Phage applications for improving food safety and infection control in Egypt

El‐Shibiny, Ayman; El-Sahhar, Salma; Kasem, A.

doi:10.1111/jam.13500

Cited by 43 publications

(28 citation statements)

References 50 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results suggested that phage cocktail is a suitable candidate for biological control of Salmonella in foods. Other researchers also showed that the phage cocktail could control pathogenic bacteria in foods, biofilms and food safety quality [88,89].…”

Section: Discussionmentioning

confidence: 99%

“…Because the high stability of phage cocktail could reduce the growth rate of bacteria at that temperature [90,91]. It has been demonstrated that, using high concentrations of phages generally achieved high reduction rates of pathogens [74,88,92]. When high titers are applied, phages are capable to absorb to the bacterial cells causing lysis to the cytoplasmic membrane without replication [10,93], reported as a process known as "lysis from without" [94].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms

Islam

Zhou

Liang

et al. 2019

Viruses

117

112

View full text Add to dashboard Cite

Salmonella contamination in foods and their formation of biofilms in food processing facility are the primary bacterial cause of a significant number of foodborne outbreaks and infections. Broad lytic phages are promising alternatives to conventional technologies for pathogen biocontrol in food matrices and reducing biofilms. In this study, 42 Salmonella phages were isolated from environmentally-sourced water samples. We characterized the host range and lytic capacity of phages LPSTLL, LPST94 and LPST153 against Salmonella spp., and all showed a wide host range and broad lytic activity. Electron microscopy analysis indicated that LPSTLL, LPST94, and LPST153 belonged to the family of Siphoviridae, Ackermannviridae and Podoviridae, respectively. We established a phage cocktail containing three phages (LPSTLL, LPST94 and LPST153) that had broad spectrum to lyse diverse Salmonella serovars. A significant decrease was observed in Salmonella with a viable count of 3 log10 CFU in milk and chicken breast at either 25 °C or 4 °C. It was found that treatment with phage cocktail was able to significantly reduced biofilm on a 96-well microplate (44–63%) and on a stainless steel surface (5.23 to 6.42 log10). These findings demonstrated that the phage cocktail described in this study can be potentially used as a biological control agent against Salmonella in food products and also has the effect to reduce Salmonella formed biofilms.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms

Islam

Zhou

Liang

et al. 2019

Viruses

117

112

View full text Add to dashboard Cite

show abstract

“…Bacteriophages represent an alternative treatment for the control of bacterial contamination in foods as well as the control of bacterial infections in man and animals due to their abilities to specifically target bacterial host cells and self-replicating nature (Jassim and Limoges 2014; Summers 2001; Taha et al 2018). Research has demonstrated the use of bacteriophages to reduce E. coli O157:H7 in the gastrointestinal tracts of mice (Tanji et al 2005) and sheep (Bach et al 2003; Raya et al 2011), and on the surface of the meat (El-Shibiny et al 2017; O’Flynn et al 2004). Studies also suggest phage application could decrease the mortality rate of poultry on infected farms (Xie et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy

et al. 2019

View full text Add to dashboard Cite

Bacteriophages can be used successfully to treat pathogenic bacteria in the food chain including zoonotic pathogens that colonize the intestines of farm animals. However, harsh gastric conditions of low pH and digestive enzyme activities affect phage viability, and accordingly reduce their effectiveness. We report the development of a natural protective barrier suitable for oral administration to farm animals that confers acid stability before functional release of bead-encapsulated phages. Escherichia coli bacteriophage ZSEC5 is rendered inactive at pH 2.0 but encapsulation in chitosan–alginate bead with a honey and gelatin matrix limited titer reductions to 1 log 10 PFU mL −1 . The encapsulated phage titers were stable upon storage in water but achieved near complete release over 4–5 h in a simulated intestinal solution (0.1% bile salt, 0.4% pancreatin, 50 mM KH 2 PO 4 pH 7.5) at 37 °C. Exposure of E. coli O157:H7 to the bead-encapsulated phage preparations produced a delayed response, reaching a maximal reductions of 4.2 to 4.8 log 10 CFU mL −1 after 10 h at 37 °C under simulated intestinal conditions compared to a maximal reduction of 5.1 log 10 CFU mL −1 at 3 h for free phage applied at MOI = 1. Bead-encapsulation is a promising reliable and cost-effective method for the functional delivery of bacteriophage targeting intestinal bacteria of farm animals.

show abstract

“…Other studies have characterized a number of diverse lytic bacteriophages to K. pneumoniae belonging to different families and demonstrated their potential in vitro (Bogovazova et al, 1991 ; Kesik-Szeloch et al, 2013 ; Hoyles et al, 2015 ). Bacteriophage therapy is regarded as a simple, safe and highly effective alternative to counter the rising problems associated with multidrug resistant bacteria (Qadir, 2015 ; El-Shibiny et al, 2017 ). Here we evaluate the lytic activity of bacteriophage ZCKP1 isolated from an environmental freshwater source in Egypt against a MDR K. pneumoniae KP/01 isolated from the foot of a diabetic patient.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage ZCKP1: A Potential Treatment for Klebsiella pneumoniae Isolated From Diabetic Foot Patients

Taha

Connerton

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

The recorded growth in infection by multidrug resistant bacteria necessitates prompt efforts toward developing alternatives to antibiotics, such as bacteriophage therapy. Immuno-compromised patients with diabetes mellitus are particularly prone to foot infections by multidrug resistant Klebsiella pneumoniae, which may be compounded by chronic osteomyelitis. Bacteriophage ZCKP1, isolated from freshwater in Giza, Egypt, was tested in vitro to evaluate its lytic activity against a multidrug resistant K. pneumoniae KP/01, isolated from foot wound of a diabetic patient in Egypt. Characterization of ZCKP1 phage indicated that it belonged to the Myoviridae family of bacteriophages with a ds-DNA genome size of 150.9 kb. Bacteriophage ZCKP1 lysed a range of osteomyelitis pathogenic agents including Klebsiella spp., Proteus spp. and E. coli isolates. The bacteriophage reduced the bacterial counts of host bacteria by ≥2 log10 CFU/ml at 25°C, and demonstrated the ability to reduce bacterial counts and biofilm biomass (>50%) when applied at high multiplicity of infection (50 PFU/CFU). These characteristics make ZCKP1 phage of potential therapeutic value to treat K. pneumoniae and associated bacteria present in diabetic foot patients.

show abstract

Phage applications for improving food safety and infection control in Egypt

Abstract: The results suggest that phages could be effective treatments for pathogenic bacteria in food relevant contexts in Egypt.

Cited by 43 publications

References 50 publications

Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms

Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms

Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy

Bacteriophage ZCKP1: A Potential Treatment for Klebsiella pneumoniae Isolated From Diabetic Foot Patients

Contact Info

Product

Resources

About