Phage Application for the Protection from Acute Hepatopancreatic Necrosis Disease (AHPND) in Penaeus vannamei

Jun, Jin Woo; Han, Jee Eun; Giri, Sib Sankar; Tang, Kathy F.J.; Zhou, Xiaohui; Aranguren, Luis Fernando; Kim, Hyoun Joong; Yun, Sukyoung; Chi, Cheng; Kim, Sang Guen

doi:10.1007/s12088-017-0694-9

Cited by 59 publications

(29 citation statements)

References 10 publications

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“…Culot, et al Aquaculture 513 (2019) 734423 In most of published data, timing has proved to be a very important factor regarding phage therapy. Mortality typically increases heavily as treatment time reaches a few hours post infection (Jun et al, 2018;Lomelí-Ortega and Martínez-Díaz, 2014;Martínez-Díaz and Hipólito-Morales, 2013).The best choice seems to administer phages before the infection starts and continue thereafter, as a prophylactic and therapeutic treatment. This need for an early administration might be linked to the time necessary for the phages to reproduce and become sufficiently concentrated in order to make their host's population collapse before the bacterial infection reach an irreversible situation.…”

Section: How To Ideally Use Phages?mentioning

confidence: 99%

Overcoming the challenges of phage therapy for industrial aquaculture: A review

2019

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Aquaculture is the fastest-growing sector in food industry. Its development is powered by the intensification of the production which increased bacterial disease occurrence and spreading. As aquaculture deeply relies on a massive prophylactic and therapeutic use of antibiotics, it is threatened by the emergence of multi drug resistant bacteria. The stalled development of new antibiotics makes finding new therapeutic solutions a burning issue. Thanks to their specific host range, their ability to treat both the farmed species and the environment, their limited ecological impact and their abundance in the environment, bacteriophages represent a promising sustainable solution to control pathogenic aquaculture bacteria. In this review we discuss the interest of phage biocontrol for aquaculture and how can bacterial resistance, ecological, pharmacological and production related issues be solved.

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Section: How To Ideally Use Phages?mentioning

confidence: 99%

Overcoming the challenges of phage therapy for industrial aquaculture: A review

2019

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“…For pH stability, 100 μl of phage lysate was mixed with 900 μl of SM buffer in a pH range 2 to 10 and incubated at 30 °C for 1 hour. For temperature stability, 50 μl of phage lysate was incubated for 1 hour at different temperatures; 4,20,25,30,37,40,50,60, and 70 °C. For both tests, phage infectivity was determined by performing a spot test.…”

Section: Methodsmentioning

confidence: 99%

A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery

Thammatinna

Egan

Htoo

et al. 2020

Sci Rep

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Since the emergence of deadly pathogens and multidrug-resistant bacteria at an alarmingly increased rate, bacteriophages have been developed as a controlling bioagent to prevent the spread of pathogenic bacteria. One of these pathogens, disease-causing Vibrio parahaemolyticus (Vp AHPND ) which induces acute hepatopancreatic necrosis, is considered one of the deadliest shrimp pathogens, and has recently become resistant to various classes of antibiotics. Here, we discovered a novel vibriophage that specifically targets the vibrio host, VP AHPND . The vibriophage, designated Seahorse, was classified in the family Siphoviridae because of its icosahedral capsid surrounded by head fibers and a non-contractile long tail. Phage Seahorse was able to infect the host in a broad range of pH and temperatures, and it had a relatively short latent period (nearly 30 minutes) in which it produced progeny at 72 particles per cell at the end of its lytic cycle. Upon phage infection, the host nucleoid condensed and became toroidal, similar to the bacterial DNA morphology seen during tetracycline treatment, suggesting that phage Seahorse hijacked host biosynthesis pathways through protein translation. As phage Seahorse genome encodes 48 open reading frames with many hypothetical proteins, this genome could be a potential untapped resource for the discovery of phage-derived therapeutic proteins.Vibrio is a genus of motile Gram-negative bacteria that possesses a curved-rod cell shape with a single flagellum. Vibrios are abundant and diverse bacteria that are typically found in marine habitats. The genus Vibrio consists of 14 recognized clades and at least 86 different species 1 . While some of them are not pathogenic, many can cause serious health effects in both human and aquatic life. Due to the continuously rising ocean temperature, the composition of vibrio in the ocean microbiome has been reported to be higher than usual 2-4 . This vibrio-rich environment might increase the incident of a vibrio outbreak in the near future posing risks to global health 2 .Vibrio parahaemolyticus, which is one of the disease-causing Vibrio species, is pathogenic to both humans and marine animals 5 . Consumption of raw seafoods contaminated with the bacteria can cause acute gastroenteritis 5,6 . This opportunistic bacterium is also able to infect through an open wound which can lead to sepsis and, in rare cases, subsequent death in immunocompromised patients 7,8 . Moreover, V. parahaemolyticus that has acquired a plasmid encoding the deadly binary toxins PirA vp /PirB vp is even more virulent 9 . The V. parahaemolyticus strain harboring the plasmid has been found to cause a newly emerging disease in shrimp, known as acute hepatopancreatic necrosis disease (AHPND) 9 . Moreover, the AHPND-causing plasmid is also found to be transferable among other vibrios, increasing the chance of the disease spreading regionally and globally 10 . Unsurprisingly, the spread of AHPND has been reported in many countries, including China, Vietnam, Malaysia, Thailand, Me...

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“…In a follow‐up study, Jun et al . () demonstrated that prophylactic and prophylactic/therapeutic treatments (oral or immersion) with pVp‐1 phage protect shrimp (50%–100% survival) against V. parahaemolyticus ‐AHPND lethal challenge.…”

Section: Phages Against Bacteria Causing Ahpndmentioning

confidence: 99%

Control of AHPND by phages: a promising biotechnological approach

Angulo

Loera‐Muro

Trujillo

et al. 2018

Reviews in Aquaculture

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Vibrio parahaemolyticus, V. campbellii, V. owensii and V. harveyi are causative agents of Acute Hepatopancreatic Necrosis Disease (AHPND) in shrimp. AHPND is recently one of the most important diseases worldwide because of the high mortality and huge economic losses in shrimp farms. Antibiotic resistance has caused concern into finding other options to fight against pathogenic bacterial diseases. Currently, the use of lytic phages has reemerged as a promising strategy to combat bacterial pathogens. Likewise, modern molecular tools have been applied to modify phages by genetic engineering to express specific antigens and antibodies. This study reviewed recent research reports in the development of phages to control Vibrio spp. infections in shrimp and discusses different specific strategies, placing them in perspective to combat AHPND.

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Phage Application for the Protection from Acute Hepatopancreatic Necrosis Disease (AHPND) in Penaeus vannamei

Cited by 59 publications

References 10 publications

Overcoming the challenges of phage therapy for industrial aquaculture: A review

Overcoming the challenges of phage therapy for industrial aquaculture: A review

A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery

Control of AHPND by phages: a promising biotechnological approach

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