pH-metric detection of alkaline phosphatase activity as a novel biosensing platform

Koncki, Robert; Rozum, Beata; Głąb, Stanisław

doi:10.1016/j.talanta.2005.07.002

Cited by 14 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sodium monofluorophosphate (MFP) has been used as substrate for pH‐metric detection of alkaline phosphatase activity [45], but its usage as nanozyme substrate to realize pH‐metric detection has not been reported. To validate the detection result, we first investigated how the concentration of CeO 2 NRs affects the pH value by incubating different concentrations of CeO 2 NRs with 5 mM MFP as well as CeO 2 NRs alone (Figure 3A), of which the latter condition poses negligible influence on pH (Figure 3A, red curve).…”

Section: Resultsmentioning

confidence: 99%

An Aptasensing Strategy Using the Phosphatase‐mimic Nanozyme and pH Meter as Signal Readout

Shang

Wang

et al. 2021

Electroanalysis

View full text Add to dashboard Cite

The demand for point‐of‐care testing (POCT) is growing dramatically, especially for district where health facilities are poorly staffed, poorly skilled and ill‐equipped. As a commercialized portable device, pH meters can be used for detection of various targets, relying on bioactive enzymes. The nanozymes, as the alternative of the natural enzymes, have rarely been used for pH‐metric POCT strategies. Herein, we developed an ultrasensitive pH‐metric sandwich‐type aptasensor based on the CeO2 nanorods (CeO2 NRs) as phosphatase‐mimic and sodium monofluorophosphate (MFP) as catalytic substrate. Under optimal conditions, such strategy yields a detection limit of 1.17 nM with eligible selectivity for detecting thrombin.

show abstract

Section: Resultsmentioning

confidence: 99%

An Aptasensing Strategy Using the Phosphatase‐mimic Nanozyme and pH Meter as Signal Readout

Shang

Wang

et al. 2021

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…Capillary electrophoresis (CE) is applicable in the analysis of a wide range of compounds including natural products and plant metabolites [24]. Several analytical methods were used to determinate enzymatic activities in different biological samples [25][26][27]. Nowadays, the use of capillary electrophoresis (CE) in enzyme assays has advantages over conventional assays in terms of time of analysis, and sample size required.…”

Section: Introductionmentioning

confidence: 99%

Determination of the β-Glucosidase Activity in Different Soils by Pre Capillary Enzyme Assay Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

et al. 2009

View full text Add to dashboard Cite

Enzyme activities can provide indication for quantitative changes in soil organic matter (SOM). It is known that the activities of most enzymes increase as native SOM content reflecting larger microbial communities and stabilization of enzymes on humic materials. Beta-glucosidase (beta-Glu) activities have been frequently used as indicators of changes in quantity and quality of SOM. In this study we propose a simple and very sensitive method, which has lower limit of detection compared with classic spectrophotometric method with the aim of determinate the beta-Glu activity in soil samples using Fluorescein mono-beta-D-glucopyranoside (FMGlc) as a substrate. The fluorescein released by the enzymatic reaction was quantified by capillary electrophoresis-laser induced fluorescence (CE-LIF) method. The background electrolyte (BGE) consisted in 40 mM phosphate buffer, pH 6. The LOD and LOQ for fluorescein were 1.3 10(-7) mg mL(-1) and 6.4 10(-6) mg mL(-1), respectively. This work deals with the minimization of the mixture for the enzymatic reaction and with the optimization conditions of CE separation. To the best of our knowledge, this is the first time that an enzymatic activity was detected in soil using CE-LIF system.

show abstract

“…Several analytical methods were used to determinate enzymatic activities in different biological samples [20][21][22]. Nowadays, the use of capillary electrophoresis (CE) in enzyme assays has advantages over conventional assays in terms of time of analysis, and sample size required [23].…”

mentioning

confidence: 99%

Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil

Stege

Messina

Sombra

et al. 2009

Biochemical Engineering Journal

View full text Add to dashboard Cite

show abstract

pH-metric detection of alkaline phosphatase activity as a novel biosensing platform

Cited by 14 publications

References 29 publications

An Aptasensing Strategy Using the Phosphatase‐mimic Nanozyme and pH Meter as Signal Readout

An Aptasensing Strategy Using the Phosphatase‐mimic Nanozyme and pH Meter as Signal Readout

Determination of the β-Glucosidase Activity in Different Soils by Pre Capillary Enzyme Assay Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil

Contact Info

Product

Resources

About