria and crenarchaeota in spiked soil. The present study highlights on the potential of 2D bacterial strain i.e., high tolerance level of pesticide, effective biodegradation rate, and presence of laccase gene in bacterial strain 2D, could become a potential biological agent for large-scale treatment of mixture of pesticide (cypermethrin, fipronil, imidacloprid and sulfosulfuron) in natural environment (soil and water).

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Pesticide induced up-regulation of esterase and aldehyde dehydrogenase in indigenous Bacillus spp.

Cited by 74 publications

References 34 publications

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Insight Into Microbial Applications for the Biodegradation of Pyrethroid Insecticides

Differential analysis of pesticides biodegradation in soil using conventional and high-throughput technology

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