Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants which have caused worldwide concerns as toxic pollutant. This study reports the concentrations of 15 PAHs in 5 species of fish samples collected along the harbour line, Mumbai, between 2006 and 2008. Among 5 species of fish investigated, Mandeli, Coilia dussimieri, detected the maximum concentration of PAHs (P < 0.05) followed by Doma, Otolithes ruber. The concentration of total and carcinogenic PAHs ranged from 17.43 to 70.44 ng/g wet wt. and 9.49 to 31.23 ng/g wet wt, respectively, among the species tested. The lower-molecular-weight PAHs were detected at highest levels. Estimated intakes of PAHs by fish consumption for the general population were ranged between 1.77 and 10.70 ng/kg body weight/day. Mandeli contributed to the highest intakes of PAHs. The toxic equivalents (TEQs) of PAHs were calculated using a TEQ proposed in literature, and the intake ranged from 8.39 to 15.78 pg TEQ/kg body weight/d. The estimated excess cancer risk value (2.37 × 10 −7 −1.43 × 10 −6 ) from fish consumption for the general population exceeded the guideline value (1.0 × 10 −6 ) for potential cancer risk.