With 1.7 million children in the United States with an incarcerated parent, the need to provide evidence-based programming, which helps incarcerated mothers re-establish healthy relationships with their children, is essential. This study examines Parenting, Prison, and Pups, a jail-based parenting course for incarcerated women, integrated with the use of animal-assisted therapy (AAT). Utilizing a mixed-method quasi-experimental design, the authors examined differences between mothers who completed a parenting course with AAT, compared with those who completed the same course without AAT; statistically significant lower rates of parental stress and higher rates of self-esteem and parental knowledge among the AAT group were found. Based on qualitative data, the presence of therapy dogs appeared to encourage communication, trust, and connectedness between group members. These results indicate the importance of using innovative tools to help incarcerated women, who often have long histories of trauma and abuse, to develop healthy bonds with their children.