PGMD/curcumin nanoparticles for the treatment of breast cancer

Abstract

The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was − 18.9 and − 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75–81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC50 of the nanoformulations was observed to be 40.2 and 33.6 μM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 μM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis.

Effects of Curcumin-Loaded Poly(Lactic-Co-Glycolic Acid) Nanoparticles in MDA-MB231 Human Breast Cancer Cells

“…On increasing the treatment duration, the rate of migration of MDA-MB231 cells was reduced greatly as compared with the controls (cells alone, PLGA-blank NPs and naked curcumin) as shown in Figure 5. Interestingly, from the images which were captured during the migration studies, it was observed that free curcumin did not contribute significantly towards affecting the migration of the cells (Figures 6 & 7) as stated by Kumari et al [18]. Free curcumin, instead, killed the cells rather than affecting the migration of cells.…”

Prodrug Polymeric Nanoconjugates Encapsulating Gold Nanoparticles for Enhanced X‐Ray Radiation Therapy in Breast Cancer

“…CUR has been shown to suppress cell growth in different cancer cells in the past. [ 32,33 ] However, compared to CUR, mPEG‐CUR and mPEG‐CUR@Au caused higher cytotoxicity. As demonstrated in Figure 3c, drug combinations with X‐ray irradiation eliminated more cancer cells than CUR, and mPEG‐CUR at each concentration.…”

Electrospun Coaxial Fibers to Optimize the Release of Poorly Water-Soluble Drug

“…The IR spectra of raw materials and drug-loaded fibers were obtained to explore the possible compatibility of components ( Figure 3 b). The characteristic peaks at 3510, 1602, 1506, and 1152 cm −1 of Cur demonstrate hydroxyl (-OH), carbonyl (-C=O), olefins (-C=C-), and ether (-C-O) groups, respectively [ 45 , 57 , 58 ]. The characteristic -C=O groups of PHBV and PVP were observed in 1720 and 1649 cm −1 , respectively [ 59 , 60 ].…”