pH‐Induced Symmetry Conversion of DNA Origami Lattices

Abstract

DNA nanotechnology has provided credible approaches for assembly of three-dimensional (3D) lattices with complex patterns. However, the symmetries are strictly dependent on their initial configurations and difficult to alter via non-thermal treatments. While switchable nucleic acid structures have been employed to construct deformable DNA motifs, it remains challenging to arrange them anisotropically in 3D lattices to trigger directed collective shape transition and dynamic symmetry conversion. In this work, we used octahedral DNA origami frames to synthesize four DNA origami lattices by placing the pH-reactive i-motif sequences in the desired dimensions. Thereinto, lattices with an anisotropic design can switch between simple cubic (SC) and simple tetragonal (ST) upon pH change. Small angle X-ray scattering (SAXS) results reveal the feasibility of obtaining 3D lattices with sensitive responses to external stimuli, expanding the way to obtain low-symmetry lattices.

Recycling Materials for Sustainable DNA Origami Manufacturing

“…While recycling of DON components for completely new structures has not previously been reported, several studies have developed designs where structure conformations can be adjusted through actuation mechanisms such as change of pH, temperature, or salt concentration. (26)(27)(28) However, these actuation and partial refolding strategies lead to only small changes or local adjustments to the conformation and do not allow complete reprogramming into a new structure with new properties. (26,(29)(30)(31) Several studies have investigated folding DONs with staple excesses below 10x.…”