pfeRNAs—A Novel Class of Small Non-coding RNAs With Real Translational Potential

Mei, Yuping; Khan, Hira; Shishikura, Maria; Ishiyama, Shun; Khan, Adil Aziz; Orita, Hajime; Brock, Malcolm V.

doi:10.1016/j.jss.2022.12.002

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…Non-coding RNAs (ncRNAs) form a vast class of RNAs that do not code for protein. Examples of ncRNAs include transfer RNA (tRNA), ribosomal RNA (rRNA), small nuclear RNA (snRNA), small nucleolar RNA (snoRNA), microRNA (miRNA), PIWIinteracting RNA (piRNA), endogenous small interfering RNA (siRNA), circular RNA (circRNA), long non-coding RNA (lncRNA), protein functional effector small ncRNA (pfeRNA), and other ncRNAs whose functions remain unknown [21,22]. They can control the expression of genes at the chromosomal, transcriptional, post-transcriptional, and translational levels and play a role in the entire developmental process.…”

Section: Introductionmentioning

confidence: 99%

Non-Coding RNAs Potentially Involved in Pyrethroid Resistance of Anopheles funestus Population in Western Kenya

Debrah,

Zhong,

Machani

et al. 2024

Preprint

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Backgrounds The resurgence of Anopheles funestus, a dominant vector of human malaria in western Kenya was partly attributed to insecticide resistance. However, evidence on the molecular basis of pyrethroid resistance in western Kenya is limited. Noncoding RNAs (ncRNAs) form a vast class of RNAs that do not code for proteins and are ubiquitous in the insect genome. Here, we demonstrated that multiple ncRNAs could play a potential role in An. funestusresistance to pyrethroid in western Kenya. Materials and Methods Anopheles funestus mosquitoes were sampled by aspiration methods in Bungoma, Teso, Siaya, Port Victoria and Kombewa in western Kenya. The F1 progenies were exposed to deltamethrin (0.05%), permethrin (0.75%), DDT (4%) and pirimiphos-methyl (0.25%) following WHO test guidelines. A synergist assay using piperonyl butoxide (PBO) (4%) was conducted to determine cytochrome P450s' role in pyrethroid resistance. RNA-seq was conducted on a combined pool of specimens that were resistant and unexposed, and the results were compared with those of the FANG susceptible strain. This approach aimed to uncover the molecular mechanisms underlying pyrethroid resistance. Results Pyrethroid resistance was observed in all the sites with an average mortality rate of 57.6%. Port Victoria had the highest level of resistance to permethrin (MR=53%) and deltamethrin (MR=11%) pyrethroids. Teso had the lowest level of resistance to permethrin (MR=70%) and deltamethrin (MR=87%). Resistance to DDT was observed only in Kombewa (MR=89%) and Port Victoria (MR=85%). A full susceptibility to P-methyl (0.25%) was observed in all the sites. PBO synergist assay revealed high susceptibility (>98%) to the pyrethroids in all the sites except for Port Victoria (MR=96%, n=100). Whole transcriptomic analysis showed that most of the gene families associated with pyrethroid resistance comprised non-coding RNAs (67%), followed by imipenemase (10%),cytochrome P450s (6%), cuticular proteins (5%), olfactory proteins (4%), glutathione S-transferases (3%), UDP-glycosyltransferases (2%), ATP-binding cassettes (2%) and carboxylesterases(1%). Conclusions This study unveils the molecular basis of insecticide resistance in An. funestus in western Kenya, highlighting for the first time the potential role of non-coding RNAs in pyrethroid resistance. Targeting non-coding RNAs for intervention development could help in insecticide resistance management.

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Section: Introductionmentioning

confidence: 99%

Non-Coding RNAs Potentially Involved in Pyrethroid Resistance of Anopheles funestus Population in Western Kenya

Debrah,

Zhong,

Machani

et al. 2024

Preprint

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“…NcRNA is an umbrella term under which fall several subtypes that play significant roles in cellular functions. NcRNAs are typically divided by their length in long non-coding RNAs (lncRNAs), which represent transcripts more than 200 bp long, and small ncRNAs [such as microRNAs (miRNAs, miRs), small nuclear RNAs (snRNAs), small inhibitory RNAs (siRNAs) and Piwi-interacting RNAs (piRNAs], which are shorter than 200 bp [ 11 ]. A plethora of studies have supported the biological relevance of lncRNAs and underlined their central functions in both physiological and pathological conditions, such as cell differentiation and proliferation, immune response, tumor genesis, and tumor invasion and migration [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of EMT-Related lncRNAs in Ovarian Cancer

Lampropoulou¹,

Papadimitriou

et al. 2023

IJMS

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Ovarian cancer (OC) is one of the deadliest cancers worldwide; late diagnosis and drug resistance are two major factors often responsible for high morbidity and treatment failure. Epithelial-to-mesenchymal transition (EMT) is a dynamic process that has been closely linked with cancer. Long non-coding RNAs (lncRNAs) have been also associated with several cancer-related mechanisms, including EMT. We conducted a literature search in the PubMed database in order to sum up and discuss the role of lncRNAs in regulating OC-related EMT and their underlying mechanisms. Seventy (70) original research articles were identified, as of 23 April 2023. Our review concluded that the dysregulation of lncRNAs is highly associated with EMT-mediated OC progression. A comprehensive understanding of lncRNAs’ mechanisms in OC will help in identifying novel and sensitive biomarkers and therapeutic targets for this malignancy.

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“…Non-coding RNAs (ncRNAs) are a large class of RNAs with no protein products, including transfer RNA (tRNA), ribosomal RNA (rRNA), small nuclear RNA (snRNA), small nucleolar RNA (snoRNA), microRNA (miRNA), PIWI-interacting RNA (piRNA), endogenous small interfering RNA (siRNA), circular RNA (circRNA), long non-coding RNA (lncRNA), protein functional effector small ncRNA (pfeRNA) and other ncRNAs with unknown functions [1,2]. ncRNAs have been identified in bacteria and humans and are multi-faced.…”

Section: Introductionmentioning

confidence: 99%

Non-coding RNAs in Lepidoptera

Li,

Kan,

2023

Biodiversity and Ecology of Lepidoptera - Insights and Advances

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In the last few years, the amount of genomic sequence data has grown exponentially. A large number of non-coding RNAs (ncRNAs) have been identified from bacteria to humans. ncRNAs are various and multi-faced; they can regulate gene expression through chromosomal, transcriptional, post-transcriptional, and translational levels and then participate in the whole process of development in different organisms. ncRNAs have been identified in the 1980s in Lepidoptera; they can play roles in growth, metamorphosis, metabolism, sex determination, reproduction, and immune response of insects. Now, the use of ncRNAs in pest control of Lepidoptera is also in process. This chapter will review the recent advance of ncRNAs in Lepidoptera and prospect the future studies of ncRNAs in insects.

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pfeRNAs—A Novel Class of Small Non-coding RNAs With Real Translational Potential

Cited by 3 publications

References 38 publications

Non-Coding RNAs Potentially Involved in Pyrethroid Resistance of Anopheles funestus Population in Western Kenya

Non-Coding RNAs Potentially Involved in Pyrethroid Resistance of Anopheles funestus Population in Western Kenya

The Role of EMT-Related lncRNAs in Ovarian Cancer

Non-coding RNAs in Lepidoptera

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