pH-responsive and porous vancomycin-loaded PLGA microspheres: evidence of controlled and sustained release for localized inflammation inhibition <i>in vitro</i>

Yu, Xiaoling; Pan, Qingqing; Zheng, Zongfu; Chen, Yongzhong; Chen, Yu-Yuan; Weng, Shaohuang; Huang, Liying

doi:10.1039/c8ra06659k

Cited by 19 publications

(11 citation statements)

References 46 publications

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“…Secondly, vancomycin is a hydrophilic antibiotic and is convenient to work with in aqueous media, along with being adsorbed onto HAp more effectively than its hydrophobic analogues while remaining loadable into PLGA. A number of studies have reported on the successful loading and delivery of vancomycin with the use of PLGA spheres [ 48 , 49 , 50 ]. Overall, the drug release results confirm that the addition of the HAp shell around the PLGA spheres does not impede the release from the polymer.…”

Section: Resultsmentioning

confidence: 99%

When Nothing Turns Itself Inside out and Becomes Something: Coating Poly(Lactic-Co-Glycolic Acid) Spheres with Hydroxyapatite Nanoparticles vs. the Other Way Around

Uskoković

Wu²

2022

JFB

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To stabilize drugs physisorbed on the surface of hydroxyapatite (HAp) nanoparticles and prevent burst release, these nanoparticles are commonly coated with polymers. Bioactive HAp, however, becomes shielded from the surface of such core/shell entities, which partially defeats the purpose of using it. The goal of this study was to assess the biological and pharmacokinetic effects of inverting this classical core/shell structure by coating poly(lactic-co-glycolic acid) (PLGA) spheres with HAp nanoparticles. The HAp shell did not hinder the release of vancomycin; rather, it increased the release rate to a minor degree, compared to that from undecorated PLGA spheres. The decoration of PLGA spheres with HAp induced lesser mineral deposition and lesser upregulation of osteogenic markers compared to those induced by the composite particles where HAp nanoparticles were embedded inside the PLGA spheres. This was explained by homeostatic mechanisms governing the cell metabolism, which ensure than the sensation of a product of this metabolism in the cell interior or exterior is met with the reduction in the metabolic activity. The antagonistic relationship between proliferation and bone production was demonstrated by the higher proliferation rate of cells challenged with HAp-coated PLGA spheres than of those treated with PLGA-coated HAp. It is concluded that the overwhelmingly positive response of tissues to HAp-coated biomaterials for bone replacement is unlikely to be due to the direct induction of new bone growth in osteoblasts adhering to the HAp coating. Rather, these positive effects are consequential to more elementary aspects of cell attachment, mechanotransduction, and growth at the site of contact between the HAp-coated material and the tissue.

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Section: Resultsmentioning

confidence: 99%

When Nothing Turns Itself Inside out and Becomes Something: Coating Poly(Lactic-Co-Glycolic Acid) Spheres with Hydroxyapatite Nanoparticles vs. the Other Way Around

Uskoković

Wu²

2022

JFB

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“…Protonation can induce conformational changes in peptides that destabilize lipids, promoting payload release, or facilitate insertion into biological lipid membranes, enhancing endosomal escape and payload release into the cytosol [ 138 , 474 ], Acid-induced degradation of inorganics such as calcium phosphate (Ca 3 (PO 4 ) 2 ) shells or zinc oxide (ZnO) gatekeepers allow the encapsulated payloads to be released at a pH below 5.0 [ 459 ]. The CO 2 pressure that results from the acid-induced degradation of NH 4 HCO 3 has been demonstrated to be sufficient to crack polymeric shells, enhancing payload release [ 457 ]. Recent advances in the application of these techniques to the various platforms discussed in Section 2 are reviewed below.…”

Section: Stimulimentioning

confidence: 99%

Nanoplatforms for Targeted Stimuli-Responsive Drug Delivery: A Review of Platform Materials and Stimuli-Responsive Release and Targeting Mechanisms

Sun

Davis

2021

Nanomaterials

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To achieve the promise of stimuli-responsive drug delivery systems for the treatment of cancer, they should (1) avoid premature clearance; (2) accumulate in tumors and undergo endocytosis by cancer cells; and (3) exhibit appropriate stimuli-responsive release of the payload. It is challenging to address all of these requirements simultaneously. However, the numerous proof-of-concept studies addressing one or more of these requirements reported every year have dramatically expanded the toolbox available for the design of drug delivery systems. This review highlights recent advances in the targeting and stimuli-responsiveness of drug delivery systems. It begins with a discussion of nanocarrier types and an overview of the factors influencing nanocarrier biodistribution. On-demand release strategies and their application to each type of nanocarrier are reviewed, including both endogenous and exogenous stimuli. Recent developments in stimuli-responsive targeting strategies are also discussed. The remaining challenges and prospective solutions in the field are discussed throughout the review, which is intended to assist researchers in overcoming interdisciplinary knowledge barriers and increase the speed of development. This review presents a nanocarrier-based drug delivery systems toolbox that enables the application of techniques across platforms and inspires researchers with interdisciplinary information to boost the development of multifunctional therapeutic nanoplatforms for cancer therapy.

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“…A recent study showed that pH value can regulate drug release rate, with a more acidic pH value releasing the microsphere-entrapped drugs more quickly. 24 In the current study, the drug release rate of MMC-loaded PPM at pH 6.5 was faster than that at pH 7.4, suggesting that MMC-loaded PPM had potential for tumor targeting. A549 cells were injected subcutaneously into C57BL/6 nude mice to detect the anti-tumor function of MMCloaded PPM on tumor growth in vivo.…”

mentioning

confidence: 46%

Polysaccharide-Based Lotus Seedpod Surface-Like Porous Microsphere as an Efficient Drug Carrier for Cancer Treatment

Zhang

et al. 2021

CMAR

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Background: This study aimed to evaluate the properties and functions of polysaccharidebased porous microsphere (PPM) for drug delivery, as well as its inhibitory effect on malignant tumors. Materials and Methods: PPM was prepared using the inverse emulsion polymerization method. FT-IR measurements were conducted to measure the wavenumber of PPM. Particle size distribution was tested with a particle analyzer, and surface morphologies of PPM were observed using a scanning electron microscope (SEM). Dialysis method, Cell Counting Kit-8 (CCK-8), and cell apoptosis analysis were adopted to evaluate the drug release, cytotoxicity and biocompatibility of mitomycin-C (MMC), respectively. Finally, an in vivo study was performed in C57BL/6 mice to confirm the function of MMC-loaded PPM on tumor growth. Results: FT-IR spectra proved the successful preparation of MMC-loaded PPM. PPM had an average size of 25.90 ± 0.34 μm and then increased to 30.10 ± 0.20 μm after drug loading. Under SEM, the surface morphology was lotus seedpod surface-like, with macropits on the surface and micropores in macropits. Compared with the free MMC group, MMC-loaded PPM exhibited a delayed drug release rate in a pH-dependent manner and higher cell viability. Flow cytometry results showed that the cell apoptosis in the PPM/MMC group was lower than that in the free MMC group. In vivo experiment revealed the inhibitory efficacy of MMC-loaded PPM on malignant tumors. Conclusion: In summary, MMC-loaded PPM exhibited favorable surface morphology, sustained drug release ability, nontoxicity and excellent biocompatibility, suggesting that PPM might be a potential drug carrier for tumor treatment.

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pH-responsive and porous vancomycin-loaded PLGA microspheres: evidence of controlled and sustained release for localized inflammation inhibition in vitro

Abstract: pH-responsive and porous vancomycin-loaded PLGA microspheres were developed for adequate delivery of antibiotics to infected sites.

Cited by 19 publications

References 46 publications

When Nothing Turns Itself Inside out and Becomes Something: Coating Poly(Lactic-Co-Glycolic Acid) Spheres with Hydroxyapatite Nanoparticles vs. the Other Way Around

When Nothing Turns Itself Inside out and Becomes Something: Coating Poly(Lactic-Co-Glycolic Acid) Spheres with Hydroxyapatite Nanoparticles vs. the Other Way Around

Nanoplatforms for Targeted Stimuli-Responsive Drug Delivery: A Review of Platform Materials and Stimuli-Responsive Release and Targeting Mechanisms

Polysaccharide-Based Lotus Seedpod Surface-Like Porous Microsphere as an Efficient Drug Carrier for Cancer Treatment

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