pH-Responsive Graphene Oxide–DNA Nanosystem for Live Cell Imaging and Detection

Chuang, Shao Yuan; Liang, Jia; He, Sihui; Luan, Tianqi; Yu, Jiantao; Zhao, Haoran; Xu, Jingyuan; Tian, Leilei

doi:10.1021/acs.analchem.7b00369

Cited by 35 publications

(32 citation statements)

References 47 publications

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“…In this work, we aimed to determine the effects of mono-GO and multi-GO on DCs using in vitro cell model. According to bioapplication and various toxicity studies of GO (Shao et al, 2017;Hoyle et al, 2018;Pelin et al, 2018;Gurunathan et al, 2019a), we finally selected concentrations of 0.01 mg/ml, 0.1 mg/ml, 1 mg/ml, 10 mg/ml, and 100 mg/ml for in vitro toxicity tests. Here, our results showed mono-GO is less toxic to DCs than multi-GO.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Yang

Pan²,

Chen

et al. 2020

Front. Pharmacol.

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Graphene, known as "black gold", has important applications in various fields. In previous studies, it has been proved that graphene oxide (GO) which is a derivative of graphene has low toxicity. However, the immunotoxicity of GO has not been fully elucidated. In this work, we used DC2.4 cell line to investigate the in vitro immunotoxicity of two types of GO, mono-layer GO (mono-GO) and multi-layer GO (multi-GO). We found that mono-GO had less effect on cell viability than multi-GO, but both mono-GO and multi-GO significantly induced the generation of ROS in DC2.4 cells. Interestingly, mono-GO caused DC2.4 cells to aggregate, thus changed the cell morphology significantly. However, no similar influence occurred for multi-GO. In addition, the results showed that these two GOs obviously enhance the release of TNF-a by DC2.4 cells with and without LPS stimulation. GO did not affect the level of IL-6 released from DC2.4 cells, but multi-GO promoted the release of IL-6 while mono-GO inhibited the production of IL-6 when cells were in response to LPS stimulation. Whole-transcriptome sequencing analysis found some immune-related differentially expressed genes including H2-DMb1, Ncbp3, Oas2, Men1, Fas, Cd320, Cd244, and Tinagl1 which are engaged in the immune system process. These results suggested that both mono-GO and multi-GO are immunotoxic to DC2.4 cells, which provides important basis for subsequent biological and clinical medical applications.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Yang

Pan²,

Chen

et al. 2020

Front. Pharmacol.

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“…First, as the interaction between GO and DNA is sensitive to pH, it is hard to develop pH sensors based on this method. [167] Second, there will be a severe specificity problem when the physisorbed sensor is applied to intracellular detection. As the intracellular environment contains a high concentration of nucleic acids and proteins, the physically adsorbed DNA might be displaced by the nontarget molecules.…”

Section: Go-dna Complexmentioning

confidence: 99%

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

et al. 2020

Self Cite

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The comprehensive understanding and proper use of supramolecular interactions have become critical for the development of functional materials, and so is the biomedical application of nucleic acids (NAs). Relatively rare attention has been paid to hydrophobic interaction compared with hydrogen bonding and electrostatic interaction of NAs. However, hydrophobic interaction shows some unique properties, such as high tunability for application interest, minimal effect on NA functionality, and sensitivity to external stimuli. Therefore, the widespread use of hydrophobic interaction has promoted the evolution of NA-based biomaterials in higher-order self-assembly, drug/gene-delivery systems, and stimuli-responsive systems. Herein, the recent progress of NA-based biomaterials whose fabrications or properties are highly determined by hydrophobic interactions is summarized. 1) The hydrophobic interaction of NA itself comes from the accumulation of base-stacking forces, by which the NAs with certain base compositions and chain lengths show properties similar to thermal-responsive polymers. 2) In conjugation with hydrophobic molecules, NA amphiphiles show interesting self-assembly structures with unique properties in many new biosensing and therapeutic strategies. 3) The working-mechanisms of some NA-based complex materials are also dependent on hydrophobic interactions. Moreover, in recent attempts, NA amphiphiles have been applied in organizing macroscopic self-assembly of DNA origami and controlling the cell-cell interactions.

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“…Interfacing C‐rich DNA on GO with pH can also be used to make pH‐dependent switches (Figure 6B), exploiting the difference in binding affinity due to i‐motif formation. [ 69,70 ] Random/sensing DNA strands simply adsorbed on GO are most effective at neutral pH (Figure 6C), [ 57 ] since the interaction at low pH is too strong for probe to desorb. Similar designs are also applicable to other nanomaterials interacting with the DNA phosphate backbone.…”

Section: Discussionmentioning

confidence: 99%

Poly‐Cytosine Deoxyribonucleic Acid Strongly Anchoring on Graphene Oxide Due to Flexible Backbone Phosphate Interactions

Lopez

Zhao

Huang

et al. 2020

Adv Materials Inter

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Finding DNA sequences that can strongly adsorb on various nanomaterials is critically important for preparing bioconjugates, biosensors, and drug delivery. Poly‐cytosine (poly‐C) DNA is found to have stronger affinity compared to other DNA sequences of the same length on various nanomaterials ranging from graphene oxide (GO), MoS2, to many metal oxides and phosphates. In this work, the authors aim to understand the reason for such high affinity by varying pH and DNA sequence along with conducting molecular dynamics (MD) simulations using GO as a model surface. Poly‐C DNA adsorbs stronger only at neutral or basic pH, while its adsorption at acidic pH is weaker than other DNA homopolymers. The DNA sequence is further varied by inserting thymine into poly‐C DNA and by varying thymine/cytosine ratios, all confirming that a folded i‐motif structure is detrimental for adsorption. Using MD simulations, the authors reveal that the stronger adsorption of poly‐C DNA at neutral pH is due to more contributions from the phosphate backbone hydrogen bonding with GO surface relating to the flexibility of the DNA. Poly‐C DNA also uses its phosphate backbone to interact with metal oxide and phosphate nanoparticles, and this phosphate backbone interaction can unify all these observations.

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pH-Responsive Graphene Oxide–DNA Nanosystem for Live Cell Imaging and Detection

Cited by 35 publications

References 47 publications

Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

Poly‐Cytosine Deoxyribonucleic Acid Strongly Anchoring on Graphene Oxide Due to Flexible Backbone Phosphate Interactions

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